Interaction of Nanoparticles with Lipid Membrane

Roiter, Yuri; Ornatska, Maryna; Rammohan, Aravind; Balakrishnan, Jitendra; Heine, David R.; Minko, Sergiy

doi:10.1021/nl080080l

Cited by 339 publications

(378 citation statements)

References 30 publications

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“…[43][44][45][46][47] The occurrence of the interaction depends on whether the amount of energy released from the binding of the MSNs with the RBC membrane (E i ) is able to overcome the amount of free energy required to bend the membrane and adapt to the surface of MSNs (E b ). The former energy is associated with the external surface area (i.e., accessible silanols) of MSN, 30 while the latter is proportional to the curvature or inversely proportional to the square of the radius (r) of the particle.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, since surface curvature decreases with particle size, the bending energy required to wrap the large particles (E b ) is lower than the one needed to wrap the smaller particles. 43 This combination makes membrane wrapping and engulfment of l-MSN thermodynamically favorable. On the contrary, in order for the RBC membranes to wrap around smaller s-MSNs, they would have to attain a larger curvature (steeper angles over smaller areas) than they need for wrapping around the larger particles.…”

Section: Resultsmentioning

confidence: 99%

“…Similar explanations on the effect of particle size on membrane wrapping have been reported elsewhere. 43,44 Hence, the interaction of MSNs with RBC membranes and the hemolytic activity depend on not only particle size but also their external surface area, as well.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Zhao¹,

Sun²,

Zhang³

et al. 2011

ACS Nano

516

440

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The interactions of mesoporous silica nanoparticles (MSNs) of different particle sizes and surface properties with human red blood cell (RBC) membranes were investigated by membrane filtration, flow cytometry, and various microscopic techniques. Small MCM-41-type MSNs (∼100 nm) were found to adsorb to the surface of RBCs without disturbing the membrane or morphology. In contrast, adsorption of large SBA-15-type MSNs (∼600 nm) to RBCs induced a strong local membrane deformation leading to spiculation of RBCs, internalization of the particles, and eventual hemolysis. In addition, the relationship between the degree of MSN surface functionalization and the degree of its interaction with RBC, as well as the effect of RBC−MSN interaction on cellular deformability, were investigated. The results presented here provide a better understanding of the mechanisms of RBC−MSN interaction and the hemolytic activity of MSNs and will assist in the rational design of hemocompatible MSNs for intravenous drug delivery and in vivo imaging. R ecent advancements in particle size and morphology control of mesoporous materials have led to the creation of nano-and submicrometer-sized mesoporous silica nanoparticles (MSNs). [1][2][3][4][5] The MSN materials with well-ordered cylindrical pore structures, such as MCM-41 and SBA-15, have attracted special interest in the biomedical field. 1 The large surface areas and pore volumes of these materials allow the efficient adsorption of a wide range of molecules, including small drugs, 6-10 therapeutic proteins, 11-13 antibiotics, 14,15 and antibodies. 16 Therefore, these materials have been proposed for use as potential vehicles for biomedical imaging, real-time diagnosis, and controlled delivery of multiple therapeutic agents. [6][7][8]10,[17][18][19][20][21][22][23][24][25] Despite the considerable interest in the biomedical applications of MSNs, relatively few studies have been published on the biocompatibility of the two most common types of MSNs (MCM-41 and SBA-15). [26][27][28][29] Asefa and co-workers reported that the cellular bioenergetics (cellular respiration and ATP levels) were inhibited remarkably by large SBA-15 nanoparticles, but the inhibition was greatly reduced by smaller MCM-41-type nanoparticles. 26 These differences in the disruption of cellular bioenergetics are believed to be caused by the different surface areas, number of surface silanol groups, and/or particle sizes of both types of material. A recent study by Kohane and collaborators on the systemic effects of MCM-41 (particle size ∼150 nm) and SBA-15 (particle size ∼800 nm) MSNs in live animals revealed interesting findings regarding their biocompatibility. 27 While large doses of mesoporous silicas administered subcutaneously to mice appear to be relatively harmless, the same doses given intravenously or intraperitoneally were lethal. 27 A possible reason for the severe systemic toxicity of MSNs when injected intravenously could be the interactions of the nanoparticles with blood cells.Our initial studies...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Zhao¹,

Sun²,

Zhang³

et al. 2011

ACS Nano

516

440

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“…16,17 An atomic force microscopy study revealed that substrate-supported silica nanoparticles with diameters ranging from 1.2 to 22 nm pierced the L-a-dimyristoyl phosphatidylcholine bilayer, whereas particles outside of that size range supported the L-adimyristoyl phosphatidylcholine membrane without pore formation ( Figure 1a). The incomplete bilayer formation was attributed to the unfavorable bending energy of the membrane forming a highly curved structure.…”

