Interaction of L antibody with low potassium-type sheep red cells: Resolution of two separate functional antibodies

Smalley, C. E.; Tucker, Elizabeth M.; Dunham, Philip B.; Ellory, J. C.

doi:10.1007/bf01870882

Cited by 18 publications

(14 citation statements)

References 23 publications

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“…The absorbed serum contained anti-Lb; adsorbed anti-Lb was then eluted from the trypsinized cells using an isotonic medium at pH 3 3 containing glycine-HCl. This method will be described in detail elsewhere (Smalley, Tucker, Dunham & Ellory, 1981).…”

Section: Introductionmentioning

confidence: 99%

Passive potassium transport in low potassium sheep red cells: dependence upon cell volume and chloride.

Dunham¹,

Ellory²

1981

The Journal of Physiology

241

168

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The major pathway of passive K influx (ouabain-insensitive) was characterized in low-K type (LK) red cells of sheep. 1. Passive K transport in these cells was highly sensitive to variations in cell volume; it increased threefold or more in cells swollen osmotically by 10%, and decreased up to twofold in cells shrunken 5-10%. Active K influx was insensitive to changes in cell volume. Three different methods for varying cell volume osmotically all gave similar results. 2. The volume-sensitive pathway was specific for K in that Na influx did not vary with changes in cell volume. 3. The volume-sensitive K influx was a saturable function of external K concentration. It was slightly inhibited by Na, whereas K influx in shrunken cells was unaffected by Na. 4. Passive K influx was dependent on the major anion in the medium in that replacement of Cl with any of six other anions resulted in a reduction of K influx by 50-80% (replacement of Cl by Br caused an increase in K influx). The activation of K influx by Cl followed sigmoid kinetics. 5. Passive K influx is inhibited by anti-L antibody. The antibody affected only that portion of influx which was Cl-dependent and volume-sensitve. Of the subfractions of the antibody, it is anti-L1 which inhibits passive K transport. 6. Pretreatment of cells with iodoacetamide reduced the sensitivity of K influx to cell volume in that the influx was reduced in swollen IAA-treated cells and increased in shrunken IAA-cells. 7. Intracellular Ca has no role in altering passive K transport in LK sheep cells. Therefore, the major pathway of passive K transport in LK sheep red cells is sensitive to changes in cell volume, specific for K, dependent on Cl, and inhibited by anti-L1 antibody, The minor pathway, observed in shrunken cells, has none of these properties.

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Section: Introductionmentioning

confidence: 99%

Passive potassium transport in low potassium sheep red cells: dependence upon cell volume and chloride.

Dunham¹,

Ellory²

1981

The Journal of Physiology

241

168

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“…A very high furosemide or bumetanide concentration (about 2 mM) is required for 50% inhibition [63]. The K + flux in LK sheep red cells is inhibited by anti-L 1 , a specific component of an antiserum against LK sheep red cells [297]. The K + flux must involve a small number of transport sites, since there are only about 850 anti-L binding sites per cell [297], or else the effect of anti-L 1 must be highly cooperative or mediated through a second messenger.…”

Section: Sheep Red Cellsmentioning

confidence: 98%

“…The K + flux in LK sheep red cells is inhibited by anti-L 1 , a specific component of an antiserum against LK sheep red cells [297]. The K + flux must involve a small number of transport sites, since there are only about 850 anti-L binding sites per cell [297], or else the effect of anti-L 1 must be highly cooperative or mediated through a second messenger. The number of cot ran sport sites may even be an order of magnitude lower than the number of antigen sites [297].…”

Section: Sheep Red Cellsmentioning

confidence: 99%

“…The K + flux must involve a small number of transport sites, since there are only about 850 anti-L binding sites per cell [297], or else the effect of anti-L 1 must be highly cooperative or mediated through a second messenger. The number of cot ran sport sites may even be an order of magnitude lower than the number of antigen sites [297]. Compression at high hydrostatic pressure also induces a large Cl--dependent K + flux in LK sheep red cells which shows many of the characteristics of the hypotonically induced flux [64,65].…”

