Interaction of sporozoites of Theileria parva with bovine lymphocytes in vitro. 1. Early events after invasion

Fawcett, Don W.; Musoke, A.J.; Voigt, W. P.

doi:10.1016/0040-8166(84)90068-5

Cited by 63 publications

(59 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recess developed on the endothelial cell surface, and the parasite moved into the host cell with the host cell membrane curving around the invading parasite, which eventually became intracellular, completely encircled by a parasitophorous vacuole membrane. This process is likely to be very similar, but not necessarily identical, to the 'self zippering mechanism' described for the invasion of host cells by other intracellular parasites such as T. cruzi (Schenkman et al 1991a; Theileria parva (Fawcett et al 1984), Plasmodium spp. (Tait & Sacks;Holder, 1994), and Toxoplasma gondii (Werk, 1985;Bonhomme et al 1992).…”

Section: Discussionmentioning

confidence: 67%

Adhesion and invasion of bovine endothelial cells byNeospora caninum

Gottstein²,

1996

View full text Add to dashboard Cite

SUMMARYNeospora caninum is a recently identified coccidian parasite which was, until 1988, misdiagnosed as Toxoplasma gondii. It causes paralysis and death in dogs and neonatal mortality and abortion in cattle, sheep, goats and horses. The life-cycle of Neospora has not yet been elucidated. The only two stages identified so far are tissue cysts and intracellularly dividing tachyzoites. Very little is known about the biology of this species. We have set up a fluorescence-based adhesion / invasion assay in order to investigate the interaction of N. caninum tachyzoites with bovine aorta endothelial (BAE) cells in vitro. Treatment of both host cells and parasites with metabolic inhibitors determined the metabolic requirements for adhesion and invasion. Chemical and enzymatic modifications of parasite and endothelial cell surfaces were used in order to obtain information on the nature of cell surface components responsible for the interaction between parasite and host. Electron microscopical investigations defined the ultrastructural characteristics of the adhesion and invasion process, and provided information on the intracellular development of the parasites.

show abstract

Section: Discussionmentioning

confidence: 67%

Adhesion and invasion of bovine endothelial cells byNeospora caninum

Gottstein²,

1996

View full text Add to dashboard Cite

show abstract

“…Theileria sporozoites also deviate from this pattern. Although the tick-borne kinete stage of Theileria contains subpellicular microtubules and an IMC, Theileria sporozoites lack subpellicular microtubules and the IMC altogether and enter host cells in a distinct fashion (see below) (49,50,147,148,152).…”

Section: Microtubules In the Apicomplexa Organizationmentioning

confidence: 99%

“…Theileria sporozoites are bounded by a simple plasma membrane structure and lack both an inner membrane complex and subpellicular microtubules. Host cell entry does not require apical orientation of the Theileria sporozoite and occurs by a zippering rather than a moving-junction mechanism (49,50,79,147,148,152). Once inside the host cell, the parasite escapes from the parasitophorous vacuole and takes up residence in the cytoplasm.…”

Section: Theileria Exploitation Of Host Cell Microtubulesmentioning

confidence: 99%

“…Once inside the host cell, the parasite escapes from the parasitophorous vacuole and takes up residence in the cytoplasm. The parasite plasma membrane becomes coated with a number of host cell-derived microtubules organized in arrays tangential to the sporozoite surface (49,79,117,147,175). Sporozoite-associated microtubules are highly resistant to nocodazole disruption (147,175).…”

Section: Theileria Exploitation Of Host Cell Microtubulesmentioning

confidence: 99%

See 1 more Smart Citation

Cytoskeleton of Apicomplexan Parasites

Morrissette

Sibley

2002

Microbiol Mol Biol Rev

414

402

View full text Add to dashboard Cite

The Apicomplexa are a phylum of diverse obligate intracellular parasites including Plasmodium spp., the cause of malaria; Toxoplasma gondii and Cryptosporidium parvum, opportunistic pathogens of immunocompromised individuals; and Eimeria spp. and Theileria spp., parasites of considerable agricultural importance. These protozoan parasites share distinctive morphological features, cytoskeletal organization, and modes of replication, motility, and invasion. This review summarizes our current understanding of the cytoskeletal elements, the properties of cytoskeletal proteins, and the role of the cytoskeleton in polarity, motility, invasion, and replication. We discuss the unusual properties of actin and myosin in the Apicomplexa, the highly stereotyped microtubule populations in apicomplexans, and a network of recently discovered novel intermediate filament-like elements in these parasites

show abstract

“…This interaction includes host cell signaling pathways that regulate proliferation and cell survival (Chaussepied and Langsley 2011) and thus cause blastogenesis and clonal expansion of predominantly T and B cells (Fawcett et al 1982, Baldwin et al 1988, Spooner et al 1989. Merozoites released from these schizonts subsequently infect red blood cells and become trophozoites.…”

Section: Introductionmentioning

confidence: 99%

Targeting tams-1 gene results in underestimation of Theileria annulata infection in diseased cattle in Egypt

Ghoneim

El-Fayomy

2014

Acta Parasitologica

View full text Add to dashboard Cite

Tropical theileriosis is considered one of the most economically important tick borne diseases that cause significant mortality and morbidity to livestock. In the context of epidemiological studies on livestock in Egypt, this report investigated the spread of Theileria annulata among diseased farm cows (Bos indicus) over one year. Blood samples collected from 130 cows were investigated by routine staining and 64 samples were investigated by PCR assay using two different probes targeting tams-1 gene. Microscopy revealed the infection of 33.8% of animals with Theileria while PCR detected infection in 51% of animals with one primer pair and the other primer pair detected the infection in 31% of animals. Combined PCR results indicated the infection of 68.8% of animals with T. annulata. Seasonal fluctuation of parasite infection was evident with the highest infection percentage and prevalence recorded during summer based on both microscopy and PCR data. For the first time, the current study reports the presence of two T. annulata isolates based on tams-1 gene partial sequence in Egypt. Targeting polymorphic genes for parasite detection may result in underestimation of infection and target gene diversity has to be considered. The high infection with these pathogens in the clinically ill cows necessitates implementing serious programs to minimize their economic burden on the Egyptian farming industry.

show abstract

Interaction of sporozoites of Theileria parva with bovine lymphocytes in vitro. 1. Early events after invasion

Cited by 63 publications

References 7 publications

Adhesion and invasion of bovine endothelial cells byNeospora caninum

Adhesion and invasion of bovine endothelial cells byNeospora caninum

Cytoskeleton of Apicomplexan Parasites

Targeting tams-1 gene results in underestimation of Theileria annulata infection in diseased cattle in Egypt

Contact Info

Product

Resources

About