Interaction of Subculture Cycle, Hormone Ratio, and Carbon Source Regulates Embryonic Differentiation of Somatic Cells in Pinus koraiensis

Ren, Yuhui; Yu, Xiaoqian; Xing, Honglin; Tretyakova, I. N.; Носов, А. М.; Yang, Ling; Shen, Hailong

doi:10.3390/f13101557

Cited by 8 publications

(9 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the experimental group with a higher number of somatic embryo transitions also showed higher SOD activity, which supporting previous results. As the subculture cycle increased, SOD activity in the plants decreased significantly, which is consistent with the studies of Ren et al. (2022) .…”

Section: Discussionsupporting

confidence: 90%

“…In somatic embryogenesis of Camellia sinensis ‘Tieguanyin’, the content of endogenous IAA and ABA was found to be closely related to the development of spherical embryos and the maturation of somatic embryos ( Guo et al., 2018 ). In addition, the ratio of IAA to ABA is a crucial factor influencing somatic embryogenesis in plants ( Ren et al., 2022 ). The expression of callus embryogenicity benefits from a lower ratio of IAA: ABA ( Liang et al., 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…(2011) . To counteract this, one must intervene with exogenous hormones to reduce the embryogenic loss of embryogenic callus or improve the stress resistance of embryogenic callus to maintain the number of embryogenic callus ( Ren et al., 2022 ). This approach offers a solution to the problem of pine SE, as the specific steps by which this problem occurs have been identified.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Proper doses of brassinolide enhance somatic embryogenesis in different competent Korean pine cell lines during embryogenic callus differentiation

Nie,

Yan,

Wang

et al. 2024

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Somatic embryogenesis of Korean pine (Pinus koraiensis Sieb. Et Zucc.), an ecologically and econimically very important conifer species, was hindered by the gradually weakens and fast runaway of the embryogenicity and embryo competence of the embryogenic callus. Brassinolide (BL) has shown the enhancing capability of somatic embryo regeneration. For checking the function of BL in this issue, we applied different concentrations of BL to Korean pine callus materials exhibiting different embryogenic capacities and subsequently monitored the physiological alterations and hormone dynamics of the embryogenic callus. Our study revealed that calli with different embryogenic strengths responded differently to different concentrations of BL, but the effect after the addition of BL was very uniform. The addition of BL during the proliferation phase of embryogenic callus may help to stimulate the biological activity of callus during the proliferation process and improve the level of cell metabolism, which is accompanied by a reduction in storage substances. BL could reduce the level of endogenous auxin IAA in embryogenic callus and increase the level of abscisic acid to regulate cell division and differentiation. In addition, the MDA content in the callus was significantly decreased and the activity of antioxidant enzymes was significantly increased after the addition of BL. During the proliferation of embryogenic callus, BL was added to participate in the metabolism of phenylpropane in the cells and to increase the activity of phenylalanine ammonia-lyase and the content of lignin in the cells. We deduced that the proper doses of BL for Korean pine embryogenic callus culture were as follow: calli with low, high and decreasing embryogenicity were subcultured after the addition of 0.75 mg/L, 0.35 mg/L, 2.00 mg/L BL, respectively, during proliferation culture stage.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Proper doses of brassinolide enhance somatic embryogenesis in different competent Korean pine cell lines during embryogenic callus differentiation

Nie,

Yan,

Wang

et al. 2024

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In green budwood culture, low concentration of BA at 0.5 mg/L and high concentration of 2,4-D at 2.0 mg/L required, plantlet regeneration in this study required high concentration of BA (2.0 mg/L) and slightly lower concentration of 2,4-D. This might be due to different amount of plant growth regulators and the respond to it.In addition, several researchers reported that MS medium supplemented with 0.06 mg/L NAA and 0.03 mg/L BA gave the suitable for SE induction and plant conversion rate in many plant species including Canabis sativa L. [16], Cinchona officinalis [18] and Pinus koraiensis [29]. The low level of auxin and cytokinin in the SE induction medium was sometimes responsible for the development of SE directly from the explants, implying that they did not develop through callus formation [30].…”

Section: Effect Of Types Of Explants On Ec Formation and Number Of Sesmentioning

confidence: 99%

Somatic Embryo (SE) Formation from Culturing Floral Explants of Rubber Tree (Hevea brasiliensis Muell. Arg.) and Assessment of Genetic Stability by RAPD and SSR Markers