Section: Formation Of Lipid-nanostructure Hybrids and Their Structurementioning

confidence: 99%

Lipid-nanostructure hybrids and their applications in nanobiotechnology

Lee

Nam

2013

NPG Asia Mater

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Cell membranes contain a variety of lipids and functional proteins that offer active platforms that organize the membrane components into functional assemblies and perform biologically important reactions. The dynamic and complex nature of the membranes makes them attractive materials, but at the same time, researchers report substantial experimental uncertainty in controlling the membrane reactions and extracting valuable information. The fascinating new structures and properties of nanomaterials could be utilized in addressing aforementioned issues, and the design and synthesis of lipid-nanostructure hybrids could be beneficial to the research areas in lipid-membrane biotechnology and nanobiotechnology. These hybrid structures possess dimensions that are comparable to that of biological molecules and structures and physicochemical properties that arise from both lipids and nanomaterials. Therefore, lipid-nanostructure hybrids offer additional options for control of the synthesized structures, provide new insight in understanding nanostructures and biological systems and allow the mimicking of functional subcellular membrane components and monitoring of the membrane-associated reactions in a highly sensitive and controllable manner. In this review, we present recent advances in the synthesis of various lipid-nanostructure hybrids and the application of these structures in biotechnology and nanotechnology. We further describe the scientific and practical applications of lipid-nanostructure hybrids for detecting membrane-targeting molecules, interfacing nanostructures with live cells and creating membrane-mimicking platforms to investigate various intercellular processes.

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“…These norms have been repetitively validated in experimental studies, 18,19 and larger nanoparticles (i.e., >22 nm) in turn are known to be wrapped up by a lipid bilayer. 12,15 These boundaries can be cumbersome when designing application-oriented nanocontainers, as specific nanoparticle properties (i.e., optical, thermal and/or magnetic) ; which make them desirable in the first place ; are mostly governed by their size. Superparamagnetic iron oxide nanoparticles (SPIONs) are a general paradigm: their superparamagnetic features make them ideal thermoregulators 24 for various medically oriented tasks (i.e., hyperthermia 25,26 or drug release 17,18,27,28 ) and are presently valued as contrast agents in magnetic resonance imaging.…”

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confidence: 99%

Insertion of Nanoparticle Clusters into Vesicle Bilayers

et al. 2014

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. z These authors contributed equally to this work.C ombining nanoparticles with liposomes is a highly elaborative methodology in the emerging fields of nanomedicine and nanobiotechnology. Not only can the well-known benefits 1 of liposomes be enhanced for specific applications, but their established scope of functionality (e.g., targeted drug delivery 2À7 ) can also be widely expanded to fulfill additional tasks and thereby act as eclectic toolboxes at a nanoscale. 8 Lipid bilayer membranes alone are significantly relevant materials for a broad range of academic disciplines. Their interactions with nanoparticles have been gradually studied over the past years, particularly in the context of nanoparticle transport across cellular membranes. 9À12 Notable experimental and theoretical studies have addressed different variables (e.g., the nanoparticle size or lipid composition) and their effects on adhesion and wrapping or nanoparticle-induced pore formation. 12À15 These concepts have been complemented by, for example, molecular dynamics simulations 16 or mesoscale thermodynamic modeling 13 and provide, among others, more accurate descriptions of phospholipid membranes to determine the role and effects of nanoparticles on lipid bilayers in general.In the context of stimuli-responsive liposomeÀnanoparticle hybrids for drug delivery, embedding hydrophobic nanoparticles directly within the liposomal lipid bilayer has become a popular approach. There are several standards in the literature on this subject, 17À21 which also present operative methodologies to accomplish this task. In this wide-ranging field, leading studies have focused on using very small nanoparticles * Address correspondence to alke.fink@unifr.ch. ABSTRACTA major contemporary concern in developing effective liposomeÀnanoparticle hybrids is the present inclusion size limitation of nanoparticles betw vesicle bilayers, which is considered to be around 6.5 nm in diameter. In this article, we present experimental observations backed by theore considerations which show that greater structures can be incorporated within vesicle membranes by promoting the clustering of nanoparticles be liposome formation. Cryo-transmission electron microscopy and cryo-electron tomography confirm these observations at unprecedented detail underpin that the liposome membranes can accommodate flexible structures of up to 60 nm in size. These results imply that this material is more vers in terms of inclusion capabilities and consequently widens the opportunities in developing multivalent vesicles for nanobiotechnology applications

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Interaction of Nanoparticles with Lipid Membrane

Cited by 339 publications

References 30 publications

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Lipid-nanostructure hybrids and their applications in nanobiotechnology

Insertion of Nanoparticle Clusters into Vesicle Bilayers

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