Interaction of Iron Oxide Fe3O4 Nanoparticles and Alveolar Macrophages in Vivo

Katsnelson, Boris A.; Privalova, Larisa I.; Sutunkova, Marina P.; Tulakina, Ludmila G.; Pichugova, S. V.; Beykin, J. B.; Khodos, M. J.

doi:10.1007/s10517-012-1593-z

Cited by 19 publications

(16 citation statements)

References 6 publications

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“…However, it is impossible to judge whether the latter had penetrated into the cells in preformed state or had formed inside merged phagosomes. (The second mechanism seemed more likely for 10 nm particles of iron oxide, judging by the pattern of their distribution inside AM [4,5]).…”

Section: Resultsmentioning

confidence: 99%

Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors

Katsnelson

Privalova

Gurvich

et al. 2013

IJMS

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Stable suspensions of nanogold (NG) and nanosilver (NS) with mean particle diameter 50 and 49 nm, respectively, were prepared by laser ablation of metals in water. To assess rat’s pulmonary phagocytosis response to a single intratracheal instillation of these suspensions, we used optical, transmission electron, and semi-contact atomic force microscopy. NG and NS were also repeatedly injected intraperitoneally into rats at a dose of 10 mg/kg (0.5 mg per mL of deionized water) three times a week, up to 20 injections. A group of rats was thus injected with NS after oral administration of a “bioprotective complex” (BPC) comprised of pectin, multivitamins, some amino acids, calcium, selenium, and omega-3 PUFA. After the termination of the injections, many functional and biochemical indices and histopathological features of the spleen, kidneys and liver were evaluated for signs of toxicity, and accumulation of NG or NS in these organs was measured. From the same rats, we obtained cell suspensions of different tissues for performing the RAPD test. It was demonstrated that, although both nanometals were adversely bioactive in all respects considered in this study, NS was more noxious as compared with NG, and that the BPC tested by us attenuated both the toxicity and genotoxicity of NS.

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Section: Resultsmentioning

confidence: 99%

Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors

Katsnelson

Privalova

Gurvich

et al. 2013

IJMS

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“…[1][2][3][4][5][6][7][8][9][10] We experimented with NPs of iron oxide Fe 3 O 4 (magnetite) having mean diameters 10 nm, 50 nm or 1 mcm, gold (4 nm or 50 nm), silver (4 nm, 49 nm or 1.1 mcm), copper oxide (20 nm or 340 nm), nickel oxide NiO (30 nm), and manganese oxide Mn 3 O 4 (32 nm). Keeping in mind the above mentioned theoretical premises of our research, we used purposefully prepared and accurately characterized NPs of pure metals or their oxides suspended in deionized water rather than commercial nanomaterials.…”

Section: Methodsmentioning

confidence: 99%

“…Indeed, although the mean diameter The TEM images ( Figure 8) also testify to the physiological endocytosis of NPs as opposed to their direct penetration through cell membrane by diffusion, which, above any doubts, is also possible. [4][5][6] When NPs are of given dimensions, the pulmonary phagocytosis response strongly depends on their chemical nature. For instance, as can be seen from Table 2, the total cell, NL and AM counts, and the macrophage count of the BALF increased under the impact of silver NPs more than it did under the impact of virtually equidimensional gold NPs.…”

Section: Some Inferences From In Vivo Experiments With Metallic Nanopmentioning

confidence: 99%

“…During 2009-2014 our experimental work was focused on trying to find answers to these important questions. [1][2][3][4][5][6][7][8][9][10] Although in the vast nanotoxicological literature of the last decade studies concerned with the assessment of metal or metal-oxide NP toxicity are not at the top of the list, such publications are nevertheless quite numerous. To illustrate this, we may refer to several works devoted to NPs of the metals that were the subject-matter of our own studies considered below: iron oxide, [11][12][13][14][15][16][17][18][19][20] silver, gold, 24,[45][46][47][48][49][50][51][52][53][54][55][56][57][58] copper and copper oxide, 43,[59][60][61][62][63][64][65][66][67][68] nickel oxide, 65,…”

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confidence: 99%

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Some inferences from in vivo experiments with metal and metal oxide nanoparticles: the pulmonary phagocytosis response, subchronic systemic toxicity and genotoxicity, regulatory proposals, searching for bioprotectors (a self-overview)

