Interaction of the Neurospora crassa heat shock factor with the heat shock element during heat shock and different developmental stages

Meyer, Ulf

doi:10.1016/s0378-1097(00)00105-1

Cited by 4 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For Neurospora, HSE are not yet well defined, but nGAAn .. nTTCn motifs (where "n" can be any nucleotide) are present in the promoters of hsp30 (38) and hsp70 (21). Additionally, a factor in Neurospora protein extracts binds specifically to labeled oligonucleotides containing the classic yeast HSF binding sequence nTTCnnGAAnnTTCn (32). Band shift assays with purified Neurospora HSF(s) should aid in the identification of the discriminating consensus binding sites for HSF1 and HSF2.…”

Section: Discussionmentioning

confidence: 99%

Neurospora crassa heat shock factor 1 Is an Essential Gene; a Second Heat Shock Factor-Like Gene, hsf2 , Is Required for Asexual Spore Formation

et al. 2008

View full text Add to dashboard Cite

Appropriate responses of organisms to heat stress are essential for their survival. In eukaryotes, adaptation to high temperatures is mediated by heat shock transcription factors (HSFs). HSFs regulate the expression of heat shock proteins, which function as molecular chaperones assisting in protein folding and stability. In many model organisms a great deal is known about the products of hsf genes. An important exception is the filamentous fungus and model eukaryote Neurospora crassa. Here we show that two Neurospora crassa genes whose protein products share similarity to known HSFs play different biological roles. We report that heat shock factor 1 (hsf1) is an essential gene and that hsf2 is required for asexual development. Conidiation may be blocked in the hsf2 knockout (hsf2 KO ) strain because HSF2 is an integral element of the conidiation pathway or because it affects the availability of protein chaperones. We report that genes expressed during conidiation, for example fluffy, conidiation-10, and repressor of conidiation-1 show wild-type levels of expression in a hsf2 KO strain. However, consistent with the lack of macroconidium development, levels of eas are much reduced. Cultures of the hsf2 KO strain along with two other aconidial strains, the fluffy and aconidial-2 strains, took longer than the wild type to recover from heat shock. Altered expression profiles of hsp90 and a putative hsp90-associated protein in the hsf2 KO strain after exposure to heat shock may in part account for its reduced ability to cope with heat stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Neurospora crassa heat shock factor 1 Is an Essential Gene; a Second Heat Shock Factor-Like Gene, hsf2 , Is Required for Asexual Spore Formation

et al. 2008

View full text Add to dashboard Cite

show abstract

“…The HSF of N. crassa has been shown to bind constitutively to HSEs under nonstress conditions while heat shock induced an alteration in HSF which led to its fast inactivation (Meyer et al , 2000; Monnerjahn et al , 2001).…”

Section: Hsps and Nonpathogenic Fungimentioning

confidence: 99%

Fungal heat-shock proteins in human disease

Burnie¹,

Carter²,

Hodgetts³

et al. 2006

FEMS Microbiol Rev

135

108

View full text Add to dashboard Cite

Heat-shock proteins (hsps) have been identified as molecular chaperones conserved between microbes and man and grouped by their molecular mass and high degree of amino acid homology. This article reviews the major hsps of Saccharomyces cerevisiae, their interactions with trehalose, the effect of fermentation and the role of the heat-shock factor. Information derived from this model, as well as from Neurospora crassa and Achlya ambisexualis, helps in understanding the importance of hsps in the pathogenic fungi, Candida albicans, Cryptococcus neoformans, Aspergillus spp., Histoplasma capsulatum, Paracoccidioides brasiliensis, Trichophyton rubrum, Phycomyces blakesleeanus, Fusarium oxysporum, Coccidioides immitis and Pneumocystis jiroveci. This has been matched with proteomic and genomic information examining hsp expression in response to noxious stimuli. Fungal hsp90 has been identified as a target for immunotherapy by a genetically recombinant antibody. The concept of combining this antibody fragment with an antifungal drug for treating life-threatening fungal infection and the potential interactions with human and microbial hsp90 and nitric oxide is discussed.

show abstract

“…Despite the lack of nucleotide-resolution studies characterizing these hse as functional, their sequence identity strongly suggests that these regions are conserved and are likely recognized by HSFs, commanded by the major regulator in Neurospora : HSF-1 (NCU08512). In addition, it has been shown that the HSF of Neurospora can efficiently recognize a hse from yeast [81]. Based on this, we used one of the HSF-1 motifs obtained from yeast and N. crassa to further identify new, and confirm the previously proposed hse in the hsp30 promoter (Tabla S2, S3).…”

Section: Discussionmentioning

confidence: 63%

Developing a temperature-inducible transcriptional rheostat inNeurospora crassa

Tabilo-Agurto¹,

Rio-Pinilla²,

Eltit-Villarroel³

et al. 2022

Preprint

View full text Add to dashboard Cite

Heat shock protein (hsp) encoding genes, part of the highly conserved Heat Shock Response (HSR), are known to be induced by thermal stress in several organisms. In Neurospora crassa, three hsp genes, hsp30, hsp70, and hsp80, have been characterized; however, the role of defined cis-elements in their response to discrete changes in temperature remains largely unexplored. To fill this gap, while also aiming to obtain a reliable fungal heat-shock inducible system, we analyzed different sections of each hsp promoter, by assessing the expression of real-time transcriptional reporters. Whereas all three promoters, and their resected versions, were acutely induced by high temperatures, only hsp30 displayed a broad range of expression and high tunability amply exciding other inducible promoter systems existing in Neurospora, such as Quinic acid- or light-inducible ones. As proof of concept, we employed one of these promoters to control the expression of clr-2, which encodes for the master regulator of Neurospora cellulolytic capabilities. The resulting strain fails to grow on cellulose at 25˚C, whereas it robustly grows if heat shock pulses are delivered daily. Additionally, we designed two hsp30 synthetic promoters and characterized these, as well as the native promoters, to a gradient of high temperatures, yielding a wide range of responses to thermal stimuli. Thus, Neurospora hsp30-based promoters represent a new set of modular elements that can be used as a transcriptional rheostat to adjust the expression of a gene of interest or for the implementation of regulated circuitries for synthetic biology and biotechnological strategies.

show abstract

Interaction of the Neurospora crassa heat shock factor with the heat shock element during heat shock and different developmental stages

Cited by 4 publications

References 18 publications

Neurospora crassa heat shock factor 1 Is an Essential Gene; a Second Heat Shock Factor-Like Gene, hsf2 , Is Required for Asexual Spore Formation

Neurospora crassa heat shock factor 1 Is an Essential Gene; a Second Heat Shock Factor-Like Gene, hsf2 , Is Required for Asexual Spore Formation

Fungal heat-shock proteins in human disease

Developing a temperature-inducible transcriptional rheostat inNeurospora crassa

Contact Info

Product

Resources

About