Interaction of tocopherols and phenolic compounds with membrane lipid components: Evaluation of their antioxidant activity in a liposomal model system

Gutiérrez, Mario; Díaz, Antonio; Madariaga, M. Africa de; Sagristá, Maria Lluïsa; Casado, F. Javier; Mora, Miguel A.

doi:10.1016/s0024-3205(03)00120-6

Cited by 59 publications

(46 citation statements)

References 48 publications

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“…The present study showed a decrease in liver glycogen which may be due to excessive adrenergic activity, as suggested by several authors (Müller et al, 1999), although there are reports concerning other properties of R. offi cinalis that may contribute to the preventive and regenerative effect found in our studies (Fahim et al, 1999;Zeng et al, 2001;Sotelo et al, 2002;Gutiérrez et al, 2003;Yahuaca-Mendoza et al, 2005).…”

Section: Discussionsupporting

confidence: 80%

Oxidative stress modulation by Rosmarinus officinalis in CCl₄‐induced liver cirrhosis

Gutiérrez

Alvarado

Presno

et al. 2009

Phytotherapy Research

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Rosmarinus offi cinalis (Lamiaceae) possesses antioxidant activity and hepatoprotective effects, and so may provide a possible therapeutic alternative for chronic liver disease. The effect produced by a methanolic extract of Rosmarinus offi cinalis on CCl 4 -induced liver cirrhosis in rats was investigated using both prevention and reversion models. Over the course of the development of cirrhosis, the increased enzymatic activities of gammaglutamyl transpeptidase and alanine aminotransferase, and the rise in bilirubin levels caused by CCl 4 administration, were prevented by Rosmarinus offi cinalis co-administration. When the cirrhosis by oxidative stress was evaluated as an increase on liver lipoperoxidation, total lipid peroxides, nitric oxide in serum, and loss of erythrocyte plasma membrane stability, R. offi cinalis was shown to prevent such alterations. On cirrhotic animals treated with CCl 4 , histological studies showed massive necrosis, periportal infl ammation and fi brosis which were modifi ed by R. offi cinalis. These benefi ts on experimental cirrhosis suggest a potential therapeutic use for R. offi cinalis as an alternative for liver cirrhosis.

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Section: Discussionsupporting

confidence: 80%

Oxidative stress modulation by Rosmarinus officinalis in CCl₄‐induced liver cirrhosis

Gutiérrez

Alvarado

Presno

et al. 2009

Phytotherapy Research

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“…There are reports showing that the presence of tocopherol in a suspension of cells promotes fusion [4,5]. In model studies with DPPC and DPMC membranes, it has been shown that the presence of tocopherol in the membrane stabilizes its structure [6,7] via the formation of complexes with membrane lipids [8][9][10]. The tocopherol concentration in the above-described experiments was usually between 2 and 8 mol%, and never higher than 12 mol%.…”

Section: Introductionmentioning

confidence: 93%

Spectroscopic studies of D-α-tocopherol concentration-induced transformation in egg phosphatidylcholne vesicles

Dwiecki¹,

Górnaś²,

Wilk

et al. 2007

Cellular and Molecular Biology Letters

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Abstract:The effects of embedding up to 60 mol% of α-tocopherol (α-Toc) on the morphology and structure of the egg phosphatidylcholine (PC) membrane were studied using spectroscopic techniques. The resulting vesicles were subjected to turbidometric and dynamic light scattering measurements to evaluate their size distribution. The α-Toc intrinsic fluorescence and its quenching was used to estimate the tocopherol position in the membrane. Optical microscopy was used to visualize morphological changes in the vesicles during the inclusion of tocopherol into the 2 mg/ml PC membrane. The incorporation of up to 15 mol% of tocopherol molecules into PC vesicles is accompanied by a linear increase in the fluorescence intensity and the simultaneous formation of larger, multilamellar vesicles. Increasing the tocopherol concentration above 20 mol% induced structural and morphological changes leading to the disappearance of micrometer-sized vesicles and the formation of small unilamellar vesicles of size ranging from 30 to 120 nm, mixed micelles and non-lamellar structures.

