Interaction of Low-Intensity Laser Radiation with Blood and Its Components

Abstract

The response of the blood of rabbits to the intravenous irradiation by a He-Ne laser radiation (λ = 632.8 nm) has been investigated by the UV-visible and IR absorption spectra of the whole blood, plasma, and erythrocyte mass. It has been established that hemoglobin is a primary photoacceptor absorbing low-intensity HeNe-laser radiation. The exposure of blood to this radiation causes clearly defined changes in the IR and visible absorption spectra of the blood and erythrocytes. These spectral changes arise as a result of partial photodissociation of hemoglobin-ligand complexes in the process of absorption of laser radiation. It is suggested that photodissociation is a primary reaction that arises in blood exposed to a low-intensity laser radiation.Keywords: low-intensity laser radiation, intravenous irradiation of blood, hemoglobin, UV-visible and IR spectra of blood, hemoglobin photodissociation.Introduction. At present, low-intensity laser radiation is widely used in clinical practice. The intravenous irradiation of blood is considered as the most efficient method of biostimulation of an organism for therapeutic purposes. The effects, including the therapeutic effect, arising in living organisms exposed to laser radiation for the purpose of treatment of various diseases have been described in detail in the literature [1,2]. The ability of living biosystems to transform their metabolism under the action of low-intensity laser radiation was revealed for the first time from the spectral dependences of the photosensitivity of various bioprocesses to laser radiation [3][4][5]. However, up to now there has been no unique theory developed to explain the mechanism of action of a low-intensity laser radiation on even the simplest bioobjects, such as cells and biocultures, and the effect arising in this case [4,6,7]. Therefore, the study of the mechanism of interaction of a low-intensity laser radiation with living organisms by various methods for the purpose of widening the field of its medical application is undoubtedly a pressing problems. The solution of this problem will aid in the further development of practical medicine. Despite the fact that the response of blood to the action of a low-intensity laser radiation gives important information on the mechanism of interaction of laser radiation with a living organism [8,9], only a small number of works have been devoted to such investigations in living organisms. In the present work, we carried out spectral investigations of the response of the blood of experimental animals (rabbits) to the intravenous irradiation by laser radiation. The changes in the spectral characteristics of the blood exposed to a low-intensity laser radiation were analyzed using the IR (range 4-15 µm) and UV-visible (range 200-800 nm) absorption spectra of the whole blood, plasma, and erythrocyte mass.Experimental Procedure. Samples of the whole venous blood of experimental animals (rabbits) taken before and after the intravenous irradiation of them by a low-intensity He-Ne laser (HNL) radiatio...

Optical methods for correction of oxygen-transport characteristics of blood and their biomedical applications

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Determination of the concentration of cephalosporin antibiotics in blood by infrared spectroscopy

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Enhancement of Human Blood Storage Period by Irradiation of Low Level He-Ne Laser

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