Interaction of nanoparticles at the DEP microelectrode interface under high conductance conditions

Krishnan, Rajaram; Dehlinger, Dietrich; Gemmen, Gregory J.; Mifflin, Robert L.; Esener, Sadik C.; Heller, Michael

doi:10.1016/j.elecom.2009.06.033

Cited by 30 publications

(50 citation statements)

References 14 publications

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“…Until recently, the need to carry out DEP under low conductance conditions has limited practical applications of DEP based technologies [32][33][34]. The use of DEP under high conductance conditions has now been clearly demonstrated for separations of nanoparticles and high molecular weight (HMW) DNA [35] and cancer-related cell free circulating (cfc) DNA and virus [36] directly from un-diluted whole blood.…”

Section: Biophotonicsmentioning

confidence: 99%

“…The array comprises a 10 Â 10 grid of vapor-deposited 80 mm circular platinum electrodes at a centerto-center pitch of 200 mm [33,34]. The electrode array was powered by an Agilent 33250A function generator capable of operation up to 80 MHz at 20 V PP (7.07 V RMS with a sine wave) when using a high impedance load.…”

Section: Electrode Array and Power Sourcementioning

confidence: 99%

“…A deposited layer of silicon dioxide insulates the traces from the fluid but leaves the electrodes exposed. To protect analytes in solution from electrochemical effects at the surface, the array is coated with a layer of polyacrylamide hydrogel ( Figure 2) [33,34]. When the array is active, current flowing through the fluid induces localized heating above each electrode.…”

Section: Electrode Array and Power Sourcementioning

confidence: 99%

“…Concentration via DEP was performed using a 10 kHz sine wave at 20 V PP ; at this frequency, the nanoparticles experience pDEP and move towards the high field regions [33,34]. No wash step was required for imaging.…”

Section: Experimental Samplesmentioning

confidence: 99%

“…The separation of polystyrene nanoparticles in high conductance buffers and blood has been demonstrated through prior work [33][34][35][36], but cannot be reliably repeated in blood for lower levels. The nanoparticles used in this research are coated with carboxylate groups, which increase the surface potential and provide a net negative charge.…”

Section: Separation Of Dna From Whole Bloodmentioning

confidence: 99%

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Low level epifluorescent detection of nanoparticles and DNA on dielectrophoretic microarrays

McCanna¹,

Sonnenberg²,

Heller³

2013

Journal of Biophotonics

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Common epifluorescent microscopy lacks the sensitivity to detect low levels of analytes directly in clinical samples, such as drug delivery nanoparticles or disease related DNA biomarkers. Advanced systems such as confocal microscopes may improve detection, but several factors limit their applications. This study now demonstrates that combining an epifluorescent microscope with a dielectrophoretic (DEP) microelectrode array device enables the detection of nanoparticles and DNA biomarkers at clinically relevant levels. Using DEP microarray devices, nanoparticles and DNA biomarkers are rapidly isolated and concentrated onto specific microscopic locations where they are easily detected by epifluorescent microscopy. In this study, 40nm nanoparticles were detected down to 2–3 × 103/ul levels and DNA was detected down to the 200 pg/ml level. The synergy of epifluorescent microscopy and DEP microarray devices provides a new paradigm for DNA biomarker diagnostics and the monitoring of drug delivery nanoparticle concentrations. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Section: Biophotonicsmentioning

confidence: 99%

Section: Electrode Array and Power Sourcementioning

confidence: 99%

Section: Electrode Array and Power Sourcementioning

confidence: 99%

Section: Experimental Samplesmentioning

confidence: 99%

Section: Separation Of Dna From Whole Bloodmentioning

confidence: 99%

See 3 more Smart Citations

Low level epifluorescent detection of nanoparticles and DNA on dielectrophoretic microarrays

McCanna¹,

Sonnenberg²,

Heller³

2013

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

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Dielectrophoretic isolation of DNA and nanoparticles from blood

Sonnenberg

Marciniak²,

Krishnan³

et al. 2012

Electrophoresis

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The ability to effectively detect disease‐related DNA biomarkers and drug delivery nanoparticles directly in blood is a major challenge for viable diagnostics and therapy monitoring. A DEP method has been developed which allows the rapid isolation, concentration and detection of DNA and nanoparticles directly from human and rat whole blood. Using a microarray device operating at 20 V peak‐to‐peak and 10 kHz, a wide range of high molecular weight (HMW)‐DNA and nanoparticles were concentrated into high‐field regions by positive DEP, while the blood cells were concentrated into the low‐field regions by negative DEP. A simple fluidic wash removes the blood cells while the DNA and nanoparticles remain concentrated in the DEP high‐field regions where they can be detected by fluorescence. HMW‐DNA could be detected at 260 ng/mL, which is a detection level suitable for analysis of disease‐related cell‐free circulating DNA biomarkers. Fluorescent 40 nm nanoparticles could be detected at 9.5 × 109 particles/mL, which is a level suitable for monitoring drug delivery nanoparticles. The ability to rapidly isolate and detect DNA biomarkers and nanoparticles from undiluted whole blood will benefit many diagnostic applications by significantly reducing sample preparation time and complexity.

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Dielectrophoretic isolation and detection of cfc‐DNA nanoparticulate biomarkers and virus from blood

Sonnenberg

Marciniak

McCanna³

et al. 2013

Electrophoresis

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Dielectrophoretic (DEP) microarray devices allow important cellular nanoparticulate biomarkers and virus to be rapidly isolated, concentrated and detected directly from clinical and biological samples. A variety of sub-micron nanoparticulate entities including cell free circulating (cfc) DNA, mitochondria and virus can be isolated into DEP high-field areas on microelectrodes, while blood cells and other micron-size entities become isolated into DEP low-field areas between the microelectrodes. The nanoparticulate entities are held in the DEP high-field areas while cells are washed away along with proteins and other small molecules which are not affected by the DEP electric fields. DEP carried out on 20 µL of whole blood obtained from Chronic Lymphocytic Leukemia (CLL) patients showed a considerable amount of SYBR Green stained DNA fluorescent material concentrated in the DEP high-field regions. Whole blood obtained from healthy individuals showed little or no fluorescent DNA materials in the DEP high-field regions. Fluorescent T7 bacteriophage virus could be isolated directly from blood samples, and fluorescently stained mitochondria could be isolated from biological buffer samples. Using newer DEP microarray devices, high molecular weight (hmw) DNA could be isolated from serum and detected at levels as low as 8–16 ng/mL.

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Interaction of nanoparticles at the DEP microelectrode interface under high conductance conditions

Cited by 30 publications

References 14 publications

Low level epifluorescent detection of nanoparticles and DNA on dielectrophoretic microarrays

Low level epifluorescent detection of nanoparticles and DNA on dielectrophoretic microarrays

Dielectrophoretic isolation of DNA and nanoparticles from blood

Dielectrophoretic isolation and detection of cfc‐DNA nanoparticulate biomarkers and virus from blood

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