Interaction of porcine gastric mucin with various polycations and its influence on the boundary lubrication properties

Abstract

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Self-Assembled Mucin-Containing Microcarriers via Hard Templating on CaCO3 Crystals

“…The negative charge of mucin at neutral and alkaline pH is due to the presence of sialic acids (pKa = 2.6) located on the ends of the chains of a polysaccharide backbone [ 11 ]. Mucins from various sources have been employed to assemble multilayers using the following polycations: polyallylamine hydrochloride, poly-L-lysine, polyethylenimine, methylcellulose, chitosan, and lactoperoxidases (Mw 78 kDa, pI 8.3) [ 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. The thickness of the formed multilayers depends on pH and ionic strength.…”

“…This work aims at the development of new approaches for BAC microencapsulation based on the vaterite CaCO 3 crystals and mucin. Here the mucin from the porcine stomach has been used for the naturally-derived mucin widely employed for research [ 11 , 25 ]. The porcine gastric mucin has been shown to be structurally related to human gastric mucin [ 32 ] and is, therefore, a decent substitute of human gastric mucin because of its high availability and reduced number of ethical issues required for the research.…”

Progress and Current Trends in the Synthesis of Novel Polymers with Enhanced Mucoadhesive Properties

“…Both PAH and PDMAEMA are synthesized via radical polymerization techniques (e.g., vinyl addition, free radical, or controlled radical polymerization), employing the commercially available allyl amine and 2‐(dimethylamino)ethyl methacrylate monomers, respectively. Akin to chitosan, these synthetic materials were found to exhibit enhanced mucoadhesive properties in acidic environments owing to protonation of the amine derivatives . Furthermore these materials can be easily formulated into nanogels, liposomes, and films; however, in the case of PAH, toxicity issues have restricted its application somewhat .…”

Gastric mucus and mucuslike hydrogels: Thin film lubricating properties at soft interfaces

“…ratio 50:9 of PGM:PAA as in "biosimilar" mucus, 7 except that BSA and lipids were excluded), and they were denoted as PGM:b-PEI and PGM:PAA, respectively. The mixing ratios for the two PGM:polyelectrolyte mixtures were determined based on the optimization in previous studies for PGM:b-PEI 21,22 and PGM:PAA, 7 respectively. Neat (unmixed) PGM and b-PEI (100 mg/mL) solutions were also employed for comparison, and denoted as PGM or b-PEI, respectively.…”

“…[17][18][19][20] In addition, recent studies on the interaction between PGM and b-PEI have also shown associative interaction mainly via electrostatic attraction and improved lubricating properties in the dilute concentration regime (≤ 1 mg/mL in total concentration). 21,22 An illustration of the chemical structure of PAA and b-PEI, as well as the interaction mechanism with PGM, is presented in It is hypothesized that the associative interaction between PGM and the two polyelectrolytes may be extended to the high concentration regime (ca. 50 -100 mg/mL in concentration).…”