Interaction of PvALF and VP1 B3 domains with the β-phaseolin promoter

Carranco, Raúl; Chandrasekharan, Mahesh B.; Townsend, John C.; Hall, Timothy C.

doi:10.1007/s11103-004-0512-8

Cited by 19 publications

(18 citation statements)

References 40 publications

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“…ABI3 activates seed maturation genes, acts upstream of ABI5 (Lopez-Molina et al, 2002) and binds to the Sph/RY (CATGCA) motif (Carranco et al, 2004;Monke et al, 2004;Reidt et al, 2000;Suzuki et al, 1997). This motif was significantly enriched in promoters of the 100 strongest upregulated genes in RBRcs seedlings on sucrose medium (motif occurrence within 500 bp upstream of the start-codon: 57 times, P5.26E-9).…”

Section: Sucrose Causes a Shift Towards Embryonic Identity In Rbrcs Mmentioning

confidence: 98%

RETINOBLASTOMA-RELATED PROTEIN controls the transition to autotrophic plant development

et al. 2011

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SUMMARYSeedling establishment is a crucial phase during plant development when the germinating heterotrophic embryo switches to autotrophic growth and development. Positive regulators of embryonic development need to be turned off, while the cell cycle machinery is activated to allow cell cycle entry and organ primordia initiation. However, it is not yet understood how the molecular mechanisms responsible for the onset of cell division, metabolism changes and cell differentiation are coordinated during this transition. Here, we demonstrate that the Arabidopsis thaliana RETINOBLASTOMA-RELATED protein (RBR) ortholog of the animal tumor suppressor retinoblastoma (pRB) not only controls the expression of cell cycle-related genes, but is also required for persistent shut-down of late embryonic genes by increasing their histone H3K27 trimethylation. Seedlings with reduced RBR function arrest development after germination, and stimulation with low amounts of sucrose induces transcription of late embryonic genes and causes ectopic cell division. Our results suggest a model in which RBR acts antagonistically to sucrose by negatively regulating the cell cycle and repressing embryonic genes. Thus, RBR is a positive regulator of the developmental switch from embryonic heterotrophic growth to autotrophic growth. This establishes RBR as a new integrator of metabolic and developmental decisions.

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Section: Sucrose Causes a Shift Towards Embryonic Identity In Rbrcs Mmentioning

confidence: 98%

RETINOBLASTOMA-RELATED PROTEIN controls the transition to autotrophic plant development

et al. 2011

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“…LEC2, FUS3, and ABI3 activation of downstream maturation genes is mediated in part by specific binding of the B3 domain to Sph/RY cis-elements (Suzuki et al, 1997;Kroj et al, 2003;Carranco et al, 2004;Monke et al, 2004;Braybrook et al, 2006). Because the VAL B3 domain is closely related, there is potential for direct interaction with downstream genes as well as indirect regulation via derepression of the B3 activators.…”

Section: Val Is Required For Global Repression Of Embryonic Gene Exprmentioning

confidence: 99%

“…Activation of downstream genes is mediated by specific binding of the B3 domain (Suzuki et al, 1997;Kroj et al, 2003;Carranco et al, 2004;Monke et al, 2004;Braybrook et al, 2006) to the Sph/RY cis-element (Hattori et al, 1992;Kao et al, 1996;Ezcurra et al, 1999;Reidt et al, 2000;Chandrasekharan et al, 2003;Nag et al, 2005). In addition, ABI3 mediates ABA-regulated gene expression in the seed through interaction with specific basic Leu-zipper transcription factors that bind ABA response elements (Finkelstein et al, 2002).…”

mentioning

confidence: 99%

Repression of theLEAFY COTYLEDON 1/B3Regulatory Network in Plant Embryo Development byVP1/ABSCISIC ACID INSENSITIVE 3-LIKEB3 Genes

