Interaction of Pseudostellaria heterophylla with Fusarium oxysporum f.sp. heterophylla mediated by its root exudates in a consecutive monoculture system

Ying, Zhao; Wu, Linkun; Chu, Leixia; Yang, Yanqiu; Li, Zhenfang; Azeem, Saadia; Zhang, Zhixing; Fang, Changxun; Lin, Wenxiong

doi:10.1038/srep08197

Cited by 60 publications

(52 citation statements)

References 26 publications

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“…36 Furthermore, FA that was secreted by Pseudostellaria heterophylla roots also stimulated sporulation of the pathogen Fusarium oxysporum f. sp heterophylla and induced plant wilt disease. 12 Previously, our study showed that watermelon wilt disease can be alleviated by rice/watermelon intercropping management. 21 FON is suppressed in vitro by root exudates.…”

Section: Discussionmentioning

confidence: 99%

“…18,19 The accumulation of plant root exudates in the rhizosphere after monoculture causes rapid pathogen proliferation in replanted soil, which negatively impacts soil quality, crop health, and yield. 12,20 Watermelon wilt disease was found to be alleviated by intercropping with aerobic rice. 21 This alleviation was due to suppression of FON pathogen spore germination and sporulation by rice root exudates.…”

Section: Introductionmentioning

confidence: 99%

“…11 The FA secreted by Pseudostellaria heterophylla roots stimulated growth of the pathogen Fusarium oxysporum f. sp heterophylla and induced wilt disease. 12 FA is also reported to serve as a precursor of phenylpropanoid derivatives that have antimicrobial properties and improve plant defense. 13 Salicylic acid (SA) is a phenolic acid that functions as a signal of systemic acquired resistance (SAR) and regulates systemic plant immunity.…”

Section: Introductionmentioning

confidence: 99%

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The components of rice and watermelon root exudates and their effects on pathogenic fungus and watermelon defense

Ren

Huo

Fang

et al. 2016

Plant Signaling & Behavior

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Watermelon (Citrullus lanatus) is susceptible to wilt disease caused by the fungus Fusarium oxysporum f. sp niveum (FON). Intercropping management of watermelon/aerobic rice (Oryza sativa) alleviates watermelon wilt disease, because some unidentified component(s) in rice root exudates suppress FON sporulation and spore germination. Here, we show that the phenolic acid p-coumaric acid is present in rice root exudates only, and it inhibits FON spore germination and sporulation. We found that exogenously applied pcoumaric acid up-regulated the expression of ClPR3 in roots, as well as increased chitinase activity in leaves. Furthermore, exogenously applied p-coumaric acid increased b-1,3-glucanase activity in watermelon roots. By contrast, we found that ferulic acid was secreted by watermelon roots, but not by rice roots, and that it stimulated spore germination and sporulation of FON. Exogenous application of ferulic acid down-regulated ClPR3 expression and inhibited chitinase activity in watermelon leaves. Salicylic acid was detected in both watermelon and rice root exudates, which stimulated FON spore germination at low concentrations and suppressed spore germination at high concentrations. Exogenously applied salicylic acid did not alter ClPR3 expression, but did increase chitinase and b-1,3-glucanase activities in watermelon leaves. Together, our results show that the root exudates of phenolic acids were different between rice and watermelon, which lead to their special ecological roles on pathogenic fungus and watermelon defense.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The components of rice and watermelon root exudates and their effects on pathogenic fungus and watermelon defense

Ren

Huo

Fang

et al. 2016

Plant Signaling & Behavior

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“…Our previous studies by culture-dependent and culture-independent analysis demonstrated that F. oxysporum and other Fusarium spp. are the main causative agents of wilt and rot disease of this plant (Zhao Y. et al, 2015; Wu L. et al, 2016). Our previous studies have demonstrated that the phenolic acids identified in the root exudates of P. heterophylla could significantly inhibit the growth of beneficial Burkholderia sp.…”

Section: Discussionmentioning

confidence: 99%

Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime

Chen

et al. 2016

Front. Microbiol.