Tongtape,

Te-chato,

Yenchon

2023

Trends Sci

View full text Add to dashboard Cite

Rubber tree is economically important rubber producing plant of Thailand. At present, a rubber tree plantation is susceptible to white root disease. Therefore, the use of rootstock from early introduce clone that proved to be resistant to white root disease could help sustain growing of rubber tree. Thus, the objectives of this research were to study the effects of plant growth regulators and different explants on somatic embryo (SE) induction of this rubber clone and assessment genetic stability. The results revealed that mix flower explant cultured on MS medium supplemented with 2.0 mg/L 6-benzyladenine (BA) and 1.5 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) provided callus subsequent somatic embryo (SE) formation at the highest frequency of 39.84 % and number of cotyledonary embryos (CEs) at 3.25 embryos /callus after 3 passages of subculture in the same culture medium (4 weeks/passage). SEs germinated into embryonic axis at 50 % and further development into shoot at 25 % after transfer to 0.25 mg/L GA3 containing MS medium with the best concentrations of BA and 2,4-D for 4 weeks. The assessment gene stability by RAPD and SSR markers showed no variation between mother plant and in vitro plantlets. In this work, a novel explant source - the floral section of the rubber tree - was used for the first time in order to design an effective technique for in vitro somatic embryogenesis. HIGHLIGHTS The first report on plantlets regeneration using floral explant of rubber tree This mother plant is proved to be resistance to Phytopthora leaf fall and white root diseases Assessment genetic stability of regenerated plantlets by SSR and RAPD markers GRAPHICAL ABSTRACT

show abstract

“…Ecological and economic value may be found in the monoecious plant Pinus koraiensis , an evergreen conifer of Pinaceae with a tree height of 40 m and a breast height diameter of 1 m ( Li et al., 2022 ; Park et al., 2016 ; Ren et al., 2022 ). P. koraiensis is mostly distributed in China’s Changbai Mountains, Laoyeling Mountains, Zhangguangcailing Mountains, Xiaoxinganling Mountains and Wanda Mountains, as well as in Japan, the Far East region of Russia and the Korean Peninsula ( Aizawa et al., 2012 ; Shi et al., 2020 ; Wei et al, 2022 ; Zhang et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Phytohormone biosynthesis and transcriptional analyses provide insight into the main growth stage of male and female cones Pinus koraiensis

Li,

Zhao,

Cai

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

The cone is a crucial component of the whole life cycle of gymnosperm and an organ for sexual reproduction of gymnosperms. In Pinus koraiensis, the quantity and development process of male and female cones directly influence seed production, which in turn influences the tree’s economic value. There are, however, due to the lack of genetic information and genomic data, the morphological development and molecular mechanism of female and male cones of P. koraiensis have not been analyzed. Long-term phenological observations were used in this study to document the main process of the growth of both male and female cones. Transcriptome sequencing and endogenous hormone levels at three critical developmental stages were then analyzed to identify the regulatory networks that control these stages of cones development. The most significant plant hormones influencing male and female cones growth were discovered to be gibberellin and brassinosteroids, according to measurements of endogenous hormone content. Additionally, transcriptome sequencing allowed the identification of 71,097 and 31,195 DEGs in male and female cones. The synthesis and control of plant hormones during cones growth were discovered via enrichment analysis of key enrichment pathways. FT and other flowering-related genes were discovered in the coexpression network of flower growth development, which contributed to the growth development of male and female cones of P. koraiensis. The findings of this work offer a cutting-edge foundation for understanding reproductive biology and the molecular mechanisms that control the growth development of male and female cones in P. koraiensis.

show abstract

Interaction of Subculture Cycle, Hormone Ratio, and Carbon Source Regulates Embryonic Differentiation of Somatic Cells in Pinus koraiensis

Cited by 8 publications

References 27 publications

Proper doses of brassinolide enhance somatic embryogenesis in different competent Korean pine cell lines during embryogenic callus differentiation

Proper doses of brassinolide enhance somatic embryogenesis in different competent Korean pine cell lines during embryogenic callus differentiation

Somatic Embryo (SE) Formation from Culturing Floral Explants of Rubber Tree (Hevea brasiliensis Muell. Arg.) and Assessment of Genetic Stability by RAPD and SSR Markers

Phytohormone biosynthesis and transcriptional analyses provide insight into the main growth stage of male and female cones Pinus koraiensis

Contact Info

Product

Resources

About