Interaction of Phototoxic Compounds With Cells in Tissue Culture

Freeman, Robert G.

doi:10.1001/archderm.1970.04000110037006

Cited by 17 publications

(5 citation statements)

References 30 publications

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“…To determine whether or not membrane damage could play a role in the photosensitivity caused by these compounds, their sensitizing effects in the red blood cell (RBC) photohemolysis system was investigated. C P Z has been reported to photosensitize RBC lysis (Freeman, 1970;Kahn and Fleischaker, 1971), and, interestingly preirradiated C P Z was also able to lyse RBC (Johnson, 1974). The mechanism of photosensitivity caused by PTL has not been studied previously.…”

Section: Introductionmentioning

confidence: 99%

Chlorpromazine and Protriptyline Phototoxicity: Photosensitized, Oxygen Independent Red Cell Hemolysis

Kochevar

Lamola²

1979

Photochem & Photobiology

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Abstract—Red blood cell lysis photosensitized by chlorpromazine and protriptyline was investigated. Oxygen independent sensitized photohemolysis was observed with both compounds. When chlorpromazine and protriptyline were irradiated in the absence of red cells, both formed photoproducts which lysed red cells. These results indicate that chlorpromazine and protriptyline represent a new mechanistic class of phototoxic compounds which photosensitize membrane disruption by oxygen independent processes.

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Section: Introductionmentioning

confidence: 99%

Chlorpromazine and Protriptyline Phototoxicity: Photosensitized, Oxygen Independent Red Cell Hemolysis

Kochevar

Lamola²

1979

Photochem & Photobiology

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“…It has been shown that some photosensitizers may induce de layed photohemolysis. not visible until al least 24 hours post irradiation (4,8) probably represent ing some minor membrane alterations. but the mechanisms are poorly understood.…”

Section: Discussionmentioning

confidence: 99%

Membrane damage caused by 8-MOP and UVA-treatment of cultivated cells

Wennersten¹

1979

Acta Derm Venereol

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Treatment of red blood cells with a combination of 8-MOP and UVA did not cause any significant hemolysis under the experimental conditions used, and the deuterium test for identification of singlet oxygen mediated reactions was negative. However, similar experiments performed on in vitro cultivated human glia cells caused conspicuous surface membrane alterations, as revealed by scanning electron microscopy. The alterations were seen at higher concentration levels than was expected and might be secondary to earlier appearing intracellular events seen at lower concentrations. The clinical significance of membrane alterations for the results obtained with PUVA therapy require further investigation.

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“…The persistence of 8-methoxypsoralen in the irradiated area may be due to the preferred binding of this compound to DNA (Freeman, 1970), without any detectable change in the colour of fluorescence.…”

Section: Discussionmentioning

confidence: 99%

Effects of long wave ultraviolet radiation on photosensitizing and related compounds. I

Alani

2006

British Journal of Dermatology

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The effects of long ultraviolet radiation (UVA) on the fluorescence of twenty-four photosensitizing and related compounds were studied. Each compound was applied in two areas on the skin of hairless mice. One area was irradiated. The fluorescence in both areas was then inspected every day until it disappeared.Persistence and change of colour of the fluorescence as a direct immediate effect of UVA radiation on compounds having photoallergic potential, i.e. salicylanilide, halogenated salicylanilide and phenothiazine derivatives, was observed. Compounds known for their phototoxic effects, i.e. acridine dyes, did not show such changes after irradiation, while 8-methoxypsoralen showed persistence of fluorescence without change of colour.The fluorescence of most photosensitizing compounds has been known for many years, but very little is known concerning the effects of ultraviolet radiation on the fluorescence of these compounds, especially when applied on the skin. Therefore, it was considered of interest to compare the fluorescence of such compounds. applied on the skin of hairless mice, before and after long wave ultraviolet (UVA) irradiation. Twenty-four fluorescent compounds were included in this study, nineteen of which were either potential photoallergens or structurally related thereto, the remaining flve being phototoxic compounds (Hjorth & Fregert, 1968; Pathak, 1969). MATERIALS AND METHODS Test compoundsA 1% solution (10 mg/ml) of each compound in dimethyl sulphoxide (DMSO) was made. The compounds included salicylanilide, twelve halogenated salicylanilides (HS), six phenothiazine derivatives, four acridine dyes and 8-methoxypsoralen. These were obtained in reagent form from various suppliers (Table i).

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Interaction of Phototoxic Compounds With Cells in Tissue Culture

Cited by 17 publications

References 30 publications

Chlorpromazine and Protriptyline Phototoxicity: Photosensitized, Oxygen Independent Red Cell Hemolysis

Chlorpromazine and Protriptyline Phototoxicity: Photosensitized, Oxygen Independent Red Cell Hemolysis

Membrane damage caused by 8-MOP and UVA-treatment of cultivated cells

Effects of long wave ultraviolet radiation on photosensitizing and related compounds. I

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