Interaction of liposomal and polycationic transfection complexes with pulmonary surfactant

Ernst, Nicola; Ulrichskötter, Sonja; Schmalix, Wolfgang A.; Rädler, Joachim O.; Galneder, Reinhard; Mayer, Elfriede; Gersting, Søren W.; Plank, Christian; Reinhardt, D.; Rosenecker, Joseph

doi:10.1002/(sici)1521-2254(199909/10)1:5<331::aid-jgm60>3.0.co;2-8

Cited by 79 publications

(64 citation statements)

References 14 publications

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“…These data confirm for the first time in vivo, the previous in vitro findings of the inhibition of cationic lipid-mediated gene transfer by natural and synthetic surfactant preparations. 18,19 The lack of inhibition by surfactant of cationic polymer-mediated gene transfer is similar to what has been seen using adenoviral vectors. Indeed, it has been shown that surfactant facilitates adenovirally mediated gene transfer in the peripheral lung and enhances transgene expression.…”

Section: Figure 5 Immunohistochemical Detection Of Gfp Transgene Exprmentioning

confidence: 56%

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Biodistribution and transgene expression with nonviral cationic vector/DNA complexes in the lungs

Bragonzi¹,

et al. 2000

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Biodistribution of nonviral cationic vector/DNA complexes was studied after systemic or intratracheal administration to the lungs and correlated with transgene expression. Intravenous injection in C57Bl/6 mice gave maximal and significant luciferase expression in the lungs with the cationic polymer PEI 22K/DNA complexes at the highest ratios of positive/negative charges versus DNA alone. While DOTAP/DNA complexes with high charge ratio determined lower but still significant luciferase activity versus uncomplexed DNA, GL-67A and PEI 25K mediated negligible luciferase expression. Labelled PEI 22K and DOTAP complexes were evenly distributed in the alveolar region, where GFP expression was revealed, while PEI 25K and GL-67A complexes were not detected, suggesting a different interaction of these complexes with the plasma membrane of endothelial cells. Following an intratracheal injection, the highest and significant levels of transfection were obtained with

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Section: Figure 5 Immunohistochemical Detection Of Gfp Transgene Exprmentioning

confidence: 56%

“…In vitro studies have demonstrated that natural and synthetic preparations of surfactant markedly inhibited gene transfer mediated by cationic liposome, 18 but not by PEI and dendrimers. 19 However, the role of surfactant has not been studied in vivo.…”

Section: Introductionmentioning

confidence: 99%

Biodistribution and transgene expression with nonviral cationic vector/DNA complexes in the lungs

Bragonzi¹,

et al. 2000

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“…8,22,23 Others have reported that Alveofact, a natural pulmonary surfactant, forms a barrier to cationic lipid-mediated gene transfer. [11][12][13] We examined whether this also held true for our lipoplexes.…”

Section: Does Alveofact Influence Gene Expression Zeta Potential Andmentioning

confidence: 92%

“…Only a few papers have shown that pulmonary surfactants, which line the alveoli, decrease the gene transfer activity of cationic liposomes. [11][12][13] However, these authors did not elucidate in detail the underlying mechanism for this inhibition in activity.…”

Section: Introductionmentioning

confidence: 99%

Pegylated GL67 lipoplexes retain their gene transfection activity after exposure to components of CF mucus

Sanders

Smedt

Cheng³

et al. 2002

Gene Ther

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“…Hence, the impact of pulmonary surfactant on the stability and transfection efficiency of specific nucleic acid nanocarriers has been evaluated in the past [14][15][16]. A general finding of these studies is the incompatibility of cationic lipid based nanocarriers with pulmonary surfactants.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Natural Pulmonary Surfactant on The Efficacy of siRNA-Loaded Dextran Nanogels

et al. 2013

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Aims: Topical administration of small interfering RNA (siRNA) nanocarriers is a promising approach in the treatment of pulmonary disorders. Pulmonary surfactant, covering the entire alveolar surface of mammalian lungs, will be one of the first interfaces that siRNA nanocarriers encounter upon inhalation therapy. Therefore, it is of outstanding importance to evaluate the impact of pulmonary surfactant on the performance of siRNA nanocarriers. Materials & Methods: The effect of natural lung-derived surfactants on the siRNA delivery capacity of dextran nanogels (DEX-NGs) is evaluated in vitro using flow cytometry and confocal microscopy.

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Interaction of liposomal and polycationic transfection complexes with pulmonary surfactant

Cited by 79 publications

References 14 publications

Biodistribution and transgene expression with nonviral cationic vector/DNA complexes in the lungs

Biodistribution and transgene expression with nonviral cationic vector/DNA complexes in the lungs

Pegylated GL67 lipoplexes retain their gene transfection activity after exposure to components of CF mucus

The Influence of Natural Pulmonary Surfactant on The Efficacy of siRNA-Loaded Dextran Nanogels

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