Interaction of Nanoparticles with Blood Components and Associated Pathophysiological Effects

Cruz, G. González de la; Rodríguez-Fragoso, P.; JorgeReyes-Esparza,; Rodríguez‐López, Anahí; Gómez-Cansino, Rocío; Rodríguez‐Fragoso, Lourdes

doi:10.5772/intechopen.69386

Cited by 34 publications

(17 citation statements)

References 121 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, studying nanomaterial behaviour in these types of cells is crucial since they will interact with NPs once introduced into the blood torrent. 25 We investigated NT B 700 behaviour in PBMC, based on results previously obtained on tumoral U937 cell line. Firstly, we incubated PBMCs with NT B 700 (64 µg/mL, as best beforehand condition) for different times, from 1 h to 48 h. We observed that fluorescence (=internalized NPs) increased in a timedependent manner, both for lymphocytes and monocytes (gating strategy based on morphological features on FSC vs. SSC dot plot), as for U937 cells ( Figure 6A), even if for lymphocytes was not so evident because of the lower fluorescence signal.…”

Section: Investigation Of Nt B 700 Nanoparticle Uptake Mechanism and mentioning

confidence: 99%

See 1 more Smart Citation

Uptake and Intracellular Trafficking Studies of Multiple Dye-Doped Core-Shell Silica Nanoparticles in Lymphoid and Myeloid Cells

Sola

Canonico

Montanari

et al. 2021

NSA

View full text Add to dashboard Cite

Introduction Since most biologically active macromolecules are natural nanostructures, operating in the same scale of biomolecules gives the great advantage to enhance the interaction with cellular components. Noteworthy efforts in nanotechnology, particularly in biomedical and pharmaceutical fields, have propelled a high number of studies on the biological effects of nanomaterials. Moreover, the determination of specific physicochemical properties of nanomaterials is crucial for the evaluation and design of novel safe and efficient therapeutics and diagnostic tools. In this in vitro study, we report a physicochemical characterisation of fluorescent silica nanoparticles (NPs), interacting with biological models (U937 and PBMC cells), describing the specific triggered biologic response. Methods Flow Cytometric and Confocal analyses are the main method platforms. However TEM, NTA, DLS, and chemical procedures to synthesize NPs were employed. Results NT B 700 NPs, employed in this study, are fluorescent core-shell silica nanoparticles, synthesized through a micelle-assisted method, where the fluorescence energy transfer process, known as FRET, occurs at a high efficiency rate. Using flow cytometry and confocal microscopy, we observed that NT B 700 NP uptake seemed to be a rapid, concentration-, energy- and cell type-dependent process, which did not induce significant cytotoxic effects. We did not observe a preferred route of internalization, although their size and the possible aggregated state could influence their extrusion. At this level of analysis, our investigation focuses on lysosome and mitochondria pathways, highlighting that both are involved in NP co-localization. Despite the main mitochondria localization, NPs did not induce a significant increase of intracellular ROS, known inductors of apoptosis, during the time course of analyses. Finally, both lymphoid and myeloid cells are able to release NPs, essential to their biosafety. Discussion These data allow to consider NT B 700 NPs a promising platform for future development of a multifunctional system, by combining imaging and localized therapeutic applications in a unique tool.

show abstract

Section: Investigation Of Nt B 700 Nanoparticle Uptake Mechanism and mentioning

confidence: 99%

“…Studying nanomaterial behaviour in these types of cells is fundamental since they will interact with NPs once introduced into the blood torrent. 25…”

Section: Introductionmentioning

confidence: 99%

Uptake and Intracellular Trafficking Studies of Multiple Dye-Doped Core-Shell Silica Nanoparticles in Lymphoid and Myeloid Cells

Sola

Canonico

Montanari

et al. 2021

NSA

View full text Add to dashboard Cite

show abstract

“…No morphological alterations occurred in RBCs and WBCs as a result of their exposure to NPs. The RBCs were negatively charged, which prevented cells from coming into contact with them [ 18 ]. Thus, we could assume that the balance between the attractive and repulsive electrostatic forces between RBCs was not disturbed by the amphiphilic dendrons.…”

Section: Discussionmentioning

confidence: 99%

“…The former condition is linked to platelet clumping—as a result, reported platelet counts were lower than their actual counts in the blood, because automated counters cannot differentiate platelet clumps from individual cells. The latter could be linked to a deficiency of the plasma factors prothrombin, fibrinogen, factor V, or factor X [ 16 , 18 ]. Assuming that the reference range of the APTT was 30–40 s and the reference range of the PTT was 60–70 s [ 19 ], the observed out-of-range prolongation in APTT-SS was linked to the increasing number of accessible positively charged groups in the D2C2 experimental condition.…”