Katsnelson¹,

Privalova²,

Sutunkova³

et al. 2015

IJN

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The purpose of this paper is to overview and summarize previously published results of our experiments on white rats exposed to either a single intratracheal instillation or repeated intraperitoneal injections of silver, gold, iron oxide, copper oxide, nickel oxide, and manganese oxide nanoparticles (NPs) in stable water suspensions without any chemical additives. Based on these results and some corroborating data of other researchers we maintain that these NPs are much more noxious on both cellular and systemic levels as compared with their 1 μm or even submicron counterparts. However, within the nanometer range the dependence of systemic toxicity on particle size is intricate and non-unique due to complex and often contra-directional relationships between the intrinsic biological aggressiveness of the specific NPs, on the one hand, and complex mechanisms that control their biokinetics, on the other. Our data testify to the high activity of the pulmonary phagocytosis of NPs deposited in airways. This fact suggests that safe levels of exposure to airborne NPs are possible in principle. However, there are no reliable foundations for establishing different permissible exposure levels for particles of different size within the nanometric range. For workroom air, such permissible exposure levels of metallic NP can be proposed at this stage, even if tentatively, based on a sufficiently conservative approach of decreasing approximately tenfold the exposure limits officially established for respective micro-scale industrial aerosols. It was shown that against the background of adequately composed combinations of some bioactive agents (comprising pectin, multivitamin-multimineral preparations, some amino acids, and omega-3 polyunsaturated fatty acid) the systemic toxicity and even genotoxicity of metallic NPs could be markedly attenuated. Therefore we believe that, along with decreasing NP-exposures, enhancing organisms’ resistance to their adverse action with the help of such bioprotectors can prove an efficient auxiliary tool of health risk management in occupations connected with them.

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“…Thus, engineered nanoparticles (NPs) of this kind are of interest for industrial toxicology not only as such, but also as a model object for characterizing the harmful action of the nano-fractions of corresponding industrial aerosols on the organism. Based on our studies of supermagnetic iron oxide (II, III), silver, and gold nanoparticles [1,2,3,4,5,6,7,8] which are actually encountered only in the manufacture of corresponding nanomaterials and in their applications in science, technology, or medicine, we proceeded to experimental research into the here stated problem, starting with copper oxide nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Subchronic Toxicity of Copper Oxide Nanoparticles and Its Attenuation with the Help of a Combination of Bioprotectors

Privalova

Katsnelson

Loginova

et al. 2014

IJMS

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In the copper metallurgy workplace air is polluted with condensation aerosols, which a significant fraction of is presented by copper oxide particles <100 nm. In the scientific literature, there is a lack of their in vivo toxicity characterization and virtually no attempts of enhancing organism’s resistance to their impact. A stable suspension of copper oxide particles with mean (±SD) diameter 20 ± 10 nm was prepared by laser ablation of pure copper in water. It was being injected intraperitoneally to rats at a dose of 10 mg/kg (0.5 mg per mL of deionized water) three times a week up to 19 injections. In parallel, another group of rats was so injected with the same suspension against the background of oral administration of a “bio-protective complex” (BPC) comprising pectin, a multivitamin-multimineral preparation, some amino acids and fish oil rich in ω-3 PUFA. After the termination of injections, many functional and biochemical indices for the organism’s status, as well as pathological changes of liver, spleen, kidneys, and brain microscopic structure were evaluated for signs of toxicity. In the same organs we have measured accumulation of copper while their cells were used for performing the Random Amplification of Polymorphic DNA (RAPD) test for DNA fragmentation. The same features were assessed in control rats infected intraperitoneally with water with or without administration of the BPC. The copper oxide nanoparticles proved adversely bio-active in all respects considered in this study, their active in vivo solubilization in biological fluids playing presumably an important role in both toxicokinetics and toxicodynamics. The BPC proposed and tested by us attenuated systemic and target organs toxicity, as well as genotoxicity of this substance. Judging by experimental data obtained in this investigation, occupational exposures to nano-scale copper oxide particles can present a significant health risk while the further search for its management with the help of innocuous bioprotectors seems to be justified.

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Interaction of Iron Oxide Fe3O4 Nanoparticles and Alveolar Macrophages in Vivo

Cited by 19 publications

References 6 publications

Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors

Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors

Some inferences from in vivo experiments with metal and metal oxide nanoparticles: the pulmonary phagocytosis response, subchronic systemic toxicity and genotoxicity, regulatory proposals, searching for bioprotectors (a self-overview)

Subchronic Toxicity of Copper Oxide Nanoparticles and Its Attenuation with the Help of a Combination of Bioprotectors

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