Interaction of yttrium compounds with serum and serum constituents in vitro

Rosoff, Betty; Lewin, Ruth; Hart, Hiram E.; Williams, Grady L.; László, Dániel

doi:10.1016/0003-9861(58)90308-4

Cited by 19 publications

(7 citation statements)

References 11 publications

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“…The maximum measured extent, aM, and number, r, of Y3+ ions (in 20mM-yttrium chloride) bound to the three proteins has been estimated by method A (Table 3). The resulting value for bovine serum albumin is of the same order as that obtained by Rosoff et al (1958), who found that n= 6 at pH5'3 by equilibrium dialysis.…”

supporting

confidence: 82%

“…The advantages of this method of measuring the binding are (i) that much smaller quantities of material than would be required by most other methods can be used (cf. Rosoff, Lewin, Hart, Williams & Lazlo, 1958) and (ii) that the type of interaction determined by displacement of the titration curves is a strong one, since competition with H+ for carboxylate anions is occurring. The special disadvantage of the method is that it reveals only the number of such titratable groups involved: the actual number of metal ions bound to them can only be ascertained by making certain further assumptions, which have been later justified by their self-consistency.…”

mentioning

confidence: 99%

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The binding of calcium and yttrium ions to a glycoprotein from bovine cortical bone

Williams

Peacocke

1967

Biochemical Journal

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The binding of Ca2+ and Y3+ to an acidic glycoprotein from bovine cortical bone, bone sialoprotein, was determined from the titration curves at I 0 2 in the presence and absence of the cations. The binding of Y3+ was greater than that of Ca2+. The value for the association constant, k, for the interaction with Y3+ increased with pH, from logik 2-93 at pH 3-4 to logk 3 50 at pH4-4, and the number of binding sites/mol. increased from 4 6 at pH3 4 to 9-1 at pH4 4. It is proposed that the binding site consists of three carboxyl groups, but it is likely that the binding is a strong electrostatic interaction rather than a co-ordination linkage. A chondroitin sulphateprotein complex also extracted from bovine cortical bone interacted with Y3+ and Ca2+ to a similar extent as did bone sialoprotein. It is suggested that these materials are present in bone at the resting and resorbing surfaces and that they contribute to the deposition of yttrium, americium and plutonium at these sites.

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supporting

confidence: 82%

mentioning

confidence: 99%

The binding of calcium and yttrium ions to a glycoprotein from bovine cortical bone

Williams

Peacocke

1967

Biochemical Journal

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“…(23) Thus one would expect a relationship between chelate strength and the passage of ld4Ce chelates across the placenta and their subsequent deposition in the fetus. (s) The strength of the goY-chelate bond also determined the amount of that passed through membrane ultrafilters.…”

Section: Table 2 Amount Of1mce Deposited In One Rat Fetus After Intrmentioning

confidence: 99%

The Fetal Uptake of Cerium-144-praseodymium-144 after Injection of Its Chelates to Pregnant Rat

Beňo¹

1973

Health Physics

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On the 17th day of pregnancy albino rats were injectedintravenously with 144Ce-144Pr* chloride, citrate, nitrilotriacetate (NTA), or ethylenediaminetetraacetate (EDTA) and sacrificed at intervals up to 4 days later. The radioactivity of fetuses, placentae, amniotic fluid, maternal blood and maternal femora was measured. Deposition of the four '@Ce compounds in the fetuses was small: the greatest deposition, 0.013 %of the injected dose deposited in one fetus, was observed 4 days after injection of lMCeEDTA. The radioactivity of the fetuses generally increased with time after injection 0f'~~CeC1, or lMCeEDTA. Deposition of144Ce in the fetuses was greatest after injection ofl'CeEDTA or 'We-citrate and least after injection of 144CeC1, or 'UCeNTA, but the differences were not always significant. Deposition of lUCe in the placenta was greatest after injection of lQ4CeEDTA. DeDosition of lMCe in maternal bone was significantly greater after injection of 'ace chelates than after injection of lWeC1,.

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“…In vitro studies have shown a correlation between the stability constant of a metal chelate and its filterability: the ultrafiltration of strong metal chelates from serum indicated preferential binding of the metal to the chelate as opposed to 6 177 binding of the metal by body competitors. (5)(6)(7) In the search for agents which may be effective in removing yttrium from the body, chelating agents of the polyamino acid type have been selected for study since they have a strong affinity €or yttrium and since the urinary excretion of these chelating agents was found to be high.…”

Section: Introductionmentioning

confidence: 98%

“…(5)(6)(7) In the search for agents which may be effective in removing yttrium from the body, chelating agents of the polyamino acid type have been selected for study since they have a strong affinity €or yttrium and since the urinary excretion of these chelating agents was found to be high. When diethylenetriaminepenta-acetic acid (DTPA) was injected intravenously as the yttrium chelate, labeled with Y S 0 , the urinary excretion of YS0 was found to be almost 100 per cent in 24 hr.…”

Section: Introductionmentioning

confidence: 99%

Effect of Chelating Agents on the Removal of Yttrium and Lanthanum from Man

Rosoff¹,

Ritter²,

Sullivan³

et al. 1961

Health Physics

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Yttrium chelate excretion studies have been conducted in this laboratory stimulated by the interest in the removal of fission products from man. Since diethylenetriaminepentaacetic acid (DTPA) has a high affinity for yttrium and is almost quantitatively excreted in 24 hr, the effectiveness of DTPA in removing yttrium from the body was studied in eight paticnts. Yttrium was injected intravenously as the nitrilotriacetate (YQo-NTA), a weak chelate, and 24 hr later, 500 mg DTPA was infused daily on four successive days. The YgO excretion was then compared with the results obtained with equivalent amounts of EDTA.The effectiveness of 2 g of either chelate was also studied. The infusions of 500 mg DL'PA increased the yttrium excretion from four to eight times over the base line excretion. DTPA was more effective than EDTA and a fourfold increase of either chelate did not result in an appreciably higher excretion of the metal chelate than was achieved with the 500 mg dose. In studies carried out in two patients, doses of 100 mg DTPA were found to be less effective than 500 mg of this chelating agent. Similar studies of the removal of La140 indicate that DTPA is also more effective than EDTA in removing this rare earth isotope from man. The significance of these decontamination studies and their possible application for fission product -removal will be discussed.

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Interaction of yttrium compounds with serum and serum constituents in vitro

Cited by 19 publications

References 11 publications

The binding of calcium and yttrium ions to a glycoprotein from bovine cortical bone

The binding of calcium and yttrium ions to a glycoprotein from bovine cortical bone

The Fetal Uptake of Cerium-144-praseodymium-144 after Injection of Its Chelates to Pregnant Rat

Effect of Chelating Agents on the Removal of Yttrium and Lanthanum from Man

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