Interaction of insulin with colloidal ZnS quantum dots functionalized by various surface capping agents

Hosseinzadeh, Ghader; Farniya, Seyed Morteza Famil; Keihan, Amir Homayoun; Moosavi-Movahedi, Ali Akbar

doi:10.1016/j.msec.2017.04.018

Cited by 14 publications

(8 citation statements)

References 61 publications

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“…The values of Δ H ° and Δ S ° were calculated using the van’t Hoff equation where R and T are the gas constant and the absolute temperature, respectively. The values of Δ H ° and Δ S ° were obtained from the slope and intercept of the plotting log K b versus 1/ T curve, respectively (Figure ) (Table ).…”

Section: Resultsmentioning

confidence: 99%

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Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

Sabziparvar¹,

Saeedi²,

Nouri³

et al. 2018

J. Phys. Chem. B

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Nanoparticles (NPs) have received great attention in biological and medical applications because of their unique features. However, their induced adverse effects on the biological system are not well-explored. Herein, the interaction of silicon dioxide nanoparticles (SiO NPs) with human hemoglobin (Hb) and lymphocyte cell line was evaluated under physiological conditions by multispectroscopic [intrinsic and synchronous fluorescence spectroscopy and circular dichrosim (CD)], molecular docking, and cellular [3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) and acridine orange/ethidium bromide (AO/EB) staining] methods. Transmission electron microscopy and dynamic light scattering revealed the nanosized and spherical shaped SiO particle. The fluorescence and lifetime decay results indicated that SiO NPs quenched the intrinsic intensity of Hb through a static quenching mechanism. The binding affinity of SiO NPs toward Hb was directly correlated with temperature. The sign of thermodynamic parameters demonstrated that hydrophobic forces played a pivotal role in the interaction of SiO NPs with Hb. The results of synchronous fluorescence experiments displayed that Tyr residues are moved to a more hydrophilic microenvironment. Molecular docking studies exhibited that SiO and Si NPs were bound to Hb primarily by hydrophobic residues. The findings from CD data verified no alteration in the secondary structure of Hb upon binding to SiO NPs. The human lymphocyte cell line was treated with SiO NPs at varying concentrations and time intervals and the cytotoxicity assays by MTT and AO/EB staining showed that cell viability was reduced by the SiO NP-induced apoptosis mechanism in a dose and time-dependent manner. Therefore, it may be suggested that comprehensive details regarding the interaction of NPs and biological systems such as cells and proteins can provide useful information in the development of NP-based systems.

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Section: Resultsmentioning

confidence: 99%

“…The impact of binding forces in NP−protein interactions is pivotal. Several forces play The values of ΔH°and ΔS°were calculated using the van't Hoff equation 35…”

Section: The Journal Of Physical Chemistry Bmentioning

confidence: 99%

Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

Sabziparvar¹,

Saeedi²,

Nouri³

et al. 2018

J. Phys. Chem. B

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“…FTIR spectra of the ZnS quantum dots (Figure 2) confirm the interactions of the octadecylamine-capping agent with ZnS. The C-C stretching vibrations of the capping agent appeared at 2853 cm −1 and 2907 cm −1 , while the bands at 1367 cm −1 and 1466 cm −1 are attributed to C-H stretching vibrational modes [39]. ZnS stretching vibrations were observed at 1636 cm −1 and 1089 cm −1 [40][41][42], while the N-H absorption band was observed at 3330 cm −1 , which confirms octadecylamine (ODA) as a capping agent on the surface of the ZnS quantum dots.…”

Section: Resultsmentioning

confidence: 71%

Photocatalytic Degradation of Single and Binary Mixture of Brilliant Green and Rhodamine B Dyes by Zinc Sulfide Quantum Dots

Ajibade

Oluwalana

2021

Molecules

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We present the preparation of octadecylamine-capped ZnS quantum dots from bis(morpholinyldithiocarbamato)Zn(II) complex. The complex was thermolyzed at 130 °C in octadecylamine at different times, to study the effect of reaction time on the morphological and photocatalytic properties of the ZnS quantum dots. Powder X-ray diffraction patterns confirmed a hexagonal wurtzite crystalline phase of ZnS, while HRTEM images showed particle sizes of about 1–3 nm, and energy band gaps of 3.68 eV (ZnS–1), 3.87 eV (ZnS–2), and 4.16 eV (ZnS–3) were obtained from the Tauc plot for the ZnS nanoparticles. The as-prepared ZnS were used as photocatalysts for the degradation of brilliant green, rhodamine B, and binary dye consisting of a mixture of brilliant green-rhodamine B. The highest photocatalytic degradation efficiency of 94% was obtained from ZnS–3 with low photoluminescence intensity. The effect of catalytic dosage and pH of the dyes solution on the photocatalytic process shows that pH 8 is optimal for the degradation of brilliant green, while pH 6.5 is the best for photocatalytic degradation of rhodamine B. The degradation of the binary dyes followed the same trends. The effect of catalytic dosage shows that 1 mg mL−1 of the ZnS nano-photocatalyst is the optimum dosage for the degradation of organic dyes. Reusability studies show that the ZnS quantum dots can be reused five times without a significant reduction in degradation efficiency.

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“…Similarly, Pradhan et al have shown that iron oxide nanoparticles conjugated with glutamine- and proline-based osmolytes inhibit protein aggregation [24]. Hosseinzadeh et al showed that the interaction of quantum dots (QDs) and proteins strongly influenced the surface characteristics of the QDs at the protein-QD interface [25]. A recent review describes the biosensing ability of natural receptor-conjugated conducting nanomaterials [26].…”

Section: Introductionmentioning

confidence: 99%

Conjugation of Carbon Dots with β-Galactosidase Enzyme: Surface Chemistry and Use in Biosensing

Sharma

Mićić

et al. 2019

Molecules

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Nanoparticles have been conjugated to biological systems for numerous applications such as self-assembly, sensing, imaging, and therapy. Development of more reliable and robust biosensors that exhibit high response rate, increased detection limit, and enhanced useful lifetime is in high demand. We have developed a sensing platform by the conjugation of β-galactosidase, a crucial enzyme, with lab-synthesized gel-like carbon dots (CDs) which have high luminescence, photostability, and easy surface functionalization. We found that the conjugated enzyme exhibited higher stability towards temperature and pH changes in comparison to the native enzyme. This enriched property of the enzyme was distinctly used to develop a stable, reliable, robust biosensor. The detection limit of the biosensor was found to be 2.9 × 10−4 M, whereas its sensitivity was 0.81 µA·mmol−1·cm−2. Further, we used the Langmuir monolayer technique to understand the surface properties of the conjugated enzyme. It was found that the conjugate was highly stable at the air/subphase interface which additionally reinforces the suitability of the use of the conjugated enzyme for the biosensing applications.

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Interaction of insulin with colloidal ZnS quantum dots functionalized by various surface capping agents

Cited by 14 publications

References 61 publications

Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

Photocatalytic Degradation of Single and Binary Mixture of Brilliant Green and Rhodamine B Dyes by Zinc Sulfide Quantum Dots

Conjugation of Carbon Dots with β-Galactosidase Enzyme: Surface Chemistry and Use in Biosensing

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