Interaction of human immunoglobulin G with l-histidine immobilized onto poly(ethylene vinyl alcohol) hollow-fiber membranes

Haupt, Karsten; Bueno, Sônia Maria Alves; Vijayalakshmi, M. A.

doi:10.1016/0378-4347(95)00282-9

Cited by 58 publications

(32 citation statements)

References 8 publications

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“…[19] To clarify this point adsorption studies were carried out using MES and phosphate buffers within their respective buffering ranges. Figure 5 shows the IgG adsorption-capacity of P(HEMA-co-MAH) beads in these buffer systems at different pH's.…”

Section: Effect Of Buffer Typementioning

confidence: 99%

A Novel Affinity Support Material for the Separation of Immunoglobulin G from Human Plasma

Gari̇pcan

Deni̇zli̇²

2002

Macromol. Biosci.

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2‐Methacrylamidohistidine (MAH) as a pseudospecific ligand was synthesized from methacryl chloride and histidine. Spherical beads with an average size of 50–63 μm were obtained by the radical suspension polymerization of MAH and 2‐hydroxyethyl methacrylate (HEMA) conducted in an aqueous dispersion medium. Owing to the reasonably rough character of the bead surface, P(HEMA‐co‐MAH) beads had a specific surface area of 17.6 m2·g–1. Synthesized MAH was characterized by NMR. P(HEMA‐co‐MAH) beads were characterized by swelling studies, FT‐IR spectroscopy, scanning electron microscopy (SEM) and elemental analysis. P(HEMA‐co‐MAH) affinity beads with a swelling ratio of 65% were used in the separation of human immunoglobulin G (HIgG) from aqueous solutions and human plasma. The maximum HIgG adsorption on the P(HEMA‐co‐MAH) adsorbents was observed at pH 7.4 for phosphate and at pH 6.0 for morpholinoethanesulfonic acid buffers. The HIgG adsorption onto the PHEMA adsorbents was negligible. Higher adsorption values (up to 46.5 mg·g–1) were obtained when the P(HEMA‐co‐MAH) adsorbents were used in aqueous solutions. Much higher amounts of HIgG were adsorbed from human plasma (up to 73.8 mg·g–1). Adsorption capacities of other blood proteins were obtained as 3.2 mg·g–1 for fibrinogen and 4.6 mg·g–1 for albumin. The total protein adsorption was determined to be 82.2 mg·g–1. The pseudospecific affinity beads allowed one‐step separation of HIgG from human plasma. HIgG molecules could be repeatedly adsorbed and desorbed with these adsorbents without noticeable loss in their HIgG adsorption capacity.

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Section: Effect Of Buffer Typementioning

confidence: 99%

A Novel Affinity Support Material for the Separation of Immunoglobulin G from Human Plasma

Gari̇pcan

Deni̇zli̇²

2002

Macromol. Biosci.

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“…Generally, the pseudobiospecific ligands are low cost and have high stability, capacity, simplicity, and selectivity [17]. Among these ligands, the immobilized amino acid histidine is an interesting alternative for human IgG purification [18,19]. As an example, histidine grafted aminohexyl-Sepharose 4B was used as a negative affinity adsorbent for purification of IgG from human plasma diluted 20 times in Mops buffer at pH 7.2 [20].…”

Section: Introductionmentioning

confidence: 99%

Adsorption of human serum proteins onto TREN-agarose: Purification of human IgG by negative chromatography

Bresolin

Borsoi-Ribeiro

Rodrigues

et al. 2009

Journal of Chromatography B

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“…Here, the thiophilic mercaptoheterocyclic ligands were greatly influenced by buffer systems when the IgG adsorption was achieved in a salt independent chromatography. This influence was already shown for l-histidine ligand which had a maximal IgG adsorption in MOPS buffer [19,20]. It was shown that pseudo-biospecific affinity chromatography with l-histidine-PEVA membranes under zwitterionic buffers systems, like HEPES and MOPS, yielded much higher adsorption capacities in comparison with other buffers like Tris-HCl and PBS.…”

Section: Resultsmentioning

confidence: 58%

Mercaptoheterocyclic ligands grafted on a poly(ethylene vinyl alcohol) membrane for the purification of immunoglobulin G in a salt independent thiophilic chromatography

Coffinier

Vijayalakshmi

2004

Journal of Chromatography B

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Interaction of human immunoglobulin G with l-histidine immobilized onto poly(ethylene vinyl alcohol) hollow-fiber membranes

Cited by 58 publications

References 8 publications

A Novel Affinity Support Material for the Separation of Immunoglobulin G from Human Plasma

A Novel Affinity Support Material for the Separation of Immunoglobulin G from Human Plasma

Adsorption of human serum proteins onto TREN-agarose: Purification of human IgG by negative chromatography

Mercaptoheterocyclic ligands grafted on a poly(ethylene vinyl alcohol) membrane for the purification of immunoglobulin G in a salt independent thiophilic chromatography

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