Interaction of Lipid Monolayers with Aqueous Neutral Polymers and the Consequent Monolayer Stabilization and Improved Langmuir-Blodgett Transfer

Ariga, Katsuhiko; Shin, Jae Sup; Kunitake, Toyoki

doi:10.1006/jcis.1995.1123

Cited by 21 publications

(22 citation statements)

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“…Similarly, we expect that PEG interacts only minimally with glass or quartz surfaces. For example, soluble PEG cannot be transferred from the subphase under a compressed lipid monolayer (Ariga et al, 1995). Based on these favorable observations, we designed a new PEG-phospholipid conjugate that could be covalently bonded to silicate substrates.…”

Section: Introductionmentioning

confidence: 99%

Tethered Polymer-Supported Planar Lipid Bilayers for Reconstitution of Integral Membrane Proteins: Silane-Polyethyleneglycol-Lipid as a Cushion and Covalent Linker

Wagner

Tamm

2000

Biophysical Journal

513

464

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There is increasing interest in supported membranes as models of biological membranes and as a physiological matrix for studying the structure and function of membrane proteins and receptors. A common problem of protein-lipid bilayers that are directly supported on a hydrophilic substrate is nonphysiological interactions of integral membrane proteins with the solid support to the extent that they will not diffuse in the plane of the membrane. To alleviate some of these problems we have developed a new tethered polymer-supported planar lipid bilayer system, which permitted us to reconstitute integral membrane proteins in a laterally mobile form. We have supported lipid bilayers on a newly designed polyethyleneglycol cushion, which provided a soft support and, for increased stability, covalent linkage of the membranes to the supporting quartz or glass substrates. The formation and morphology of the bilayers were followed by total internal reflection and epifluorescence microscopy, and the lateral diffusion of the lipids and proteins in the bilayer was monitored by fluorescence recovery after photobleaching. Uniform bilayers with high lateral lipid diffusion coefficients (0.8-1.2 x 10(-8) cm(2)/s) were observed when the polymer concentration was kept slightly below the mushroom-to-brush transition. Cytochrome b(5) and annexin V were used as first test proteins in this system. When reconstituted in supported bilayers that were directly supported on quartz, both proteins were largely immobile with mobile fractions < 25%. However, two populations of laterally mobile proteins were observed in the polymer-supported bilayers. Approximately 25% of cytochrome b(5) diffused with a diffusion coefficient of approximately 1 x 10(-8) cm(2)/s, and 50-60% diffused with a diffusion coefficient of approximately 2 x 10(-10) cm(2)/s. Similarly, one-third of annexin V diffused with a diffusion coefficient of approximately 3 x 10(-9) cm(2)/s, and two-thirds diffused with a diffusion coefficient of approximately 4 x 10(-10) cm(2)/s. A model for the interaction of these proteins with the underlying polymer is discussed.

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Section: Introductionmentioning

confidence: 99%

Tethered Polymer-Supported Planar Lipid Bilayers for Reconstitution of Integral Membrane Proteins: Silane-Polyethyleneglycol-Lipid as a Cushion and Covalent Linker

Wagner

Tamm

2000

Biophysical Journal

513

464

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“…[1][2][3] Langmuir monolayer at the air-water interface plays an important role in the application of Langmuir-Blodgett (LB) technique. [4][5][6] The behavior of Langmuir monolayer at the air-water interface is of great importance for understanding the structure, stability, and deposition transfer ratio of the monolayer. By measuring and analyzing the pressure versus area isotherm, one would be able to understand the states of the monolayer and its changes, for example, molecular arrangement, phase structure, phase transition, etc.…”

Section: Introductionmentioning

confidence: 99%

Aggregation of P-Terphenyl Along With Pmma/Sa at the Langmuir and Langmuir–blodgett Films

et al. 2008

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Molecular aggregation and monolayer characteristics of non-amphiphilic p-terphenyl (TP) mixed with either polymethyl methacrylate (PMMA) or stearic acid (SA) at the air-water interface were investigated. The miscibility of the two components was evaluated by measuring and analyzing surface pressure versus area per molecule (π-A) isotherm. Both attractive and repulsive interactions between the sample (TP) and the matrix (PMMA or SA) were observed depending on the composition and microenvironment. TP and PMMA/SA were not completely miscible in the mixed monolayer. Aggregation and phase separation between sample TP and matrix molecule were revealed by UV-Vis absorption spectroscopic studies and confirmed by scanning electron micrograph of Langmuir-Blodgett films.

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“…This suggests that the attachment of DNA to DPPC increases the effective molecular area, and during the compression, the molecules start to interact with each other via the attached DNA strands at much longer distance than they would without DNA. The interaction occurring in the expanded region of the isotherm is in part similar to that of lipid monolayers in nonspecifical interaction with neutral polymers, 14 however, the behavior in the compressed region is different, as the isotherm in the presence of DNA is shifted toward larger area per molecule. We can also make a comparison with the interaction of myelin basic protein (MBP), a positively charged, intrinsically unstructured protein, with different phospholipid monolayers.…”

Section: Resultsmentioning

confidence: 91%

Structural Study of the DNA Dipalmitoylphosphatidylcholine Complex at the Air−Water Interface

et al. 2007

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We present here results that demonstrate the formation of a complex of DNA with zwitterionic dipalmitoylphosphatidylcholine (DPPC) monolayer at the air-water interface in the presence of Ca2+ ions; in particular, we show that the presence of Ca2+ cations is essential for the formation of the complex of DPPC with DNA. We characterize the resulting structure by X-ray reflectivity and by null-ellipsometry. We show that DNA maintains its native double helix form when attached to the zwitterionic lipid monolayer, at difference with the case of ammine containing monolayers. Our findings are discussed in view of other works that recently appeared on the interaction of DNA with zwitterionic phospholipids, emphasizing the role of DPPC as a potential vector for transfer of genetic material into mammalian cells by nonviral gene therapy and also suggesting Langmuir/Blodgett layers of zwitterionic phospoholipids as a method for nonconventional DNA immobilization.

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Interaction of Lipid Monolayers with Aqueous Neutral Polymers and the Consequent Monolayer Stabilization and Improved Langmuir-Blodgett Transfer

Cited by 21 publications

References 0 publications

Tethered Polymer-Supported Planar Lipid Bilayers for Reconstitution of Integral Membrane Proteins: Silane-Polyethyleneglycol-Lipid as a Cushion and Covalent Linker

Tethered Polymer-Supported Planar Lipid Bilayers for Reconstitution of Integral Membrane Proteins: Silane-Polyethyleneglycol-Lipid as a Cushion and Covalent Linker

Aggregation of P-Terphenyl Along With Pmma/Sa at the Langmuir and Langmuir–blodgett Films

Structural Study of the DNA Dipalmitoylphosphatidylcholine Complex at the Air−Water Interface

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