Interaction of protein molecules with a network polyelectrolyte in solution

Паутов, В. Д.; Кузнецова, Н. П.; Mishayeva, R.N.; Anufriyeva, Ye.V.

doi:10.1016/0032-3950(83)90303-9

Cited by 5 publications

(2 citation statements)

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“…Systems containing both weak polyelectrolytes and biopolymers have been studied in the context of protein separations: Morawetz (19), Glatz (20), and others (21) studied the phase separation of proteins with homo-and co-polymers of acrylic (AA) and methacrylic acid (MA). Related studies describe soluble complex formation between such polyacids and proteins (22), and binding of proteins to crosslinked acrylic acid copolymers (23). Fleer and coworkers (24) studied the adsorption of PAA onto cationic latexes.…”

Section: Introductionmentioning

confidence: 99%

“…The primary issue in such comparisons has been of course the variable charge density of weak polyelectrolytes and their concomitant pH-dependence, but it has also been pointed out that the pK of ionizable groups of weak polyions may be perturbed by the colloid surface potential. Fleer et al (23) commented on the need to distinguish between the adsorption of strong and weak polyelectrolytes, and found that the effect of ionic strength on adsorption was far lower for weak polyelectrolytes. Shatayeva (33) discussed the relative binding of proteins to weak and strong cation exchange resins in terms of the acid strength of the network ionophores.…”

Section: Introductionmentioning

confidence: 99%

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Binding of Polycarboxylic Acids to Cationic Mixed Micelles: Effects of Polymer Counterion Binding and Polyion Charge Distribution

Yoshida

Sokhakian

Dubin

1998

Journal of Colloid and Interface Science

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Mixed micelles of cetyltrimethylammonium chloride (CTAC) and n-dodecyl hexaoxyethylene glycol monoether (C 12 E 8 ) bind to polyanions when the mole fraction of the cationic surfactant exceeds a critical value (Y c ). Y c corresponds to a critical micelle surface charge density at which polyelectrolyte will bind to this colloidal particle. Turbidimetric titrations were used to determine Y c for such cationic-nonionic micelles in the presence of acrylic acid and acrylamido-2-methylpropane sulfonate homopolymers (PAA and PAMPS, respectively) and their copolymers with acrylamide, as function of pH, ionic strength, and polyelectrolyte counterion. In 0.20 M NaCl, Y c for PAA is found to be remarkably insensitive to pH, i.e., virtually independent of the apparent polymer charge density app . On the other hand, the expected inverse relationship between Y c and app is observed either for PAA when NaCl is replaced by TMACl (tetramethylammonium chloride), or when app is manipulated using acrylic acid/acrylamide copolymers at high pH. The effective charge density of PAA is thus seen to be suppressed by specific sodium ion binding, indicating that the influence of salts on the interaction of polycarboxylic acids with colloidal particles may differ qualitatively from their effect on the analogous behavior of strong polyanions. Comparisons between homo-and copolymers of acrylic acid were carried out also to test the hypothesis that the "mobility" of charges on PAA at moderate pH (degree of ionization less than unity) could make this "annealed" polymer exhibit the behavior of a more highly charged one. The results, while consistent with this expectation, were obscured by the likely effect of copolymer sequence distributions.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%