Section: Sheep Red Cellsmentioning

confidence: 99%

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Membrane Mechanisms in Volume Regulation in Vertebrate Cells and Epithelia

Hoffmann¹,

Ussing²

1992

Membrane Transport in Biology

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“…Results consistently show L activity associated with a 25 kDa polypeptide in LK red cells. Anti-L alloantisera has been divided into two activities; pump-stimulating activity (termed Lp) and KCl cotransport-inhibiting activity (termed Ll), this latter activity also showing strong complement binding (Smalley et al 1982). By sensitizing LK cells with an anti-L known to have strong Lp, but weak Ll, activity we have demonstrated that the 25 kDa polypeptide is recognized by the pump-stimulating Lp antiserum and therefore conclude that this glycoprotein is involved in sodium pump inhibition in LK sheep red cells.…”

Section: P Bristol Meeting 9-10 Februar Y 1990mentioning

confidence: 99%

Proceedings of the Physiological Society, 9‐10 February 1990, Bristol Meeting: Communications

Suidan

Murrell

Tolkovsky

1990

The Journal of Physiology

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Association between inhibition of a noxious viscerosomatic reflex and cardiovascular changes evoked by stimulation in the rostral hypothalamus in anaesthetized rats BY T. A. LOVICK and B. M. LUMB. Departments of Physiology, University of Birmingham, Birmingham B15 2TJ and University of Bristol, Bristol BS8 lTD It is a common experience that under certain extreme conditions, for example during combat or competitive sport, responsiveness to pain may be reduced. An association between analgesia and the specific pattern of cardiovascular changes which is characteristic of attack/defensive-type behaviour has previously been described in response to stimulation in the dorsal midbrain (Duggan & Morton, 1984; Lovick, 1985). More recently it has been shown that stimulation of neuronal cell bodies in the anterior hypothalamus/preoptic area of the forebrain can inhibit spinal processing of noxious visceral afferent input (Lumb, 1989; Lumb & Cervero, 1989). This area of the forebrain is also known to be involved in the control of cardiovascular responsiveness. Therefore in the present study we have investigated the possibility that antinociception evoked from the ventral forebrain might be accompanied by a specific pattern of cardiovascular response.Experiments were carried out on 10 male rats anaesthetized with alphaxalone/ alphadolone (9-12 mg/kg per h, i.v.). They were instrumented to record blood pressure, heart rate, hindlimb muscle blood flow and respiration. Reflex activity in spinal nerves LI or L2 was recorded in the response to electrical stimulation of visceral afferent nerve fibres in the splanchnic nerve at 1-5 x threshold intensity. Glass micropipettes containing 0-2 M-D,L-homocysteic acid (DLH, pH 7-5) were positioned in the ventromedial forebrain for selective activation of neuronal cell bodies.Microinjection of DLH produced a clear-cut inhibition of the reflex at 7 out of 65 sites. The effective sites were located in the anterior hypothalamus/preoptic area (AH/PO). Injections made rostral or caudal to this region failed to produce any change in reflex activity. At all 7 positive injection sites inhibition of the reflex was accompanied by an increase in blood pressure, tachycardia, vasodilatation in hindlimb muscle and tachypnoea. This pattern of response was never evoked by injections made at other sites. Indeed, at sites where DLH failed to affect reflex activity there was either a fall in blood pressure and heart rate and vasodilatation in the hindlimb or no change in the cardiovascular parameters measured.We suggest that the AH/PO may be involved in integrating the various components of the whole response of the animal to stimuli which evoke defensive/attack-type behaviour and inhibition of noxious afferent input from the viscera.

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Interaction of L antibody with low potassium-type sheep red cells: Resolution of two separate functional antibodies

Cited by 18 publications

References 23 publications

Passive potassium transport in low potassium sheep red cells: dependence upon cell volume and chloride.

Passive potassium transport in low potassium sheep red cells: dependence upon cell volume and chloride.

Membrane Mechanisms in Volume Regulation in Vertebrate Cells and Epithelia

Proceedings of the Physiological Society, 9‐10 February 1990, Bristol Meeting: Communications

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