Interaction of polypyridyl ruthenium (II) complex containing asymmetric ligand with DNA

Liu, Yun-Jun; Chao, Hui; Tan, Lifeng; Yuan, Yali; Wei, Wei; Ji, Liang‐Nian

doi:10.1016/j.jinorgbio.2004.10.030

Cited by 97 publications

(34 citation statements)

References 45 publications

(39 reference statements)

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“…These results suggested a cytotoxic effect of these molecules initially by inhibiting DNA synthesis, which will induce a decrease of protein synthesis resulting in diminished cell survival. This pattern corresponds to the effects described for some platinum or other ruthenium derivatives known to bind to DNA [27][28][29][30][31][32]. Photodynamic studies revealed that the nature of the pyridylporphyrin isomer (3-pyridyl or 4-pyridyl) was more important than the degree of substitution of the tetrapyrrole ring.…”

Section: Discussionsupporting

confidence: 77%

Combined arene ruthenium porphyrins as chemotherapeutics and photosensitizers for cancer therapy

et al. 2008

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Mononuclear 5-(4-pyridyl)-10,15,20-triphenylporphyrin and 5-(3-pyridyl)-10,15,20-triphenylporphyrin as well as tetranuclear 5,10,15,20-tetra(4-pyridyl)porphyrin (tetra-4-pp) and 5,10,15,20-tetra(3-pyridyl)porphyrin) (tetra-3-pp) arene ruthenium(II) derivatives (arene is C 6 H 5 Me or p-Pr i C 6 H 4 Me) were prepared and evaluated as potential dual photosensitizers and chemotherapeutics in human Me300 melanoma cells. In the absence of light, all tetranuclear complexes were cytotoxic (IC 50 B 20 lM), while the mononuclear derivatives were not (IC 50 C 100 lM). Kinetic studies of tritiated thymidine and tritiated leucine incorporations in cells exposed to a low concentration (5 lM) of tetranuclear p-cymene derivatives demonstrated a rapid inhibition of DNA synthesis, while protein synthesis was inhibited only later, suggesting arene ruthenium-DNA interactions as the initial cytotoxic process. All complexes exhibited phototoxicities toward melanoma cells when exposed to laser light of 652 nm. At low concentration (5 lM), LD 50 of the mononuclear derivatives was between 5 and 10 J/cm 2 , while for the tetranuclear derivatives LD 50 was approximately 2.5 J/cm 2 for the [Ru 4 (g 6 -arene) 4 (tetra-4-pp)Cl 8 ] complexes and less than 0.5 J/cm 2 for the [Ru 4 (g 6 -arene) 4 (tetra-3-pp)Cl 8 ] complexes. Examination of cells under a fluorescence microscope revealed the [Ru 4 (g 6 -arene) 4 (tetra-4-pp)Cl 8 ] complexes as cytoplasmic aggregates, whereas the [Ru 4 (g 6 -arene) 4 (tetra-3-pp)Cl 8 ] complexes were homogenously dispersed in the cytoplasm. Thus, these complexes present a dual synergistic effect with good properties of both the arene ruthenium chemotherapeutics and the porphyrin photosensitizer.

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Section: Discussionsupporting

confidence: 77%

Combined arene ruthenium porphyrins as chemotherapeutics and photosensitizers for cancer therapy

et al. 2008

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“…The amount of the supercoiled form of the plasmid DNA diminished gradually, whereas the open-circular form increased, with an increasing irradiation time. Similar results have been observed by others [26]. Under comparable experimental conditions, control experiments (untreated pBIND irradiated at 365 nm and pBIND in the dark) did not show observable cleavage (Fig.…”

Section: Resultssupporting

confidence: 91%

DNA-binding properties of ruthenium(II) complexes with the bidentate ligand 5-chloro-2-(phenylazo)pyridine

Ratanaphan

Nhukeaw

Temboot

et al. 2012

Transition Met Chem

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A bidentate ligand, 5-chloro-2-(phenylazo)pyridine (Clazpy), and its two polypyridyl ruthenium(II) complexes, [Ru(Clazpy) 2 bpy]Cl 2 Á7H 2 O (1) and [Ru(Clazpy) 2 phen]Cl 2 Á8H 2 O (2), were synthesized and characterized. The DNA-binding properties of these complexes with DNA, the breast cancer susceptibility gene 1 (BRCA1), and the pBIND plasmid DNA were probed by photocleavage, electronic absorption titration, ethidium bromide quenching, and thermal denaturation. Both complexes were found to bind to the BRCA1 fragment through the intercalative mode into the base pairs of DNA, and the DNA-binding constants (K b ) for 1 and 2 were 7.0 9 10 4 M -1 and 5.1 9 10 5 M -1 , respectively. In addition, both complexes enhanced the single-stranded cleavage of the plasmid DNA. Under comparable experimental conditions, 2 cleaved DNA more effectively than 1, in a dose-response manner. The data indicated that the binding affinity of these two complexes to DNA was dependent on the aromatic planarity and hydrophobicity of the intercalative polypyridyl ligand.

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“…6. for ruthenium bipyridine complexes containing imidazole derivatives [38,39]. In comparison to RuNaph, a reasonable explanation for the better interaction between Ru-Anth and DNA could be due to the higher molecular surface area available of the anthracene moiety for pi-stacking interactions.…”

Section: Dna Titration Monitored By Luminescencementioning

confidence: 98%

Ruthenium(II) bipyridine complexes with pendant anthracenyl and naphthyl moieties: A strategy for a ROS generator with DNA binding selectivity

Abreu

Diógenes

Lopes

et al. 2016

Inorganica Chimica Acta

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Interaction of polypyridyl ruthenium (II) complex containing asymmetric ligand with DNA

Cited by 97 publications

References 45 publications

Combined arene ruthenium porphyrins as chemotherapeutics and photosensitizers for cancer therapy

Combined arene ruthenium porphyrins as chemotherapeutics and photosensitizers for cancer therapy

DNA-binding properties of ruthenium(II) complexes with the bidentate ligand 5-chloro-2-(phenylazo)pyridine

Ruthenium(II) bipyridine complexes with pendant anthracenyl and naphthyl moieties: A strategy for a ROS generator with DNA binding selectivity

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