Interaction of positively-charged liposomes with blood: implications for their application in vivo

Senior, Judith; Trimble, Kevin R.; Maskiewicz, Richard

doi:10.1016/0005-2736(91)90160-a

Cited by 153 publications

(66 citation statements)

References 15 publications

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“…Serum-induced inhibition of lipoplex-mediated transfection has been often reported. [25][26][27][28] However, the transfection ability of the SucPG-complex did not decrease even at a serum concentration of 40%. In contrast, the transfection activity of the plain lipoplex decreased slightly as serum concentration of the medium increased up to 20%, although its serum sensitivity was not significant.…”

Section: Figure 5 Luciferase Activity Of Hela Cells Treated With Tranmentioning

confidence: 91%

Novel gene delivery systems: complexes of fusigenic polymer-modified liposomes and lipoplexes

et al. 2001

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We have previously developed the succinylated poly(glycidol)-modified liposome which becomes fusigenic under weakly acidic condition. In this report, we describe that complexation of this pH-sensitive, fusigenic liposome with a lipoplex consisting of 3␤-(N-(NЈ,NЈ-dimethylaminoethane) carbamoyl)cholesterol, dioleoylphosphatidylethanolamine and plasmid DNA gives efficient gene delivery systems. In this study, we prepared the complexes, which are termed SucPG-complexes, with a positively or negatively charged surface by mixing the lipoplex with varying amounts of the SucPG-modified liposomes. The positively charged SucPGcomplexes either bearing or not bearing a cell-specific

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Section: Figure 5 Luciferase Activity Of Hela Cells Treated With Tranmentioning

confidence: 91%

Novel gene delivery systems: complexes of fusigenic polymer-modified liposomes and lipoplexes

et al. 2001

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“…[108,109] As a less toxic alternative to SA, the cationic compounds dimethyldioctadecylammonium bromide (DDAB) or 3β-(N(N 1 N 1 -dimethylaminoethane) carbamoyl) cholesterol (DC-chol) can also target several skin and oral bacteria via electrostatic interactions. [76,[78][79][80] DC-chol containing liposomes showed the most effective targeting towards S. epidermidis while DDAB was the least effective targeting agent.…”

Section: Non-specific Targetingmentioning

confidence: 99%

Lipid and polymer nanoparticles for drug delivery to bacterial biofilms

Forier

Raemdonck

Smedt

et al. 2014

Journal of Controlled Release

372

320

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Biofilms are matrix-enclosed communities of bacteria that show increased antibiotic resistance and the capability to evade the immune system. They can cause recalcitrant infections which cannot be cured with classical antibiotic therapy. Drug delivery by lipid or polymer nanoparticles is considered a promising strategy for overcoming biofilm resistance. These particles are able to improve the delivery of antibiotics to the bacterial cells, thereby increasing the efficacy of the treatment. In this review we give an overview of the types of polymer and lipid nanoparticles that have been developed for this purpose. The antimicrobial activity of nanoparticle encapsulated antibiotics compared to the activity of the free antibiotic is discussed in detail. In addition, targeting and triggered drug release strategies to further improve the antimicrobial activity are reviewed. Finally, ample attention is given to advanced microscopy methods that shed light on the behavior of nanoparticles inside biofilms, allowing further optimization of the nanoformulations. Lipid and polymer nanoparticles were found to increase the antimicrobial efficacy in many cases. Strategies such as the use of fusogenic liposomes, targeting of the nanoparticles and triggered release of the antimicrobial agent ensured the delivery of the antimicrobial agent in close proximity of the bacterial cells, maximizing the exposure of the biofilm to the antimicrobial agent. The majority of the discussed papers still present data on the in vitro anti-biofilm activity of nanoformulations, indicating that there is an urgent need for more in vivo studies in this field.

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“…[15][16][17][18] Furthermore, intravenously injected cationic DNA complexes also encounter unintended cell types such as macrophages, monocytes, neutrophils, platelets, and erythrocytes, which are important potential mediators of toxicity. 9,19,20 Toxic manifestations of systemically administered cationic DNA complexes can range from red blood cell agglutination 21 to potent inflammatory reaction and elevated serum levels of liver enzymes. 9,22 Several studies have attempted to avoid such adverse interactions by including polyethyleneglycol (PEG) or proteins such as albumin or transferrin in the DNA complexes.…”

Section: Introductionmentioning

confidence: 99%

Recharging cationic DNA complexes with highly charged polyanions for in vitro and in vivo gene delivery

et al. 2003

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The intravenous delivery of plasmid DNA complexed with either cationic lipids (CL) or polyethyleneimine (PEI) enables high levels of foreign gene expression in lung. However, these cationic DNA complexes cause substantial toxicity. The present study found that the inclusion of polyacrylic acid (pAA) with DNA/polycation and DNA/CL complexes prevented the serum inhibition of the transfection complexes in cultured cells. The mechanism mediating this increase seems to involve both particle size enlargement due to flocculation and electrostatic shielding from opsonizing serum proteins. The use of pAA also increased the levels of lung expression in mice in vivo substantially above the levels achieved with just binary complexes of DNA and linear PEI (lPEI) or CL and reduced their toxicity. Also, the use of a 'chaser' injection of pAA 30 min after injection of the ternary DNA/lPEI/pAA complexes further aided this effort to reduce toxicity while not affecting foreign gene expression. By optimizing the amount of pAA, lPEI, and DNA within the ternary complexes and using the 'chaser' injection, substantial levels of lung expression were obtained while avoiding adverse effects in lung or liver. These developments will aid the use of cationic DNA complexes in animals and for eventual human gene therapy.

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Interaction of positively-charged liposomes with blood: implications for their application in vivo

Cited by 153 publications

References 15 publications

Novel gene delivery systems: complexes of fusigenic polymer-modified liposomes and lipoplexes

Novel gene delivery systems: complexes of fusigenic polymer-modified liposomes and lipoplexes

Lipid and polymer nanoparticles for drug delivery to bacterial biofilms

Recharging cationic DNA complexes with highly charged polyanions for in vitro and in vivo gene delivery

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