Interaction of Plant Growth Regulators in Regeneration Processes

Schraudolf, H.; Reinert, J.

doi:10.1038/184465a0

Cited by 66 publications

(16 citation statements)

References 2 publications

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“…However, explants cultured on a comparatively higher concentration of BA (3.0-4.0 mg L −1 ) produced 1.6-2.0 shoots only and no simultaneous rooting was observed. This was in accordance with the observation made by Schraudolf and Reinert (1959) that, high concentrations of cytokinin generally inhibit or delay root formation. In MS supplemented with 1.0-2.0 mg L −1 BA, only fewer shoots (1.3 and 1.6) and roots (1.6 and 2.0) were recorded (Table 2; Fig.…”

Section: Resultssupporting

confidence: 77%

Micropropagation of Hedychium coronarium J. Koenig through rhizome bud

Mohanty

Behera

Swain

et al. 2013

Physiol Mol Biol Plants

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An optimized protocol was developed for in vitro plant regeneration of a medicinally important herb Hedychium coronarium J. Koenig using sprouted buds of rhizomes. The rhizomes with sprouted bud were inoculated on Murashige and Skoog (Physiol Plant 15:473-497, 1962) was most effective. In this medium, best shoots (3.6) and roots (4.0) regeneration was observed simultaneously with an average shoot and root length of 4.7 cm and 4.2 cm respectively. Regeneration of shoots and roots in the same medium at the same time (One step shoot and root regeneration) reduced the time for production of in vitro plantlets and eliminates the media cost of rooting. Cent-percent (100 %) success in plant establishment was observed in both gradual acclimatization process as well as when plants were directly transferred to outdoor in clay pots containing a mixture of garden soil and sand (2:1) without any sequential acclimatization stage.

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Section: Resultssupporting

confidence: 77%

Micropropagation of Hedychium coronarium J. Koenig through rhizome bud

Mohanty

Behera

Swain

et al. 2013

Physiol Mol Biol Plants

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“…In general cytokinins inhibit root induction in stem cuttings (Schrandolf and Reinert 1959), however, root initiation was observed in pea cuttings when applied at very low concentrations (Eriksen 1974). In this investigation, BAP, a natural cytokinin (Nandi et al 1989), either alone or in combination with IBA or NAA significantly improved rooting of runner segments.…”

Section: Discussionmentioning

confidence: 68%

Propagation and Conservation of Picrorhiza kurrooa Royle ex Benth.: An Endangered Himalayan Medicinal Herb of High Commercial Value

2006

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Picrorhiza kurrooa Royle ex Benth., a high value medicinal herb of alpine Himalaya and a source of hepatoprotective picrosides, is listed as 'endangered' due to heavy collection from its natural habitat. The present report deals with successful propagation of this species using both conventional and in vitro techniques. Vegetative propagation was achieved by rooting runner cuttings with indole-3-butyric acid (IBA) or a-naphtheleneacetic acid (NAA) treatment before planting. Nearly 87% rooting success was achieved by treatment of cuttings with 50.0 lM IBA. Seeds were given a presoaking treatment with gibberellic acid (GA 3 ), 6-benzylaminopurine (BAP) or a combination of both to influence germination. More than 11-fold improvement in germination was recorded in seeds treated with 250.0 lM GA 3 . In vitro shoot multiplication was achieved through sprouting of axillary buds using nodal segment. Multiple shoots were formed following culture for 3 weeks on Murashige and Skoog (MS; 1962. Physiologia Plantarum 15: 473-497) medium containing 1.0 lM BAP. Cent percent rooting success, without basal callus formation, was observed when individual microshoots were placed in MS medium supplemented with IBA. The plantlets raised using conventional as well as tissue culture methods were hardened and successfully established in the experimental field located at 2450 m elevation. In addition, strategies have been discussed to encourage cultivation and in situ conservation of this highly valued medicinal herb so as to reduce pressure on its natural populations.

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“…This basipetal gradient of regeneration also occurs in leaf segments of begonia and Saintpaulia ionantha and has been linked to differences in auxin levels in the leaf (Schraudolf and Reinert 1959;Kukułczanka and Suszyńska 1972). However, the endogenous levels of active IAA did not proof to be different in basal and apical lily explants.…”

Section: Apetala2/ethylene Responsive Factor (Ap2/erf) Transcription mentioning

confidence: 92%

Mechanisms of vegetative propagation in bulbs : a molecular approach

Moreno-Pachon

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Interaction of Plant Growth Regulators in Regeneration Processes

Cited by 66 publications

References 2 publications

Micropropagation of Hedychium coronarium J. Koenig through rhizome bud

Micropropagation of Hedychium coronarium J. Koenig through rhizome bud

Propagation and Conservation of Picrorhiza kurrooa Royle ex Benth.: An Endangered Himalayan Medicinal Herb of High Commercial Value

Mechanisms of vegetative propagation in bulbs : a molecular approach

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