Section: Discussionmentioning

confidence: 99%

Blood Compatibility of Amphiphilic Phosphorous Dendrons—Prospective Drug Nanocarriers

et al. 2021

View full text Add to dashboard Cite

Dendrons are branched synthetic polymers suitable for preparation of nanosized drug delivery systems. Their interactions with biological systems are mainly predetermined by their chemical structure, terminal groups, surface charge, and the number of branched layers (generation). Any new compound intended to be used, alone or in combination, for medical purposes in humans must be compatible with blood. This study combined results from in vitro experiments on human blood and from laboratory experiments designed to assess the effect of amphiphilic phosphorous dendrons on blood components and model membranes, and to examine the presence and nature of interactions leading to a potential safety concern. The changes in hematological and coagulation parameters upon the addition of dendrons in the concentration range of 2–10 µM were monitored. We found that only the combination of higher concentration and higher generation of the dendron affected the selected clinically relevant parameters: it significantly decreased platelet count and plateletcrit, shortened thrombin time, and increased activated partial thromboplastin time. At the same time, occasional small-sized platelet clumps in blood films under the light microscope were observed. We further investigated aggregation propensity of the positively charged dendrons in model conditions using zwitterionic and negatively charged liposomes. The observed changes in size and zeta potential indicated the electrostatic nature of the interaction. Overall, we proved that the low-generation amphiphilic phosphorous dendrons were compatible with blood within the studied concentration range. However, interactions between high-generation dendrons at bulk concentrations above 10 µM and platelets and/or clotting factors cannot be excluded.

show abstract

“…Also, with the blood being the first point of contact within the body, many pathophysiological events can be elicited due to the interaction of the NPs with the different blood components. This could result in the activation of the immune system, coagulation or fibrinolysis cascades, hemolysis and protein adsorption [220]. As previously commented, the PEGylation of the delivery agents can increase the circulation time after IV and also help to avoid the immune system and improve biocompatibility [221], but peripheral accumulation and non-selective delivery may still be verified.…”

Section: 'Conventional' Administration Routesmentioning

confidence: 99%

Breaking Barriers: Bioinspired Strategies for Targeted Neuronal Delivery to the Central Nervous System

et al. 2020

View full text Add to dashboard Cite

Central nervous system (CNS) disorders encompass a vast spectrum of pathological conditions and represent a growing concern worldwide. Despite the high social and clinical interest in trying to solve these pathologies, there are many challenges to bridge in order to achieve an effective therapy. One of the main obstacles to advancements in this field that has hampered many of the therapeutic strategies proposed to date is the presence of the CNS barriers that restrict the access to the brain. However, adequate brain biodistribution and neuronal cells specific accumulation in the targeted site also represent major hurdles to the attainment of a successful CNS treatment. Over the last few years, nanotechnology has taken a step forward towards the development of therapeutics in neurologic diseases and different approaches have been developed to surpass these obstacles. The versatility of the designed nanocarriers in terms of physical and chemical properties, and the possibility to functionalize them with specific moieties, have resulted in improved neurotargeted delivery profiles. With the concomitant progress in biology research, many of these strategies have been inspired by nature and have taken advantage of physiological processes to achieve brain delivery. Here, the different nanosystems and targeting moieties used to achieve a neuronal delivery reported in the open literature are comprehensively reviewed and critically discussed, with emphasis on the most recent bioinspired advances in the field. Finally, we express our view on the paramount challenges in targeted neuronal delivery that need to be overcome for these promising therapeutics to move from the bench to the bedside.

show abstract

Interaction of Nanoparticles with Blood Components and Associated Pathophysiological Effects

Cited by 34 publications

References 121 publications

Uptake and Intracellular Trafficking Studies of Multiple Dye-Doped Core-Shell Silica Nanoparticles in Lymphoid and Myeloid Cells

Uptake and Intracellular Trafficking Studies of Multiple Dye-Doped Core-Shell Silica Nanoparticles in Lymphoid and Myeloid Cells

Blood Compatibility of Amphiphilic Phosphorous Dendrons—Prospective Drug Nanocarriers

Breaking Barriers: Bioinspired Strategies for Targeted Neuronal Delivery to the Central Nervous System

Contact Info

Product

Resources

About