Interaction of poly (ethylene oxide)–poly (propylene oxide)–poly (ethylene oxide) triblock copolymer of molecular weight 2800 with sodium dodecylsulfate (SDS) micelles: some physicochemical studies

James, Jilly; Vellaichami, S.; Krishnan, R.; Samikannu, S.; Mandal, Asit Baran

doi:10.1016/j.chemphys.2004.10.022

Cited by 38 publications

(55 citation statements)

References 45 publications

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“…This is the proportion predicted by the experiments on PC bilayers described later to determine the partition coefficients of PA and LPPC. The surface tension decreases with concentration more slowly than in neat LPPC solutions, as expected for mixtures of surfactants and hydrophobic additives ( 22 ). The data show that micelles begin to form when the LPPC concentration is 1.01 M/L.…”

Section: Resultssupporting

confidence: 53%

Quantitation of lysolipids, fatty acids, and phospholipase A2 activity and correlation with membrane polarity

Singh

Ranganathan

2012

Journal of Lipid Research

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is a measure of the concentration of FA present ( 2, 3 ). This property, useful for detection of low levels of FAs, led to the development of a sensitive fl uorescence assay for phospholipase A 2 (PLA 2 ) activity ( 4 ).ADIFAB also binds lysophospholipids, anionic and some zwitterionic detergents. This fact, although intrinsically useful for quantitating not just FAs but lysophosphatidylcholines (LPCs) as well, calls into question the earlier PLA 2 assay that took into account only FA binding. The goals of this work were to establish the quantitative and linear nature of ADIFAB fl uorescence response to FA and LPC, develop quantitative assays for detecting both FA and LPC in aqueous solutions as well as for continuous real time monitoring of PLA 2 hydrolysis at membrane interfaces expressing activity in absolute units of M of product/minute/mg of enzyme, and extend the applicability of the ADIFAB PLA 2 assay to more lipids and lipid bilayers.Phospholipase catalyzed hydrolysis of phospholipids at membrane interfaces produces FAs and lysophospholipids ( 5 ). The rate of hydrolysis depends on membrane physicochemical properties like membrane curvature, lipid composition, lipid chain length, and headgroup charge ( 6, 7 ). It is typically quite low at membrane interfaces and can be well below the precision levels of the standard pH-Stat method ( 8 ). The importance of PLA 2 activity to several physiological functions and membrane biochemistry demands sensitive methods not only to detect membrane hydrolysis but also to quantitatively and continuously monitor PLA 2 activity in real time in absolute units ( 9, 10 ). In this context, the fl uorescence response of the probe ADIFAB to the presence of hydrolysis products offers just such a method ( 1 ).The ADIFAB fl uorescence assay, fi rst reported by Richieri and Kleinfeld, relies on the shift in fl uorescence Acrylodan-labeled rat-intestinal fatty acid binding protein (ADIFAB) exhibits a fl uorescence emission peak at 432 nm when excited by light of wavelength 386 nm ( 1 ). The emission peak shifts to 505 nm upon binding an FA. The ADIFAB fl uorescence response depends on the type of FA. The ratio of the intensities at 505 nm to that at 432 nm

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Section: Resultssupporting

confidence: 53%

Quantitation of lysolipids, fatty acids, and phospholipase A2 activity and correlation with membrane polarity

Singh

Ranganathan

2012

Journal of Lipid Research

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“…If N agg is known for any system, it is possible to calculate K A and n. For detail analysis and calculation we referred to earlier publications [36,40]. Following Turro and Yekta [37]:…”

Section: The Cmc and Counter Ion Association Of Ctab In Aqueous And Imentioning

confidence: 99%

“…It is then apt to set up the following mass-action principle for the mixed/complexing system, assuming the following model: (1) In the above model, n number of molecules of ionic CTAB with a single substrate (drug) molecule form a mixed micelle (complex) with equilibrium association constant K A . We can write the following equation [36,40]:…”

Section: The Cmc and Counter Ion Association Of Ctab In Aqueous And Imentioning

confidence: 99%

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Thermodynamics studies on tyrosine-hydantoin drug–cetyltrimethylammonium bromide mixed micellar system

Tiwary

Mandal

Alam

et al. 2011

Colloids and Surfaces B: Biointerfaces

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“…The cmc values for the nonionic surfactants were determined from the intersection of two straight lines that were drawn using the least-squares method, as shown in Fig. 3 and presented in which is similar to the cmc values obtained using other methods 30,31 .…”

Section: Critical Micelle Concentrationmentioning

confidence: 92%

Interactions between Dyes and Surfactants in Inkjet Ink Used for Textiles

2011

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Interaction of poly (ethylene oxide)–poly (propylene oxide)–poly (ethylene oxide) triblock copolymer of molecular weight 2800 with sodium dodecylsulfate (SDS) micelles: some physicochemical studies

Cited by 38 publications

References 45 publications

Quantitation of lysolipids, fatty acids, and phospholipase A2 activity and correlation with membrane polarity

Quantitation of lysolipids, fatty acids, and phospholipase A2 activity and correlation with membrane polarity

Thermodynamics studies on tyrosine-hydantoin drug–cetyltrimethylammonium bromide mixed micellar system

Interactions between Dyes and Surfactants in Inkjet Ink Used for Textiles

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