Interaction of proline, sugars, and anthocyanins during photosynthetic acclimation of Arabidopsis thaliana to drought stress

Sperdouli, Ilektra; Moustakas, Michael

doi:10.1016/j.jplph.2011.12.015

Cited by 284 publications

(198 citation statements)

References 70 publications

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“…Under stress conditions such as heavy metals, drought, temperature, etc., plants could be protected by a mechanism that increases its antioxidative properties, such as producing flavonoids, including anthocyanin, and antioxidative enzymes to overcome metal toxicity (Hung et al, 2008;Keilig and Ludwig-Müller, 2009;Sperdouli and Moustakas, 2012). Anthocyanins have been implied to confer tolerance to diverse environmental stressors (Close and Beadle, 2003;Gould, 2004).…”

Section: Discussionmentioning

confidence: 99%

Proline partially overcomes excess molybdenum toxicity in cabbage seedlings grown in vitro

Jutamas¹,

Huang²,

Lee³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. In vitro grown cabbage (Brassica oleracea var. capitata) seedlings exposed to excess molybdenum (Mo) ions exhibited severely reduced plant growth at the cotyledonary stage. Adding 80 mM proline (Pro) to the Mo-treated medium could help 50% seedlings to overcome the toxicity and grow true leaves. Under excess Mo stress, seedlings accumulated blue/purple anthocyanin in their cotyledons and hypocotyls. The anthocyanin content under Mo with 40 mM Pro was 4-fold higher than the control medium, MS with 40 mM Pro. The presence of Pro in the excess-Mo condition reduced chlorophyll a, whereas the chlorophyll b content was much higher than the control media of MS with and without Pro. Moreover, supplementing various concentrations of Pro into the Mo-stressed condition promoted the seedlings with higher antioxidant enzyme activities of superoxide dismutase, ascorbate peroxidate, and catalase. In addition, genes in the anthocyanin biosynthesis and accumulation pathways, phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), flavonone 3-hydroxylase (F3H), leucoanthocyanidin dioxygenase (LDOX), and glutathione-S-transferase (GST), were all upregulated. Our study indicated that, under excess Mo stress, the antioxidant activity of cabbage seedlings was induced in an attempt to protect plants from the Mo-induced toxicity and exacerbated growth. Pro, on the other hand, functioned in producing higher antioxidant enzyme activity to partially help recover plant growth.

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Section: Discussionmentioning

confidence: 99%

Proline partially overcomes excess molybdenum toxicity in cabbage seedlings grown in vitro

Jutamas¹,

Huang²,

Lee³

et al. 2013

Genet. Mol. Res.

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“…However, Hughes et al (2013) suggested that the role of anthocyanin compounds was likely not in osmotic protection because of low anthocyanin concentrations and their high metabolic cost compared to other solutes, such as proline and soluble sugars, which are typically found to be more effective in osmotic adjustment. A recent study by Sperdouli and Moustakas (2014) in A. thaliana suggested that anthocyanins can have an important antioxidant role under drought stress as drought-stressed leaves maintained oxidative compounds (such as malondialdehyde) within the same range as found in control leaves, thereby implying that a biochemical mechanism was in operation to cope with oxidative damage. Therefore, we can suggest, based on the above-mentioned reports, that higher expression of F3 0 H, F3 0 5 0 H, and UFGT genes under drought stress will allow the accumulation of tri-hydroxylated anthocyanin forms, giving a greater antioxidant capacity.…”

