Interaction of mycoplasma virus type 2 with cellular components of Acholeplasma laidlawii strain JA1

Abstract

Mycoplasma virus type 2 was shown to adsorb specifically to intact cells, membranes, and lipoglycan of Acholeplasma laidlawii strain JA1 but not to these components of Acholeplasma oculi. The oligosaccharide chain of the lipoglycan defined the specificity of the receptor site since deacylation not only did not reduce adsorption but increased it threefold. Actual adsorption of virus to lipoglycan was demonstrated by sucrose density gradient separation of the virus-lipoglycan complex. A strain of A. laidlawii, JA1r, resistant to infection with mycoplasma virus type 2, was incapable of adsorbing the virus and was devoid of lipoglycan.

Adsorption of mycoplasmavirus MV-L2 to Acholeplasma laidlawii: effects of changes in the acyl-chain composition of membrane lipids

“…The kinetics of attachment are usually described as a pseudofirst-order reaction (3,19,33). Such kinetics of adsorption of MV-L2 to A. laidlawii were described previously (1,17,26). Liss and Heiland studied the adsorption of MV-L2 to three different strains of A. laidlawii and found that viruses produced from one strain differed in their ability to adsorb to the other strains: adsorption was always faster to the homologous strain (17).…”