Interaction of Wheat Germ Agglutinin with Porphyrin Compounds—Potential Anticancer Agents

Bogoeva, Vanya; Petrova, Lidiya; Ivanov, Ivan B.; Kulina, Hristina; Russev, George

doi:10.5504/bbeq.2011.0057

Cited by 7 publications

(2 citation statements)

References 28 publications

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“…The curve revealed cooperative interactions which indicated that binding of one FeTMPyP molecule facilitated the interaction of the next FeTMPyP molecules by enhancing the affinity of the vacant binding sites on the lectin. The obtained results demonstrated that the legume lectin Con A bound FeTMPyP with high affinity, similar to that of the non-legume lectin WGA for iron porphyrins [ 18 , 19 ].…”

Section: Resultsmentioning

confidence: 83%

New Activity of a Protein from Canavalia ensiformis

2014

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Concanavalin A is a legume lectin which preferentially agglutinates transformed cells and shows antitumor effects on human breast carcinoma cells in vitro and in vivo. It is considered as a new potential antineoplastic agent targeting apoptosis, autophagy, and anti-angiogenesis in preclinical or clinical trials for cancer therapeutics, which has recently become the object of intensive study. In the present investigation, we show the capacity of the lectin to bind manganese, gold, iron, and zinc porphyrins: all potential anticancer agents. The interaction of the legume lectin with the studied compounds has been investigated by tryptophan fluorescence, showing conformational changes within the quaternary and tertiary structures of the protein. The binding of Con A with manganese, gold, and iron porphyrins, as well as adenine, was studied by fluorescence quenching. In contrast, the interaction of Con A with zinc porphyrin caused an increase in Trp fluorescence and a red shift of 10 nm of the emission maximum position.However, the binding of Con A to iron porphyrin was accompanied by a 5 nm blue shift of the emission maximum, and a kD of 0.95 ± 0.13 μM was calculated, respectively. The sigmoidal shape of the curve showed cooperative interactions, which indicated the presence of more than one class of binding site within the Con A molecule for iron porphyrin, confirmed by the Hill slope (h = 1.89±0.46). We have found that the legume lectin interacts with porphyrins and adenine with an affinity (0.14–1.89 µM) similar to that of the non-legume lectin, wheat germ agglutinin. In conclusion, the protein Con A shows new binding activity towards porphyrins with anticancer activities and could find prospective application as a drug delivery molecule that specifically targets cancer cells.

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Section: Resultsmentioning

confidence: 83%

New Activity of a Protein from Canavalia ensiformis

2014

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“…Recently, T/Tn specific lectins have been identified as potential targeting molecules [29]. The non-covalent binding of porphyrins to lectins was well studied [30, Bogoeva, 2010 #314], particularly the interaction of wheat germ agglutinin (WGA) [31][32][33][34] and Con A with porphyrins was evaluated and affinity constants in micromolar range were calculated, accordingly [35,36].…”

Section: Why Lectins Are Useful Tools For the Targeted Therapy?mentioning

confidence: 99%

Dual function of lectins — new perspectives in targeted photodynamic therapy

Bogoeva

Petrova

Yordanova

et al. 2019

J. Porphyrins Phthalocyanines

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Porphyrins and phthalocyanines are photosensitizers (PS) that are used in clinical imaging, detection of cancer cells and are particularly applied in photodynamic therapy (PDT). Many scientists have been focused on the design of different porphyrin compounds. However, similar to other anti-cancer agents, they cannot selectively recognize tumor tissues. Scientists are seeking new methods to overcome this problem and to find appropriate targeted delivery strategies. Plant lectins are especially suitable molecules for such targeting as they preferentially recognize specific antigens on the glycosylated cancer cells. This review will give more detailed information about the dual function of lectins and their interactions with PSs, which is a new perspective in targeted PDT. The implications and potential applications of such studies will also be discussed.

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