Interaction of mycobacterial glycolipids with host cells

Polar liPids of RUANIA ALBIDIFLAVA, a novEl MEMBEr of thE suBordEr MICROCOCCINEAE Ruania albidiflava represents a novel species and new genus within the suborder Micrococcineae in Actinobacteria classis. The aim of the studies was to establish a polar lipid profile of Ruania and to compare it with other representatives of actinobacteria. Additionally, isolation, purification and chemical characteristics of major lipid compounds were elaborated. Major lipids were isolated using column adsorption chromatography and purified by TLC and high performance liquid chromatography. The polar lipids of Ruania were phosphatidylglycerol, diphosphatidylglycerol and two unknown lipids containing sugar and phosphorus. GLC-Ms and methylation analysis of sugar part of major glycolipids revealed the presence of glycerol, inositol; and terminal residues of mannose and galactosamine. Fatty acids were mainly iso and anteiso C15:0 branched. Comparative TLC showed its glycolipid profile of Ruania different from previously reported for Rothia, Arthrobacter, Micrococcus and Propionibacterium, but was similar to Oerskovia which belongs to Micrococcineae suborder.

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“…PIM are abundant in some actinobacteria f. ex. in Mycobacterium and are involved in the interactions of mycobacteria with host cells [11,1].…”

Section: Fіg 6 Maldi-tof Mass Spectra Of Glycolіpіd G1 and G2 Of Ruania Albidiflavamentioning

confidence: 99%

Polar Lipids of Ruania Albidiflava, a Novel Member of the Suborder Micrococcineae

Пащак

Каспров

Хуанг

et al. 2009

Microbiology&Biotechnology

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“…This is known as non-serovar-specific GPL (nsGPLs) and represents the main product of M. smegmatis GPLs. In M. avium GPLs present a more complicated structure in which an additional rhamnose and oligosaccharide residues are added to 6-deoxy- l- talose, resulting in serovar-specific GPLs [7, 9, 10]. These molecules, which may represent more than 70 % of total surface lipids [11], are required for cell aggregation, sliding motility and biofilm formation [12–15].…”

Section: Introductionmentioning

confidence: 99%

Inactivation of MSMEG_0412 gene drastically affects surface related properties of Mycobacterium smegmatis

et al. 2016

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BackgroundThe outermost layer of mycobacterial cell wall is rich in lipids and glycolipids, surface molecules which differ among species. Mycobacterium smegmatis, an attractive model for the study of both pathogenic and non-pathogenic mycobacteria, presents glycopeptidolipids (GPLs). All the genes necessary for the biosynthesis of such molecules are clustered in a single region of 65 kb and among them, the msmeg_0412 gene has not been characterized yet. Here we report the isolation and subsequent analysis of a MSMEG_0412 null mutant strain.ResultsThe inactivation of the msmeg_0412 gene had a drastic impact on bacterial surface properties which resulted in the lack of sliding motility, altered biofilm formation and enhanced drug susceptibility. The GPLs analysis showed that the observed mutant phenotype was due to GPLs deficiencies on the mycobacterial cell wall. In addition, we report that the expression of the gene is enhanced in the presence of lipidic substrates and that the encoded protein has a membrane localization.Conclusion msmeg_0412 plays a crucial role for GPLs production and translocation on M. smegmatis surface. Its deletion alters the surface properties and the antibiotic permeability of the mycobacterial cell barrier.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0888-z) contains supplementary material, which is available to authorized users.

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