Section: Anthocyanin Accumulation Under Drought Stressmentioning

confidence: 92%

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

González‐Villagra

Kurepin

2017

Planta

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Main conclusion ABA is involved in anthocyanin synthesis through the regulation of microRNA156, augmenting the level of expression of anthocyanin synthesisrelated genes and, therefore, increasing anthocyanin level.Drought stress is the main cause of agricultural crop loss in the world. However, plants have developed mechanisms that allow them to tolerate drought stress conditions. At cellular level, drought stress induces changes in metabolite accumulation, including increases in anthocyanin levels due to upregulation of the anthocyanin biosynthetic pathway. Recent studies suggest that the higher anthocyanin content observed under drought stress conditions could be a consequence of a rise in the abscisic acid (ABA) concentration. This plant hormone crosses the plasma membrane by specific transporters, and it is recognized at the cytosolic level by receptors known as pyrabactin resistance (PYR)/regulatory component of ABA receptors (PYR/RCARs) that regulate downstream components. In this review, we discuss the hypothesis regarding the involvement of ABA in the regulation of microRNA156 (miRNA156), which is upregulated as part of dehydration stress responsiveness in different species. The miRNA156 upregulation produces a greater level of anthocyanin gene expression, forming the multienzyme complex that will synthesize an increased level of anthocyanins at the cytosolic face of the rough endoplasmic reticulum (RER). After synthesis, anthocyanins are transported from the RER to the vacuole by two possible models of transport: (1) membrane vesicle-mediated transport, or (2) membrane transporter-mediated transport. Thus, the aim was to analyze the recent findings on synthesis, transport and the possible mechanism by which ABA could increase anthocyanin synthesis under drought stress conditions potentially throughout microRNA156 (miRNA156).

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“…There was a significant increase in abscisic acid (ABA) levels during the last four time points (Xiong et al, 2002; Figure 3B), while proline, a drought stress-responsive compatible solute in vascular plants (Sperdouli and Moustakas, 2012), accumulated to significant levels only during the last two time points ( Figure 3B). Additionally, the accumulation of secondary metabolites commonly associated with stress responses such as anthocyanins and flavonols were altered during the late stages of the drought response (Sperdouli and Moustakas, 2012; Supplemental Data Sets 1 and 3 and Supplemental Figure 3). Oligosaccharides/disaccharides associated with osmotic protection during drought and osmotic stresses (galactinol and raffinose; Taji et al, 2002) significantly accumulated during the last 4 days of the drought response ( Figure 3C; Supplemental Data Set 1).…”

Section: Metabolite Profiling Indicates the Stable Nature Of Primary mentioning

confidence: 99%

Time-Series Transcriptomics Reveals That AGAMOUS-LIKE22 Affects Primary Metabolism and Developmental Processes in Drought-Stressed Arabidopsis

et al. 2016

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In Arabidopsis thaliana, changes in metabolism and gene expression drive increased drought tolerance and initiate diverse drought avoidance and escape responses. To address regulatory processes that link these responses, we set out to identify genes that govern early responses to drought. To do this, a high-resolution time series transcriptomics data set was produced, coupled with detailed physiological and metabolic analyses of plants subjected to a slow transition from well-watered to drought conditions. A total of 1815 drought-responsive differentially expressed genes were identified. The early changes in gene expression coincided with a drop in carbon assimilation, and only in the late stages with an increase in foliar abscisic acid content. To identify gene regulatory networks (GRNs) mediating the transition between the early and late stages of drought, we used Bayesian network modeling of differentially expressed transcription factor (TF) genes. This approach identified AGAMOUS-LIKE22 (AGL22), as key hub gene in a TF GRN. It has previously been shown that AGL22 is involved in the transition from vegetative state to flowering but here we show that AGL22 expression influences steady state photosynthetic rates and lifetime water use. This suggests that AGL22 uniquely regulates a transcriptional network during drought stress, linking changes in primary metabolism and the initiation of stress responses.

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Interaction of proline, sugars, and anthocyanins during photosynthetic acclimation of Arabidopsis thaliana to drought stress

Cited by 284 publications

References 70 publications

Proline partially overcomes excess molybdenum toxicity in cabbage seedlings grown in vitro

Proline partially overcomes excess molybdenum toxicity in cabbage seedlings grown in vitro

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

Time-Series Transcriptomics Reveals That AGAMOUS-LIKE22 Affects Primary Metabolism and Developmental Processes in Drought-Stressed Arabidopsis

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