Interaction of nickel with mutagens in the induction of sister chromatid exchanges in human lymphocytes

Nickel subsulfide is similar to potassium dichromate in protecting normal human fibroblasts from the mutagenic effects of benzo[a]pyrene diolepoxide

“…These workers reported that soluble nickel sulfate produced an enhanced or synergistic effect with several DNA-damaging agents in various rodent cell lines [Christie, 1989]. The same laboratory recently reported an antagonistic effect of soluble nickel sulfate with some of the same DNA-damaging agents in human lymphocytes [Katsifis et al, 1996]. These data show that soluble nickel sulfate can also produce an antagonistic effect and support the hypothesis that the difference between our results and the previous studies may be due to a species difference.…”

“…More recent data, however, suggests that a simple additive or synergistic response to multiple carcinogens may not be the total answer. Katsifis et al [1996] showed that nickel treatment can actually have an antagonistic effect and protect human lymphocytes from the induction of sister chromatid exchanges due to UV-light, X-rays, and chromium. Work from our laboratory has shown that chromium can protect normal human fibroblasts from the mutagenic effects of BPDE [Tesfai et al, 1998].…”

Genotoxicity modulation by cadmium treatment: Studies in the Drosophila wing spot test

“…To test for possible interactions between CC and PDC or EMS, we calculated an interaction factor (IF) following the method of Schlesinger et al [1992] and using an adaptation of the formula applied by Katsifis et al [1996]. The significance of IF differences was evaluated with Dunnett's test using SPSS v. 11.5 software (SPSS, Chicago, IL).…”

Genotoxicity of fixation devices analyzed by the frequencies of sister chromatid exchange

“…In comparison with the controls consisting of healthy, smoker and non-smoker subjects, Jelmert et al [24] found a statistically significant increase in the frequency of SCE in the peripheral lymphocytes of the 42 workers directly exposed to nickel and chromium ions in a welding factory manufacturing metal arches containing stainless steel. In an in-vitro study, Katsifis et al [28] incubated the cell cultures containing NiSO 4 and chromium with the heparinized blood samples from non-smokers who were not previously exposed to the dusts of heavy metals and not showing signs of DNA damage. They indicated that nickel (II) and chromium (VI) increased the frequency of SCE in peripheral lymphocytes.…”