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“…With respect to reference antioxidants, there have been conflicting reports concerning membrane fluidity modification. Alpha-tocopherol and quercetin induced membrane rigidification in studies of Yoshida et al 38 and Tsuchiya et al, 39 whereas these compounds induced membrane fluidization in studies of Gutiérrez et al 8 and Saija et al 40 Antioxidants show inhibitory effects on lipid peroxidation by rigidifying lipid bilayers to hinder the diffusion of free radicals in membrane lipid environments. 41 They also scavenge free radicals, which is more efficient in fluidized (disordered) lipid bialyers.…”

Section: Speculative Antioxidant Mechanism For Anestheticsmentioning

confidence: 99%

“…In previous fluorometric analyses, fluorescent probes such as 1-palmitoyl-2-parinoylphosphatidylcholine, 21 3-(p-(6-phenyl)-1,3,5-hexatrienyl)phenylpropionic acid, 8,22 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid, 23 and fluorescein 9 were incorporated into liposomal membranes. All of them are methodologically based on the measurement of quenching fluorescent signals resulting from the direct reaction of the probes themselves with oxidants.…”

Section: System Validationmentioning

confidence: 99%

“…7 Liposomes are one of the useful analytical tools for studying the lipid peroxidation in biological model membranes. [8][9][10] They enable us to examine the function of specific lipids, to manipulate the analytical conditions on membrane composition and to avoid the interference from cell components. Liposomal membranes also have the desirable property to minimize the decomposition of hydroperoxides in lipid bilayers.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Activity Analysis by Liposomal Membrane System and Application to Anesthetics

et al. 2008

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Antioxidant activity was analyzed using the liposomal membrane system for lipid peroxidation. Diphenyl-1-pyrenylphosphine incorporated into lipid-bilayer liposomes, which were prepared with unsaturated phospholipids and cholesterol, was converted to the corresponding fluorescent phosphine oxide by the reaction with lipid hydroperoxides. Under the optimal conditions, the liposomes were pretreated with tested compounds. Thereafter, they were reacted with oxidants: 1 -50 mM peroxynitrite, 1.0 mM hydrogen peroxide or 0.4 mM t-butylhydroperoxide, followed by fluorometric analysis. The antioxidant activity was determined by comparing the obtained fluorescence intensities with controls. The proposed method was successfully applicable to various anesthetics (10 and 100 mM for each) and reference antioxidants: a-tocopherol (1.0 and 2.5 mM), quercetin (1.0 and 5.0 mM) and (-)-epigallocatechin-3-gallate (1.0 and 5.0 mM) with intraand inter-assay C.V.s of 0.2 -7.0%. In analyses of general and local anesthetics, propofol was the most active, and showed a concentration of 6.8 mM to produce 50% inhibition against the peroxynitrite-induced lipid peroxidation of cell model membranes, supporting its potential benefit in clinical use.

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Interaction of tocopherols and phenolic compounds with membrane lipid components: Evaluation of their antioxidant activity in a liposomal model system

Cited by 59 publications

References 48 publications

Oxidative stress modulation by Rosmarinus officinalis in CCl₄‐induced liver cirrhosis

Oxidative stress modulation by Rosmarinus officinalis in CCl₄‐induced liver cirrhosis

Spectroscopic studies of D-α-tocopherol concentration-induced transformation in egg phosphatidylcholne vesicles

Antioxidant Activity Analysis by Liposomal Membrane System and Application to Anesthetics

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Interaction of tocopherols and phenolic compounds with membrane lipid components: Evaluation of their antioxidant activity in a liposomal model system

Cited by 59 publications

References 48 publications

Oxidative stress modulation by Rosmarinus officinalis in CCl4‐induced liver cirrhosis

Oxidative stress modulation by Rosmarinus officinalis in CCl4‐induced liver cirrhosis

Spectroscopic studies of D-α-tocopherol concentration-induced transformation in egg phosphatidylcholne vesicles

Antioxidant Activity Analysis by Liposomal Membrane System and Application to Anesthetics

Contact Info

Product

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Oxidative stress modulation by Rosmarinus officinalis in CCl₄‐induced liver cirrhosis

Oxidative stress modulation by Rosmarinus officinalis in CCl₄‐induced liver cirrhosis