Interaction of the<i>Neurospora crassa</i>heat shock factor with the heat shock element during heat shock and different developmental stages

Meyer, Ulf; Monnerjahn, Christian; Techel, Dieter; Rensing, Ludger

doi:10.1111/j.1574-6968.2000.tb09071.x

Cited by 9 publications

(4 citation statements)

References 20 publications

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“…Furthermore, small HSPs, such as HSP26 and HSP42, play a vital role in promoting protein solubility when HS leads to general cytosolic protein unfolding (21). In most fungi, the increased expression of HSPs in a stressed cell is mediated primarily by heat shock factors (HSFs) (22).…”

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confidence: 99%

Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca ²⁺

Zhang

Ren

et al. 2016

Appl Environ Microbiol

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Heat stress (HS) influences the growth and development of organisms. Thus, a comprehensive understanding of how organisms sense HS and respond to it is required. Ganoderma lucidum, a higher basidiomycete with bioactive secondary metabolites, has become a potential model system due to the complete sequencing of its genome, transgenic systems, and reliable reverse genetic tools. In this study, we found that HS inhibited mycelium growth, reduced hyphal branching, and induced the accumulation of ganoderic acid biosynthesis and heat shock proteins (HSPs) in G. lucidum. Our data showed that HS induced a significant increase in cytosolic Ca 2؉ concentration. Further evidence showed that Ca 2؉ might be a factor in the HS-mediated regulation of hyphal branching, ganoderic acid (GA) biosynthesis, and the accumulation of HSPs. Our results further showed that the calcium-permeable channel gene (cch)-silenced and phosphoinositide-specific phospholipase gene (plc)-silenced strains reduced the HS-induced increase in HSP expression compared with that observed for the wild type (WT). This study demonstrates that cytosolic Ca 2؉ participates in heat shock signal transduction and regulates downstream events in filamentous fungi. IMPORTANCEGanoderma lucidum, a higher basidiomycete with bioactive secondary metabolites, has become a potential model system for evaluating how environmental factors regulate the development and secondary metabolism of basidiomycetes. Heat stress (HS) is an important environmental challenge. In this study, we found that HS inhibited mycelium growth, reduced hyphal branching, and induced HSP expression and ganoderic acid biosynthesis in G. lucidum. Further evidence showed that Ca 2؉ might be a factor in the HS-mediated regulation of hyphal branching, GA biosynthesis, and the accumulation of HSPs. This study demonstrates that cytosolic Ca 2؉ participates in heat shock signal transduction and regulates downstream events in filamentous fungi. Our research offers a new way to understand the mechanism underlying the physiological and metabolic responses to other environmental factors in G. lucidum. This research may also provide the basis for heat shock signal transduction studies of other fungi. Ganoderma lucidum, a traditional precious medicinal mushroom, has been commonly used throughout China and Southeast Asia for many centuries as a home remedy for treating minor disorders and promoting vitality and longevity (1). Modern pharmacological and clinical research has demonstrated that G. lucidum has significant antitumor, antiviral, antihypertensive, and immunomodulatory activities (2, 3). These pharmaceutical activities come from the bioactive compounds of G. lucidum. In recent years, many of these biologically useful compounds, including ganoderic acids (GAs) and polysaccharides, have been isolated and characterized in G. lucidum (4,5). Ganoderic acids, also called triterpenoids, are one of the major secondary metabolites with pharmacological activity and are also known to be an important medicinal i...

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confidence: 99%

Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca ²⁺

Zhang

Ren

et al. 2016

Appl Environ Microbiol

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“…Despite the lack of nucleotide-resolution studies characterizing these hse as functional, their sequence identity strongly suggests that these regions are conserved and are likely recognized by HSFs, commanded by the major regulator in Neurospora: HSF-1 (NCU08512). In addition, it has been shown that the HSF of Neurospora can efficiently recognize a hse from yeast [81]. Based on this, we used one of the HSF-1 motifs obtained from yeast and N. crassa to further identify new, and confirm the previously proposed hse in the hsp30 promoter (Tabla S2, S3).…”

Section: Discussionmentioning

confidence: 74%

Developing a temperature-inducible transcriptional rheostat inNeurospora crassa

Tabilo-Agurto¹,

Rio-Pinilla²,

Eltit-Villarroel³

et al. 2022

Preprint

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Heat shock protein (hsp) encoding genes, part of the highly conserved Heat Shock Response (HSR), are known to be induced by thermal stress in several organisms. In Neurospora crassa, three hsp genes, hsp30, hsp70, and hsp80, have been characterized; however, the role of defined cis-elements in their response to discrete changes in temperature remains largely unexplored. To fill this gap, while also aiming to obtain a reliable fungal heat-shock inducible system, we analyzed different sections of each hsp promoter, by assessing the expression of real-time transcriptional reporters. Whereas all three promoters, and their resected versions, were acutely induced by high temperatures, only hsp30 displayed a broad range of expression and high tunability amply exciding other inducible promoter systems existing in Neurospora, such as Quinic acid- or light-inducible ones. As proof of concept, we employed one of these promoters to control the expression of clr-2, which encodes for the master regulator of Neurospora cellulolytic capabilities. The resulting strain fails to grow on cellulose at 25˚C, whereas it robustly grows if heat shock pulses are delivered daily. Additionally, we designed two hsp30 synthetic promoters and characterized these, as well as the native promoters, to a gradient of high temperatures, yielding a wide range of responses to thermal stimuli. Thus, Neurospora hsp30-based promoters represent a new set of modular elements that can be used as a transcriptional rheostat to adjust the expression of a gene of interest or for the implementation of regulated circuitries for synthetic biology and biotechnological strategies.

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“…Despite the lack of nucleotide resolution studies characterizing these hse as functional, their sequence identity strongly suggests that these regions are conserved and are likely recognized by HSFs, commanded by the major regulator in Neurospora , HSF-1 (NCU08512). In addition, it has been shown that the HSF of Neurospora can efficiently recognize an hse from yeast ( 81 ). Based on this, we used one of the HSF-1 motifs obtained from yeast and N. crassa to further identify new hse and confirm the previously proposed hse in the hsp30 promoter (Table S1 at https://doi.org/10.6084/m9.figshare.21774671 and Table S2 at https://doi.org/10.6084/m9.figshare.21774632 ).…”

Section: Discussionmentioning

confidence: 99%

Developing a Temperature-Inducible Transcriptional Rheostat in Neurospora crassa

Tabilo-Agurto

Rio-Pinilla

Eltit-Villarroel

et al. 2023

mBio

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Interaction of theNeurospora crassaheat shock factor with the heat shock element during heat shock and different developmental stages

Cited by 9 publications

References 20 publications

Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca ²⁺

Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca ²⁺

Developing a temperature-inducible transcriptional rheostat inNeurospora crassa

Developing a Temperature-Inducible Transcriptional Rheostat in Neurospora crassa

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Interaction of theNeurospora crassaheat shock factor with the heat shock element during heat shock and different developmental stages

Cited by 9 publications

References 20 publications

Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca 2+

Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca 2+

Developing a temperature-inducible transcriptional rheostat inNeurospora crassa

Developing a Temperature-Inducible Transcriptional Rheostat in Neurospora crassa

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Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca ²⁺

Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca ²⁺