Interaction of PLD1b with actin in antigen-stimulated mast cells

Farquhar, Michelle J.; Powner, Dale; Levine, Barry A.; Wright, Michelle H.; Ladds, Graham; Hodgkin, Matthew N.

doi:10.1016/j.cellsig.2006.07.016

Cited by 20 publications

(23 citation statements)

References 28 publications

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“…The remaining caspase 3 site in the N-terminus of PLD1b at EEVD 41 Y, is known to be the region of PKC interaction and phosphorylation sites ( Figure 1B) [17]. Two highscoring putative caspase-3 sites were also identified in the N-terminus of human PLD2a ( Figure 1A) at DELD 16 S and DEVD 28 T. Of the 3 caspase sites identified in human PLD1b, DFID 669 R is conserved in the rat, but not the mouse, PLD1 sequence.…”

Section: Resultsmentioning

confidence: 99%

“…To determine if PLD1b was a caspase-3 substrate, purified recombinant and catalytically active [6,17] GST-PLD1b (1g) was incubated with purified Figure 2b shows quantification of radioactivity within the 148kDa parent GFPhPLD1b (wild-type and mutants) band following incubation with caspase-3 from 3 separate experiments. As a control human GFP-hPLD2a (134kDa) was also expressed using in vitro coupled transcription and translation.…”

Section: Resultsmentioning

confidence: 99%

“…Radioactivity per band was plotted as a percentage of total radioactivity per lane (units). Recombinant PLD1b was produced as described [6,17], digested with caspase 3 (as above) and fragments identified by western blotting using the specified antibodies. …”

Section: Generation Of Vectors Expressing Gfp-pld Mutatantsmentioning

confidence: 99%

“…We have recently shown a functional link between role and regulation of PLD1b through association with Factin during antigen-induced secretion in RBL-2H3 cells [17].…”

Section: Introductionmentioning

confidence: 99%

“…PLD-catalysed PtdCho hydrolysis plays a regulatory role in important processes such as the oxidative burst in neutrophils [8,9], secretion [10][11][12], mitogenesis [13,14] and cellular shape change and adhesion [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

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Identification of caspase 3 motifs and critical aspartate residues in human phospholipase D1b and phospholipase D2a

Wright

Farquhar

Aletrari

et al. 2008

Biochemical and Biophysical Research Communications

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Stimulation of mammalian cells frequently initiates phospholipase D-catalysedhydrolysis of phosphatidylcholine in the plasma membrane to yield phosphatidic acid (PA) a novel lipid messenger. PA plays a regulatory role in important cellular processes such as secretion, cellular shape change and movement. A number of studies have highlighted that PLD-based signalling also plays a pro-mitogenic and pro-survival role in cells and therefore anti-apoptotic. We show that human PLD1b and PLD2a contain functional caspase-3 cleavage sites and identify the critical aspartate residues within PLD1b that affect its activation by phorbol esters and attenuate phosphatidylcholine hydrolysis during apoptosis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Generation Of Vectors Expressing Gfp-pld Mutatantsmentioning

confidence: 99%

“…We have recently shown a functional link between role and regulation of PLD1b through association with Factin during antigen-induced secretion in RBL-2H3 cells [17].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identification of caspase 3 motifs and critical aspartate residues in human phospholipase D1b and phospholipase D2a

Wright

Farquhar

Aletrari

et al. 2008

Biochemical and Biophysical Research Communications

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Survey of the year 2007 commercial optical biosensor literature

Rich

Myszka

2008

J of Molecular Recognition

166

121

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In 2007, 1179 papers were published that involved the application of optical biosensors. Reported developments in instrument hardware, assay design, and immobilization chemistry continue to improve the technology's throughput, sensitivity, and utility. Compared to recent years, the widest range of platforms, both traditional format and array-based, were used. However, as in the past, we found a disappointingly low percentage of well-executed experiments and thoughtful data interpretation. We are alarmed by the high frequency of suboptimal data and over-interpreted results in the literature. Fortunately, learning to visually recognize good--and more importantly, bad--data is easy. Using examples from the literature, we outline several features of biosensor responses that indicate experimental artifacts versus actual binding events. Our goal is to have everyone, from benchtop scientists to project managers and manuscript reviewers, become astute judges of biosensor results using nothing more than their eyes.

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Phosphoinositides in Chemotaxis

Weiger

Parent

2012

Subcellular Biochemistry

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Interaction of PLD1b with actin in antigen-stimulated mast cells

Cited by 20 publications

References 28 publications

Identification of caspase 3 motifs and critical aspartate residues in human phospholipase D1b and phospholipase D2a

Identification of caspase 3 motifs and critical aspartate residues in human phospholipase D1b and phospholipase D2a

Survey of the year 2007 commercial optical biosensor literature

Phosphoinositides in Chemotaxis

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