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The biomass and quality of Pseudostellariae heterophylla suffers a significant decline under monoculture. Since rhizosphere miobiome plays crucial roles in soil health, deep pyrosequencing combined with qPCR was applied to characterize the composition and structure of soil bacterial community under monoculture and different amendments. The results showed compared with the 1st-year planted (FP), 2nd-year monoculture of P. heterophylla (SP) led to a significant decline in yield and resulted in a significant increase in Fusarium oxysporum but a decline in Burkholderia spp. Bio-organic fertilizer (MT) formulated by combining antagonistic bacteria with organic matter could significantly promote the yield by regulating rhizosphere bacterial community. However, organic fertilizer (MO) without antagonistic bacteria could not suppress Fusarium wilt. Multivariate statistics analysis showed a distinct separation between the healthy samples (FP and MT) and the unhealthy samples (SP and MO), suggesting a strong relationship between soil microbial community and plant performance. Furthermore, we found the application of bio-organic fertilizer MT could significantly increase the bacterial community diversity and restructure microbial community with relatively fewer pathogenic F. oxysporum and more beneficial Burkholderia spp. In conclusion, the application of novel bio-organic fertilizer could effectively suppress Fusarium wilt by enriching the antagonistic bacteria and enhancing the bacterial diversity.

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“…For instance, Pseudomonas catalysed (-)-catechin conversion into 3,4-dihydroxybenzoic acid, and 3,4-dihydroxybenzoic acid showed a higher phytotoxicity than (-)-catechin (Masuda et al 2007); Bacillus aquimaris involved in the degradation of allelopathic m-tyrosine (Khan et al 2013); F. oxysporum, a replant disease pathogen in P. heterophylla rhizospheric soil, increased sharply under a consecutive-replant system, and some phenolic acids promoted the growth of F. oxysporum f.sp. (Zhao et al 2015). In production of R. glutinosa, 15 to 20 years of crop rotation is taken for its replanting (Yang et al 2011b).…”

Section: Discussionmentioning

confidence: 99%

Assaying the potential autotoxins and microbial community associated with Rehmannia glutinosa replant problems based on its ‘autotoxic circle’

Bao

Wang

et al. 2016

Plant Soil

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Aims This study attempted to elucidate the underlying correlations among replant problems of Rehmannia glutinosa, the autotoxins and microbes within its rootzone soil. Methods Different root-zone soils of R. glutinosa were collected to identify the potential autotoxins, to quantify the phenolic acids' concentration and to analyse the microbial community composition. Bioassay was conducted to assess the autotoxic potential of these samples and candidate autotoxins. Results R. glutinosa autotoxicity was presented within a 20 cm radius of the plant. Concentrations of the phenolic acids were significantly higher within this range. Twenty compounds and 17 microbes emerged in this range after the R. glutinosa cultivation. Further seedling test showed that four selected compounds possessed conspicuous autotoxic activity and a synergistic effect was observed. Moreover, one potential beneficial fungi (99 % similar with Arthrobotrys oligospora) and one potential pathogens (100 % similar with Acremonium sclerotigenum) were identified. Conclusions Our work defined the 'autotoxic circle' of R. glutinosa and identified the corresponding autotoxins and microbes. We suggest that R. glutinosa has various autotoxins and that these autotoxins and specific microbes deserve further investigation to unravel their interaction with R. glutinosa in replant problems' occurrence.

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Interaction of Pseudostellaria heterophylla with Fusarium oxysporum f.sp. heterophylla mediated by its root exudates in a consecutive monoculture system

Cited by 60 publications

References 26 publications

The components of rice and watermelon root exudates and their effects on pathogenic fungus and watermelon defense

The components of rice and watermelon root exudates and their effects on pathogenic fungus and watermelon defense

Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime

Assaying the potential autotoxins and microbial community associated with Rehmannia glutinosa replant problems based on its ‘autotoxic circle’

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