Interaction of Mycobacterium tuberculosis with MH-S, an immortalized murine alveolar macrophage cell line: a comparision with primary murine macrophages

Melo, Maico DaPonte; Stokes, Richard W.

doi:10.1054/tuld.1999.0228

Cited by 16 publications

(16 citation statements)

References 28 publications

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“…While MH-S cells, as well as primary AMs, typically respond to both intracellular and extracellular bacterial pathogens [10 – 13]. However, Reading and colleagues [14] reported that the BJx109 strain of the human viral pathogen, Influenza A (H3N2) readily infected both MH-S cells and primary macrophages from mouse bronchoalveolar lavage, while the PR8 strain of Influenza A H1N1 did not.…”

Section: 0 Introductionmentioning

confidence: 99%

Immortalized MH-S cells lack defining features of primary alveolar macrophages and do not support mouse pneumovirus replication

et al. 2016

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The SV-40-transformed MH-S cell line maintains some, but not all, features of primary alveolar macrophages (AMs) from BALB/c mice. We show here that MH-S cells produce inflammatory cytokines IL-6 and CXCL10 in response to challenge with Gram-positive Lactobacillus reuteri, and to TLR2 and NOD2 ligands Pam3CSK4 and MDP, respectively. In contrast, although wild-type AMs are infected in vivo by pneumonia virus of mice (PVM), no virus replication was detected in MH-S cells. Interestingly, the surface immunophenotype of MH-S cells (CD11c+Siglec F−) differs from that of wild-type AMs (CD11c+ Siglec F+) and is similar to that of immature AMs isolated from granulocyte macrophage-colony stimulating factor (GM-CSF) gene-deleted mice; AMs from GM-CSF−/− mice also support PVM replication. However, MH-S cells do not express the GM-CSF receptor alpha chain (CD116) and do not respond to GM-CSF. Due to these unusual features, MH-S cells should be used with caution as experimental models of AMs.

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Section: 0 Introductionmentioning

confidence: 99%

Immortalized MH-S cells lack defining features of primary alveolar macrophages and do not support mouse pneumovirus replication

et al. 2016

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“…Recently, a murine alveolar macrophage cell line, MH-S, has been shown to be a useful model for intracellular bacterial pathogens that cause respiratory diseases, including Mycobacterium tuberculosis [8] and Chlamydia pneumophila [9]. MH-S cells retain functional, cytochemical and antigenic characteristics of untransformed alveolar macrophages [10] and can be easily maintained inde¢nitely as a homogeneous population.…”

Section: Introductionmentioning

confidence: 99%

Infection of murine macrophage cell lines by Legionella pneumophila

Yan

Cirillo

2004

FEMS Microbiology Letters

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Legionella pneumophila causes pneumonia by infecting alveolar macrophages. Although several model systems have been used for L. pneumophila virulence studies, no detailed comparisons have been made between them. An ideal in vitro virulence model should be cost-effective, easy to obtain in large amounts and as relevant as possible to the actual disease. We compared the MH-S cell line to human peripheral blood monocyte-derived macrophages and the J774A.1 cell line. We found that the interactions of L. pneumophila with MH-S at the cellular level resemble those of human primary monocyte-derived macrophages, suggesting that these cells provide a valuable model for this bacterial pathogen.

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“…Primary macrophages are natural (not immortalized) and are more representative of the actual in vivo situation, but they are usually harder to obtain and are more variable, especially macrophages from human donors. There are many mouse macrophage cell lines available, including the widely used J774 line and MH-S cells, the latter being an immortalized alveolar macrophage cell line whose behavior is very similar to that of primary mouse alveolar macrophages (191). Since the mouse is the most widely used animal model, there are advantages to using macrophages from this mammal, including reagent availability, as discussed above.…”

Section: Models For Measuring M Tuberculosis Virulencementioning

confidence: 99%

Mycobacterium tuberculosisPathogenesis and Molecular Determinants of Virulence

2003

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Tuberculosis (TB), one of the oldest known human diseases. is still is one of the major causes of mortality, since two million people die each year from this malady. TB has many manifestations, affecting bone, the central nervous system, and many other organ systems, but it is primarily a pulmonary disease that is initiated by the deposition of Mycobacterium tuberculosis, contained in aerosol droplets, onto lung alveolar surfaces. From this point, the progression of the disease can have several outcomes, determined largely by the response of the host immune system. The efficacy of this response is affected by intrinsic factors such as the genetics of the immune system as well as extrinsic factors, e.g., insults to the immune system and the nutritional and physiological state of the host. In addition, the pathogen may play a role in disease progression since some M. tuberculosis strains are reportedly more virulent than others, as defined by increased transmissibility as well as being associated with higher morbidity and mortality in infected individuals. Despite the widespread use of an attenuated live vaccine and several antibiotics, there is more TB than ever before, requiring new vaccines and drugs and more specific and rapid diagnostics. Researchers are utilizing information obtained from the complete sequence of the M. tuberculosis genome and from new genetic and physiological methods to identify targets in M. tuberculosis that will aid in the development of these sorely needed antitubercular agents

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Interaction of Mycobacterium tuberculosis with MH-S, an immortalized murine alveolar macrophage cell line: a comparision with primary murine macrophages

Cited by 16 publications

References 28 publications

Immortalized MH-S cells lack defining features of primary alveolar macrophages and do not support mouse pneumovirus replication

Immortalized MH-S cells lack defining features of primary alveolar macrophages and do not support mouse pneumovirus replication

Infection of murine macrophage cell lines by Legionella pneumophila

Mycobacterium tuberculosisPathogenesis and Molecular Determinants of Virulence

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