Interaction of perfluorooctanoic acid with extracellular polymeric substances - Role of protein

Yan, Wangwang; Qian, Tingting; Zhang, Liang; Wang, Li; Zhou, Yan

doi:10.1016/j.jhazmat.2020.123381

Cited by 66 publications

(19 citation statements)

References 37 publications

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“…It can be seen that with the increase of TBBPA levels, the UV–visible absorption spectra of EPS at 210 nm increased gradually. Generally, the absorbance of UV at 210 nm is related to protein framework conformation, indicating that TBBPA was mainly bound by proteins within EPS . The EEM fluorescence analysis was used to further explore the mechanism and the binding processes between TBBPA and EPS (Figure h–j).…”

Section: Resultsmentioning

confidence: 92%

“…Generally, the absorbance of UV at 210 nm is related to protein framework conformation, indicating that TBBPA was mainly bound by proteins within EPS. 41 The EEM fluorescence analysis was used to further explore the mechanism and the binding processes between TBBPA and EPS (Figure 3h−j). It can be observed that the fluorescence intensity of Peak A (Ex/Em of 220−225/295− 310 nm), which respectively corresponded to the spectra of tyrosine-like proteins in EPS, was gradually enhanced with the TBBPA concentration increasing from 0 to 2.0 mg/L.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Hormesis-Like Effects of Tetrabromobisphenol A on Anaerobic Digestion: Responses of Metabolic Activity and Microbial Community

Liu

et al. 2022

Environ. Sci. Technol.

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Tetrabromobisphenol A (TBBPA) has extensive applications in various fields; its release into ecosystems and the potential toxic effects on organisms are becoming major concerns. Here, we investigated the effects of TBBPA on anaerobic digestion, whose process is closely related to the carbon cycles under anaerobic conditions. The results revealed that TBBPA exhibited dose-dependent hormesis-like effects on methane production from glucose, i.e., the presence of 0.1 mg/L TBBPA increased the methane production rate by 8.79%, but 1.0−4.0 mg/L TBBPA caused 3.45−28.98% of decrement. We found that TBBPA was bound by the tyrosine-like proteins of the extracellular polymeric substances of anaerobes and induced the increase of reactive oxygen species, whose slight accumulation stimulated the metabolism activities but high accumulation increased the apoptosis of anaerobes. Owing to the differences between individual anaerobes in tolerance, TBBPA at 0.1 mg/L stimulated the acidogenesis and hydrogenotrophic methanogenesis, whereas higher levels (i.e., 1.0−4.0 mg/L) severely restrained all of the processes of acidogenesis, acetogenesis, and methanogenesis. Along with the accumulation of bisphenol A (BPA) produced from TBBPA by Longilinea sp. and Pseudomonas sp., the methanogenic pathway was partly shifted from acetate-dependent to hydrogen-dependent direction, and the activities of carbon monoxide dehydrogenase and acetyl-CoA decarbonylase/synthase were inhibited, while acetate kinase and F420 were hormetically affected. These findings elucidated the mechanism of anaerobic syntrophic consortium responses to TBBPA, supplementing the potential environmental risks of brominated flame retardants.

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Section: Resultsmentioning

confidence: 92%

Section: ■ Results and Discussionmentioning

confidence: 99%

Hormesis-Like Effects of Tetrabromobisphenol A on Anaerobic Digestion: Responses of Metabolic Activity and Microbial Community

Liu

et al. 2022

Environ. Sci. Technol.

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“…Figure a-b displayed the UV–vis of EPSs without and with a 10 or 20 mg/L BmimCl addition. It can be seen that the UV–vis spectra of EPSs with the BmimCl addition (i.e., EPSs-BmimCl complex) was lower than that of the sum of the sole EPS and BmimCl around 215 nm (1.66 vs 1.44 and 3.04 vs 2.83), suggesting that the entered BmimCl was bound with anaerobic EPSs . The adsorption around 215 nm reflects the framework conformation of protein, indirectly indicating that this binding was associated with proteins .…”

Section: Resultsmentioning

confidence: 99%

1-Butyl-3-methylimidazolium Chloride Affects Anaerobic Digestion through Altering Organics Transformation, Cell Viability, and Microbial Community

Liu

et al. 2023

Environ. Sci. Technol.

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1-Butyl-3-methylimidazolium chloride (BmimCl), an imidazolium-based ionic liquid, is considered the representative emerging persistent aquatic pollutant, and its environmental toxicity has attracted a growing concern. However, most of the investigations focused on monocultures or a single organism, with little information available on the complex syntrophic consortium that dominates the complex and successional biochemical processes, such as anaerobic digestion. In this study, the effect of BmimCl at environmentally relevant levels on glucose anaerobic digestion was therefore investigated in several laboratory-scale mesophilic anaerobic digesters to provide such support. Experimental results showed that BmimCl at 1–20 mg/L inhibited the methane production rate by 3.50–31.03%, and 20 mg/L BmimCl inhibited butyrate, hydrogen, and acetate biotransformation by 14.29%, 36.36%, and 11.57%, respectively. Toxicological mechanism studies revealed that extracellular polymeric substances (EPSs) adsorbed and accumulated BmimCl through carboxyl, amino, and hydroxyl groups, which destroyed the EPSs’ conformational structure, thereby leading to the inactivation of microbial cells. MiSeq sequencing data indicated that the abundance of Clostridium_sensu_stricto_1, Bacteroides, and Methanothrix decreased by 6.01%, 7.02%, and 18.45%, respectively, in response to 20 mg/L BmimCl. Molecular ecological network analysis showed that compared with the control, the lower network complexity, fewer keystone taxa, and fewer associations among microbial taxa were found in the BmimCl-present digester, indicating the reduced stability of the microbial community.

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“…, Ca 2+ and Mg 2+ ) may also serve as ion bridges to mediate electrostatic sorption. 41–43 It is worth noting that the hydrophobic interaction between biofilms and organic pollutants may play a more important role than electrostatic repulsion in water. 44–46 Polysaccharides in biofilms may be the main sorption sites for some compounds, e.g.…”

Section: Resultsmentioning

confidence: 99%

“…, tryptophan and tyrosine) in proteins of EPS to promote hydrophobic interaction. 43 But the contribution of this potential sorption process to the observed reduction in R s and mass accumulations in pre-fouled o-DGT samplers was uncertain.…”

Section: Resultsmentioning

confidence: 99%

Effects of biofouling on the uptake of perfluorinated alkyl acids by organic-diffusive gradients in thin films passive samplers

Challis

et al. 2022

Environ. Sci.: Processes Impacts

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Interaction of perfluorooctanoic acid with extracellular polymeric substances - Role of protein

Cited by 66 publications

References 37 publications

Hormesis-Like Effects of Tetrabromobisphenol A on Anaerobic Digestion: Responses of Metabolic Activity and Microbial Community

Hormesis-Like Effects of Tetrabromobisphenol A on Anaerobic Digestion: Responses of Metabolic Activity and Microbial Community

1-Butyl-3-methylimidazolium Chloride Affects Anaerobic Digestion through Altering Organics Transformation, Cell Viability, and Microbial Community

Effects of biofouling on the uptake of perfluorinated alkyl acids by organic-diffusive gradients in thin films passive samplers

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