Interaction of Hydrocortisone with Model Membranes Containing Phospholipid and Cholesterol

Cleary, Gary W.; Zatz, Joel L.

doi:10.1002/jps.2600660718

Cited by 21 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Early experimental data seemed to suggest that steroids do not incorporate within the membrane, but rather remain in the water phase outside the bilayer. [50][51][52] However, more recent measurements 22,53 clearly indicate that steroid hormones preferentially partition inside the bilayer; this is now the accepted view, 21 and is consistent with our simulation findings. Moreover, recent AL simulations of cortisone, another steroid hormone, also show a preferential partitioning inside the membrane, particularly corresponding to the headgroup-tail interface; 42 this is in agreement with our results.…”

Section: Transfer Free Energiessupporting

confidence: 91%

Permeability of drugs and hormones through a lipid bilayer: insights from dual-resolution molecular dynamics

2010

View full text Add to dashboard Cite

Section: Transfer Free Energiessupporting

confidence: 91%

Permeability of drugs and hormones through a lipid bilayer: insights from dual-resolution molecular dynamics

2010

View full text Add to dashboard Cite

“…perturber. In particular, this conclusion, which agrees with the structural analysis of Willmer ( 1961) and with the results of Tráuble & Sackmann (1972a,b) on spin-labeled steroids, contradicts an earlier conclusion drawn from film balance studies that steroids are not incorporated within the membrane (Gershfeld & Heftmann, 1963;Munck, 1957;Cleary & Zatz, 1977;Khaiat et al, 1975). The latter studies do not provide direct information on steroid localization.…”

Section: Resultsmentioning

confidence: 44%

Effects of anesthetic and nonanesthetic steroids on dipalmitoylphosphatidylcholine liposomes: a calorimetric and Raman spectroscopic investigation

O'Leary¹,

Ross²,

Levin³

1984

Biochemistry

View full text Add to dashboard Cite

The effects of anesthetic and nonanesthetic steroids on dipalmitoylphosphatidylcholine liposomes were studied by use of high sensitivity scanning calorimetry and Raman spectroscopy. Calorimetric measurements indicated that both anesthetic and nonanesthetic steroids depressed and broadened the gel to liquid-crystalline phase transition. There was no correlation between the perturbations by the steroids on the primary gel to liquid-crystalline phase transition temperature and anesthetic potency. The magnitudes of the steroid-induced transition broadening and lowering of the pretransition temperature, however, correlated well with anesthetic potency. This effect appeared to arise from the projection from the plane of the D ring of substituents at the C(17) position of the steroid nucleus. Raman spectroscopic measurements demonstrated that the steroid molecule is localized within the acyl region of the bilayer and that effects of the steroid do not extend to either the head-group or interface regions of the lamellae. The data are consistent with unitary hypotheses relating general anesthesia to lipid perturbations. For model systems, perturbations to the subtle structural and dynamical properties of the bilayer pretransition may provide a more sensitive marker than the main phase transition in assessing the significance of lipid mediation in inducing anesthetic action.

show abstract

“…Increase in surface pressure was dependent upon steroid concentration, and penetration of hydrocortisone was decreased by the addition of cholesterol to the monolayer system. It was concluded that cortisol interacts with the hydrated polar group of the phospholipid and not the hydrocarbon tail (169).…”

Section: Direct Effects Of Corticosteroids Upon Membranesmentioning

confidence: 99%

Corticosteroid-Induced Changes in Phospholipid Membranes as Mediators of Their Action*

Nelson

1980

Endocrine Reviews

View full text Add to dashboard Cite

T HIS SHORT review will describe mechanisms by which corticosteroid modification of cellular phospholipids and particularly the phospholipid-structure of a variety of membranes can produce some of the effects of the corticosteroids. In addition to these effects, many studies have demonstrated effects of other hormones to alter specific phospholipids, phospholipases, or for phospholipase action on cells to mimic hormone action. All actions of the corticosteroids are not thought to be produced by these lipid alterations, but such changes are capable of producing widespread effects on receptor activity, RNA and protein synthesis, enzymatic activity, and transport through membranes. The availability of new data indicating that phospholipids are altered considerably by corticosteroids in a number of tissues makes a review of this subject of interest at this time.Corticosteroids are secreted in response to a variety of "stresses" that represent alterations in the cellular environment that might result in death or injury to the exposed cells if proper adaptation did not occur. Thus, these hormones are increased in response to anoxia, very low or very high temperature, or hypoglycemia. Through mechanisms that have not been clearly understood the cell is protected by the increased hormones against potentially harmful alterations in its environment. Unicellular organisms, which lack a sophisticated endocrine system for response to stresses, undergo modifications in cellular membranes in response to stress. A decrease in the temperature at which the organism is grown would produce a decrease in the fluidity of the membrane with resulting marked effects upon membrane-bound enzymatic activities. Similarly, increased temperature would result in increased fluidity of the lipid membrane and similar alterations in its function. In order

show abstract

Interaction of Hydrocortisone with Model Membranes Containing Phospholipid and Cholesterol

Cited by 21 publications

References 20 publications

Permeability of drugs and hormones through a lipid bilayer: insights from dual-resolution molecular dynamics

Permeability of drugs and hormones through a lipid bilayer: insights from dual-resolution molecular dynamics

Effects of anesthetic and nonanesthetic steroids on dipalmitoylphosphatidylcholine liposomes: a calorimetric and Raman spectroscopic investigation

Corticosteroid-Induced Changes in Phospholipid Membranes as Mediators of Their Action*

Contact Info

Product

Resources

About