Interaction of Lymphoid and Nonlymphoid Cells with the Lymphocytosis-Promoting Factor of
            <i>Bordetella pertussis</i>

Adler, Ava; Morse, Stephen I.

doi:10.1128/iai.7.3.461-467.1973

Cited by 12 publications

(4 citation statements)

References 6 publications

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“…1B7 may detach more easily from the cell-PT-MAb complex by being washed compared with the effects for 11E6. The results of cell infusion experiments suggest that PT interacts reversibly or successively with target cells in vivo as reported previously (1,3,22) and also suggest that the epitope for 1B7 may not be related to the initial binding site of the cell. As four distinct MAbs were characterized in this study, they can be used to analyze the complex process of PT activity in vivo by an in vitro system.…”

Section: Discussionsupporting

confidence: 79%

Effect of monoclonal antibody to pertussis toxin on toxin activity

Sato¹,

Sato²,

Ito³

et al. 1987

Infect Immun

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Two distinct monoclonal antibodies, one to pertussis toxin subunit S2, called 9G8, and another to subunits S2 and S3, called 11E6, were generated from the hybridomas of myeloma SP2/0 and spleen cells of BALB/c mice immunized mainly with the subunit S234 complex. Binding ability of 9G8 and 11E6 to the subunits was confirmed by the enzyme-linked immunosorbent assay and immunoblotting analysis. Generation of 11E6 bound to both S2 and S3 might mean that there is common antigenicity between S2 and S3. Neutralizing activities of 9G8 and 11E6 on various biological activities of pertussis toxin, including ADP-ribosyltransferase and leukocytosis-promoting, islet-activating, permeability-increasing. Chinese hamster ovary (CHO) cell-clustering, and hemagglutinating activities, were compared with those of anti-S1 monoclonal antibodies 1B7 and 3F10, which were isolated and characterized in a previous study (H. Sato, A. Ito, J. Chiba, and Y. Sato, Infect. Immun. 46:422-428, 1984). 1B7 and 3F10 neutralized ADP-ribosyltransferase activity of pertussis toxin or S1, but 9G8 and 11E6 did not. 1B7 showed very potent neutralization against leukocytosis-promoting, islet-activating, permeability-increasing, and CHO cell-clustering activities of pertussis toxin, but 3F10 did not, although anti-ADP-ribosyltransferase activities of both antibodies were identical. 11E6 neutralized leukocytosis-promoting, islet-activating, CHO cell-clustering, and hemagglutinating activities but not permeability-increasing activity. 9G8 showed slight neutralization of leukocytosis-promoting and CHO cell-clustering activities. Specific activities of 1B7 and 11E6 in each neutralization test were higher than or almost comparable to those of polyclonal antibodies to pertussis toxin. The neutralizing mechanism of 1B7 and 11E6 in leukocytosis-promoting activity was compared. 11E6 seemed to interfere with the binding of pertussis toxin to receptors on mouse spleen cells.

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Section: Discussionsupporting

confidence: 79%

Effect of monoclonal antibody to pertussis toxin on toxin activity

Sato¹,

Sato²,

Ito³

et al. 1987

Infect Immun

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“…Previous studies have attributed the lymphocytosis to a toxin released by the organism that inhibits migration of lymphocytes into lymphoid tissue, resulting in an accumulation of small mature lymphocytes in the peripheral blood. 4,5 Some reports indicated that this lymphocytosis was due to increases in both T and B cells. 6,7 One report suggested that the lymphocytosis was due to a proportional increase in both CD4+ and CD8+ T cells, while other studies demonstrated a selective increase in CD4+ T cells.…”

Section: Discussionmentioning

confidence: 99%

A marked decrease in l‐selectin expression by leucocytes in infants with Bordetella pertussis infection: leucocytosis explained?

Hodge

Markus

et al. 2003

Respirology

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L-Selectin expression mediates extravasation of leucocytes into tissues and is important for homing of peripheral blood lymphocytes to lymph nodes. The significant down-regulation of L-selectin on leucocytes in pertussis infection may prevent leucocyte migration to areas of infection and homing and adhesion of T and B cells to peripheral lymphoid tissues. The increase in lymphocytes with Th1 phenotype may be required for effective immune response to the infective organism. These data provide a possible explanation for the absolute leucocytosis observed in this disease.

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“…It has long been believed that once PT is elaborated it becomes irreversibly bound to target tissues and therefore, once established, the disease would be unresponsive to therapy. Adler and Morse have shown that PT-induced lymphocytosis is reversible with anti-PT antibodies (3). In studies with the aerosol challenge model, a few investigators have suggested that PT antibodies could also reverse other manifestations of illness in mice, such as mortality and weight loss, even when given 3 days after established infection.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Pertussis Immunoglobulin (P-IGIV) in the Aerosol Challenge Model

Bruss

Siber

1999

Clin Diagn Lab Immunol

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Pertussis in infants is often severe, resulting in prolonged hospitalization. Treatment is limited to supportive care. Antibiotics do not significantly alter the course of the disease unless administered during the catarrhal phase. Therapies directed at pertussis toxin, a major virulence factor of Bordetella pertussis, may be beneficial. This study uses the aerosol challenge model to further examine the protective effects of P-IGIV, a new intravenous immunoglobulin product, which has high levels of pertussis toxin antibodies. P-IGIV was prepared as a 4% immunoglobulin G (IgG) solution from the pooled donor plasma from donors immunized with inactivated pertussis toxoid. The IgG pertussis toxin antibody concentration in P-IGIV is >7-fold higher than conventional intravenous immunoglobulin products. In the aerosol challenge model, P-IGIV-treated mice exhibited a dose-dependent decrease in mortality when monitored for 28 days postchallenge. P-IGIV in doses of 2,800, 1,400, and 350 mg/kg significantly reduced mortality compared to saline (P < 0.01)- and human IGIV (P < 0.01)-treated controls. The 50% protective dose of pertussis toxin antibodies in P-IGIV was 147 μg/ml. Recovery of weight gain and normalization of leukocyte counts occurred in all P-IGIV-treated groups but did not exhibit dose-dependent characteristics. Even after 7 days of infection, P-IGIV reversed the effects of pertussis in mice. This study provides further evidence that pertussis toxin antibodies not only play a role in passive protection but can also reverse symptoms of established disease in mice. We feel that P-IGIV deserves further evaluation in children hospitalized with severe pertussis.

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Interaction of Lymphoid and Nonlymphoid Cells with the Lymphocytosis-Promoting Factor of Bordetella pertussis

Cited by 12 publications

References 6 publications

Effect of monoclonal antibody to pertussis toxin on toxin activity

Effect of monoclonal antibody to pertussis toxin on toxin activity

A marked decrease in l‐selectin expression by leucocytes in infants with Bordetella pertussis infection: leucocytosis explained?

Protective Effects of Pertussis Immunoglobulin (P-IGIV) in the Aerosol Challenge Model

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