Interaction of propidium iodide with graphene oxide and its application for live cell staining

Liu, Fengyu; Gao, Yulong; Hai-ju, LI; Sun, Shiguo

doi:10.1016/j.carbon.2014.01.029

Cited by 16 publications

(14 citation statements)

References 38 publications

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“…This could be because AST attached on the surface of GO sheet is carbonized at a high applied potential difference during the measurement process of FESEM and TEM. 16,17 Taken together, all these results clearly conrm that AST molecules have been successfully transferred from ethyl acetate to water by the formation of a GO-AST complex.…”

mentioning

confidence: 64%

“Pulling” π-conjugated polyene biomolecules into water: enhancement of light-thermal stability and bioactivity by a facile graphene oxide-based phase-transfer approach

Zhang

Liu

et al. 2014

RSC Adv.

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mentioning

confidence: 64%

“Pulling” π-conjugated polyene biomolecules into water: enhancement of light-thermal stability and bioactivity by a facile graphene oxide-based phase-transfer approach

Zhang

Liu

et al. 2014

RSC Adv.

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“…This kind of boron-doping GQD can be further used to detect biomarkers through cellular imaging [139]. Liu et al reported that propidium iodide (PI)-GO can enter into the nuclei and stain the DNA/RNA of living human breast cancer cells MCF-7 [140]. In this study, PI alone without GO cannot cross the cellular membrane, revealing the fact that GO is an efficient PI delivery nanomaterial for live cell staining.…”

Section: Graphene Based Cancer Nanociagnosismentioning

confidence: 82%

Biomarkers-based Biosensing and Bioimaging with Graphene for Cancer Diagnosis

Tang

Xiong

et al. 2019

Nanomaterials

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At the onset of cancer, specific biomarkers get elevated or modified in body fluids or tissues. Early diagnosis of these biomarkers can greatly improve the survival rate or facilitate effective treatment with different modalities. Potential nanomaterial-based biosensing and bioimaging are the main techniques in nanodiagnostics because of their ultra-high selectivity and sensitivity. Emerging graphene, including two dimensional (2D) graphene films, three dimensional (3D) graphene architectures and graphene hybrids (GHs) nanostructures, are attracting increasing interests in the field of biosensing and bioimaging. Due to their remarkable optical, electronic, and thermal properties; chemical and mechanical stability; large surface area; and good biocompatibility, graphene-based nanomaterials are applicable alternatives as versatile platforms to detect biomarkers at the early stage of cancer. Moreover, currently, extensive applications of graphene-based biosensing and bioimaging has resulted in promising prospects in cancer diagnosis. We also hope this review will provide critical insights to inspire more exciting researches to address the current remaining problems in this field.

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“…Cell viability was assessed with nuclei (blue) and dead cell (red) staining, where nuclei signal alone marked live cells and dual staining (seen as magenta) marked dead cells ( ) . Since propidium iodide has been reported to interact with GO sheets[ 66 ], spots with only red staining were disregarded as they correspond to GO-PI complexes. The bioprinted constructs exhibit high cell viability at all measured time points, with a slight decrease on day 3 but a prompt recovery by day 7 ( Figure 6D ).…”

Section: Resultsmentioning

confidence: 99%

Graphene Oxide-Embedded Extracellular MatrixDerived Hydrogel as a Multiresponsive Platform for 3D Bioprinting Applications

Rueda-Gensini

Serna

Cifuentes

et al. 2024

IJB

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Decellularized extracellular matrices (dECMs) have shown enormous potential for the biofabrication of tissues due to their biomimetic properties that promote enhanced cellular interaction and tissue regeneration. However, biofabrication schemes requiring electrostimulation pose an additional constraint due to the insulating properties of natural materials. Here, we propose a methacryloyl-modified decellularized small intestine submucosa (SISMA) hydrogel, embedded with graphene oxide (GO) nanosheets, for extrusion-based 3D bioprinting applications that require electrostimulation. Methacryloyl biochemicalmodification is performed to enhance the mechanical stability of dECM constructs by mediating photo-crosslinking reactions, and a multistep fabrication scheme is proposed to harness the bioactive and hydrophilic properties of GO and electroconductive properties of reduced GO. For this, GO was initially dispersed in SISMA hydrogels by exploiting its hydrophilicity and protein adsorption capabilities, and in situ reduction was subsequently performed to confer electroconductive abilities. SISMA-GO composite hydrogels were successfully prepared with enhanced structural characteristics, as shown by the higher crosslinking degree and increased elastic response upon blue-light exposure. Moreover, GO was homogeneously dispersed without affecting photocrosslinking reactions and hydrogel shear-thinning properties. Human adipose-derived mesenchymal stem cells were successfully bioprinted in SISMA-GO with high cell viability after 1 week and in situ reduction of GO during this period enhanced the electrical conductivity of these nanostructures. This work demonstrates the potential of SISMA-GO bioinks as bioactive and electroconductive scaffolds for electrostimulation applications in tissue engineering and regenerative medicine.

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Interaction of propidium iodide with graphene oxide and its application for live cell staining

Cited by 16 publications

References 38 publications

“Pulling” π-conjugated polyene biomolecules into water: enhancement of light-thermal stability and bioactivity by a facile graphene oxide-based phase-transfer approach

“Pulling” π-conjugated polyene biomolecules into water: enhancement of light-thermal stability and bioactivity by a facile graphene oxide-based phase-transfer approach

Biomarkers-based Biosensing and Bioimaging with Graphene for Cancer Diagnosis

Graphene Oxide-Embedded Extracellular MatrixDerived Hydrogel as a Multiresponsive Platform for 3D Bioprinting Applications

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