Interaction of the Protein C Activation Peptide with Platelets

Cruz, Margarito Martínez; Canseco, María del Socorro Pina; Lopez-Martínez, Jael; Cruz, Pedro António Hernández; Pérez-Campos, Eduardo; Alva, Félix Córdoba; Majluf‐Cruz, Abraham; Zenteno, Edgar; Ruı́z-Argüelles, Alejandro

doi:10.1080/10826060701199072

Cited by 3 publications

(4 citation statements)

References 22 publications

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“…Fuel cell vehicles are considered of utmost importance in the development of new green hydrogen electric energy vehicles, regarded as one of the ultimate solutions for actual transportations due to its advantages such as fast filling speed, high efficiency, low noise, and zero-emission collective transport [39]. Polymer electrolyte membrane fuel cells (PEMFCs) have attracted attention owing to their advantages for automotive propulsion in electric novel vehicular transport and their remarkable technical progress in the last decade [40][41][42][43][44][45].…”

Section: Hydrogen and Oxygen In A Vehicular Transport Prototypementioning

confidence: 99%

Green Hydrogen, PEM Fuel Cells, and Li-ion Batteries in a Vehicular-Transport Prototype

Solorza-Feria

2023

J. Mex. Chem. Soc.

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Abstract. In this communication, recent advances reported in the literature for the development of water splitting in anionic alkaline and polymeric electrolyzers are provided, with particular focus on the feasibility of application of the green hydrogen obtained from renewables as energy carrier to decarbonize the industry and the transportation sector. Despite green hydrogen technology has been proven to be economically viable, financially attractive, and socially beneficial, result of paramount important to reduce costs and accelerate development of the hydrogen and fuel cells energy technologies for a continuous innovation. The application of the green hydrogen and oxygen from air to Cinvestav hybrid vehicular transport prototype, designed, manufactured, and tested in my electrochemical group of research are presented. Resumen. En esta comunicación, se proporcionan los avances recientes reportados en la literatura para el desarrollo de la electrolisis del agua en electrolizadores poliméricos y alcalinos, con especial atención a la viabilidad de la aplicación del hidrógeno verde, obtenido de las energías renovables como vector de energía para descarbonizar la industria y el sector transporte. A pesar de que se ha demostrado que la tecnología del hidrógeno verde es económicamente viable, financieramente atractivo y socialmente beneficioso, resulta de suma importancia reducir costos y acelerar el desarrollo de las tecnologías energéticas de hidrógeno y celdas de combustible para una innovación continua. Se presenta la aplicación del hidrógeno verde y oxígeno del aire al prototipo de transporte vehicular híbrido Cinvestav, diseñado, fabricado y probado en mi grupo de investigación en electroquímica.

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Section: Hydrogen and Oxygen In A Vehicular Transport Prototypementioning

confidence: 99%

Green Hydrogen, PEM Fuel Cells, and Li-ion Batteries in a Vehicular-Transport Prototype

Solorza-Feria

2023

J. Mex. Chem. Soc.

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“…Hence, the possibility that the endothelium has specific receptors for papC should not be ruled out [30]. The pap C in vascular endothelium as well as in platelets has shown dose-dependent effects, suggesting that specific receptors for papC could be present in different cells [2].…”

Section: Discussionmentioning

confidence: 99%

“…Thrombin activates APC by cleavage between arginine-169 www.fhc.viamedica.pl and serine-170 with the subsequent release of the protein C activation peptide (papC), a 12-amino acid peptide. The papC inhibits platelet aggregation in the presence of epinephrine and thrombin [2]. APC possesses antihemostatic and antithrombotic effects and participates in inflammation and apoptotic processes [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Protein C activation peptide inhibits the expression of ICAM-1, VCAM-1, and interleukin-8 induced by TNF-a in human dermal microvascular endothelial cells

Pina-Canseco¹,

Páez²,

Massó³

et al. 2012

Folia Histochem Cytobiol.

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Activated protein C (APC) is generated from the cleavage of protein C by thrombin coupled to thrombomodulin and, subsequently, is released as protein C activation peptide (papC). The aim of this study was to evaluate the effect of papC on human dermal microvascular endothelial cells (HMEC-1), activated with 5 ng/ /mL TNF-a. Flow cytometry showed that papC inhibited the expression of VCAM-1 and ICAM-1, after activation with TNF-a. Similarly, RT-PCR analysis revealed that 2 and 4 pM papC inhibited the expression of VCAM-1 and IL-8 mRNA in TNF-a-treated HMEC-1. In addition, the expression of endothelial nitric oxide synthase (eNOS) increased in HMEC-1 treated with papC, compared to those without treatment. Furthermore, Jurkat cell adhesion to HMEC-1 induced by TNF-a was significantly inhibited after the addition of papC, compared to HMEC-1 without papC (p = 0.03). Finally, a control peptide analog to papC showed no effect on the expression of ICAM and VCAM on the surface of HMEC-1. In conclusion, our results suggest that papC exerts antiinflammatory effects on endothelial cells.

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“…Thrombin activates APC by cleavage between arginine-169 and serine-170 with the subsequent release of the protein C activation peptide (papC), a 12-amino acid peptide. The papC inhibits platelet aggregation in the presence of epinephrine and thrombin [2]. APC possesses antihemostatic and antithrombotic effects and participates in inflammation and apoptotic processes [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Protein C activation peptide inhibits the expression of ICAM-1, VCAM-1, and interleukin-8 induced by TNF-a in human dermal microvascular endothelial cells

Pina-Canseco¹,

Páez²,

Massó³

et al. 2012

Folia Histochem Cytobiol.

Self Cite

View full text Add to dashboard Cite

Activated protein C (APC) is generated from the cleavage of protein C by thrombin coupled to thrombomodulin and, subsequently, is released as protein C activation peptide (papC). The aim of this study was to evaluate the effect of papC on human dermal microvascular endothelial cells (HMEC-1), activated with 5 ng//mL TNF-α. Flow cytometry showed that papC inhibited the expression of VCAM-1 and ICAM-1, after activation with TNF-a. Similarly, RT-PCR analysis revealed that 2 and 4 pM papC inhibited the expression of VCAM-1 and IL-8 mRNA in TNF-α-treated HMEC-1. In addition, the expression of endothelial nitric oxide synthase(eNOS) increased in HMEC-1 treated with papC, compared to those without treatment. Furthermore, Jurkat cell adhesion to HMEC-1 induced by TNF-a was significantly inhibited after the addition of papC, compared to HMEC-1 without papC (p = 0.03). Finally, a control peptide analog to papC showed no effect on the expression of ICAM and VCAM on the surface of HMEC-1. In conclusion, our results suggest that papC exerts anti-inflammatory effects on endothelial cells.

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Interaction of the Protein C Activation Peptide with Platelets

Cited by 3 publications

References 22 publications

Green Hydrogen, PEM Fuel Cells, and Li-ion Batteries in a Vehicular-Transport Prototype

Green Hydrogen, PEM Fuel Cells, and Li-ion Batteries in a Vehicular-Transport Prototype

Protein C activation peptide inhibits the expression of ICAM-1, VCAM-1, and interleukin-8 induced by TNF-a in human dermal microvascular endothelial cells

Protein C activation peptide inhibits the expression of ICAM-1, VCAM-1, and interleukin-8 induced by TNF-a in human dermal microvascular endothelial cells

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