Interaction of matrix metalloproteinases with pulmonary pollutants: Table 1–

Dagouassat, Maylis; Lanone, Sophie; Boczkowski, Jorge

doi:10.1183/09031936.00195811

Cited by 40 publications

(25 citation statements)

References 94 publications

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“…A role for both ROS and metalloprotease activity has been reported for particle-and NP-induced cytokine gene expression [17,74,75]. Here enhanced gene expressions of HO-1, MMP-1 and MMP-9 were only seen at later time-points.…”

Section: Discussionmentioning

confidence: 66%

Pro-inflammatory effects of crystalline- and nano-sized non-crystalline silica particles in a 3D alveolar model

et al. 2020

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Background: Silica nanoparticles (SiNPs) are among the most widely manufactured and used nanoparticles. Concerns about potential health effects of SiNPs have therefore risen. Using a 3D tri-culture model of the alveolar lung barrier we examined effects of exposure to SiNPs (Si10) and crystalline silica (quartz; Min-U-Sil) in the apical compartment consisting of human alveolar epithelial A549 cells and THP-1-derived macrophages, as well as in the basolateral compartment with Ea.hy926 endothelial cells. Inflammation-related responses were measured by ELISA and gene expression. Results: Exposure to both Si10 and Min-U-Sil induced gene expression and release of CXCL8, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1α (IL-1α) and interleukin-1β (IL-1β) in a concentration-dependent manner. Cytokine/chemokine expression and protein levels were highest in the apical compartment. Si10 and Min-U-Sil also induced expression of adhesion molecules ICAM-1 and E-selectin in the apical compartment. In the basolateral endothelial compartment we observed marked, but postponed effects on expression of all these genes, but only at the highest particle concentrations. Geneexpressions of heme oxygenase-1 (HO-1) and the metalloproteases (MMP-1 and MMP-9) were less affected. The IL-1 receptor antagonist (IL-1RA), markedly reduced effects of Si10 and Min-U-Sil exposures on gene expression of cytokines and adhesion molecules, as well as cytokine-release in both compartments. Conclusions: Si10 and Min-U-Sil induced gene expression and release of pro-inflammatory cytokines/adhesion molecules at both the epithelial/macrophage and endothelial side of a 3D tri-culture. Responses in the basolateral endothelial cells were only induced at high concentrations, and seemed to be mediated by IL-1α/β released from the apical epithelial cells and macrophages.

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Section: Discussionmentioning

confidence: 66%

Pro-inflammatory effects of crystalline- and nano-sized non-crystalline silica particles in a 3D alveolar model

et al. 2020

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“…This PM-VOC lung damage occurs because VOCs, adsorbed on particle surfaces, promote more inflammation than PM alone [137,145]. Yet VOCs, the most dangerous part of PM, can constitute about 60 percent of airborne-PM mass [146]. Because outgassing VOCs make no-safe-dose PM more harmful, especially for still-developing lungs, this data gap should be closed.…”

Section: Airborne-exposure Data Gaps Especially For Particulate-metamentioning

confidence: 99%

Protecting Children from Toxic Waste: Data-Usability Evaluation Can Deter Flawed Cleanup

Shrader‐Frechette

Biondo

2020

IJERPH

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Nearly 25 percent of US children live within 2 km of toxic-waste sites, most of which are in urban areas. They face higher rates of cancer than adults, partly because the dominant contaminants at most US hazardous-waste sites include genotoxic carcinogens, like trichloroethylene, that are much more harmful to children. The purpose of this article is to help protect the public, especially children, from these threats and to improve toxics-remediation by beginning to test our hypothesis: If site-remediation assessments fail data-usability evaluation (DUE), they likely compromise later cleanups and public health, especially children’s health. To begin hypothesis-testing, we perform a focused DUE for an unremediated, Pasadena, California toxic site. Our DUE methods are (a) comparing project-specific, remediation-assessment data with the remediation-assessment conceptual site model (CSM), in order to identify data gaps, and (b) using data-gap directionality to assess possible determinate bias (whether reported toxics risks are lower/higher than true values). Our results reveal (1) major CSM data gaps, particularly regarding Pasadena-toxic-site risks to children; (2) determinate bias, namely, risk underestimation; thus (3) likely inadequate remediation. Our discussion shows that if these results are generalizable, requiring routine, independent, DUEs might deter flawed toxic-site assessment/cleanup and resulting health threats, especially to children.

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“…Air pollutants, consisting of complex and varying mixtures of different size and composition particles suspended in the air was called PM. Factories, power plants, refuse incinerators, automobile, construction activities, fires and natural windblown dust are some of the main sources of PM (Poschl, 2005;Lazaridis et al, 2008;Dagouassat et al, 2012). Their major components are metals, organic compounds, material of biologic origin, ions, reactive gases, and the particle carbon core (Kampa and Castanas, 2008).…”

Section: Particulate Matter (Pm)mentioning

confidence: 99%