2006

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Plant embryo development is regulated by a network of transcription factors that include LEAFY COTYLEDON 1 (LEC1), LEC1-LIKE (L1L), and B3 domain factors, LEAFY COTYLEDON 2 (LEC2), FUSCA3 (FUS3), and ABSCISIC ACID INSENSITIVE 3 (ABI3) of Arabidopsis (Arabidopsis thaliana). Interactions of these genes result in temporal progression of overlapping B3 gene expression culminating in maturation and desiccation of the seed. Three VP1/ABI3-LIKE (VAL) genes encode B3 proteins that include plant homeodomain-like and CW domains associated with chromatin factors. Whereas val monogenic mutants have phenotypes similar to wild type, val1 val2 double-mutant seedlings form no leaves and develop embryo-like proliferations in root and apical meristem regions. In a val1 background, val2 and val3 condition a dominant variegated leaf phenotype revealing a VAL function in vegetative development. Reminiscent of the pickle (pkl) mutant, inhibition of gibberellin biosynthesis during germination induces embryonic phenotypes in val1 seedlings. Consistent with the embryonic seedling phenotype, LEC1, L1L, ABI3, and FUS3 are up-regulated in val1 val2 seedlings in association with a global shift in gene expression to a profile resembling late-torpedo-stage embryogenesis. Hence, VAL factors function as global repressors of the LEC1/B3 gene system. The consensus binding site of the ABI3/FUS3/LEC2 B3 DNA-binding domain (Sph/RY) is strongly enriched in the promoters and first introns of VAL-repressed genes, including the early acting LEC1 and L1L genes. We suggest that VAL targets Sph/RY-containing genes in the network for chromatin-mediated repression in conjunction with the PKLrelated CHD3 chromatin-remodeling factors.

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“…DNA purified after immunoprecipitation was evaluated by PCR using primers targeting the proximal region of the phas promoter. The targeted 227-bp amplicon ( Figure 3) includes four RY elements (the binding motif for ALF) (Carranco et al, 2004) and a G-box (ABA response element [ABRE]) (Ezcurra et al, 2000) that are essential for phas expression (Chandrasekharan et al, 2003a). As an internal control, primers targeting the 59 end (133 bp) of actin7 (McDowell et al, 1996) were used for PCR.…”

Section: Potentiation and Activation Of Phas Can Be Temporally Separatedmentioning

confidence: 99%

“…This complex contains three transcription factors (NF-kB, IRFs, and ATF-2/c-Jun) and HMG I(Y), an architectural protein that binds to specific sites in the nucleosome-free enhancer DNA, altering its topology and decreasing the free energy for activator binding (Falvo et al, 1995). In the phas system, ALF is known to be required for nucleosome remodeling (Li et al, 1998 and binds to RY motifs (Carranco et al, 2004) present within a 68-bp seed-specific enhancer sequence (van der Geest and Hall, 1996); it thus appears to be functionally similar to the architectural protein of the human IFN-b system.…”

Section: Similarities In Histone Code Functions Among Eukaryotic Systemsmentioning

confidence: 99%

Ordered Histone Modifications Are Associated with Transcriptional Poising and Activation of the phaseolin Promoter

Chandrasekharan

Hall

2005

Plant Cell

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The phaseolin (phas) promoter drives copious production of transcripts encoding the protein phaseolin during seed embryogenesis but is silent in vegetative tissues, in which a nucleosome is positioned over its three-phased TATA boxes. Transition from the inactive state in transgenic Arabidopsis thaliana leaves was accomplished by ectopic expression of the transcription factor Phaseolus vulgaris ABI3-like factor (ALF) and application of abscisic acid (ABA). Placement of hemagglutinin-tagged ALF expression under the control of an estradiol-inducible promoter permitted chromatin immunoprecipitation analysis of chronological changes in histone modifications, notably increased acetylation of H3-K9 and H4-K12, as phas chromatin was remodeled (potentiated). A different array of changes, including acetylation of H3-K14 and methylation of H3-K4, was found to be associated with ABA-mediated activation. Thus, temporal separation of phas potentiation from activation revealed that histone H3 and H4 Lys residues are not globally hyperacetylated during phas expression. Whereas decreases in histone H3 and H4 levels were detected during ALF-mediated remodeling, slight increases occurred after ABA-mediated activation, suggesting the restoration of histone-phas interactions or the replacement of histones in the phas chromatin. The observed histone modifications provide insight into factors involved in the euchromatinization and activation of a plant gene and expand the evidence for histone code conservation among eukaryotes.

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Interaction of PvALF and VP1 B3 domains with the β-phaseolin promoter

Cited by 19 publications

References 40 publications

RETINOBLASTOMA-RELATED PROTEIN controls the transition to autotrophic plant development

RETINOBLASTOMA-RELATED PROTEIN controls the transition to autotrophic plant development

Repression of theLEAFY COTYLEDON 1/B3Regulatory Network in Plant Embryo Development byVP1/ABSCISIC ACID INSENSITIVE 3-LIKEB3 Genes

Ordered Histone Modifications Are Associated with Transcriptional Poising and Activation of the phaseolin Promoter

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