Interactions among chemical components of Cocoa tea (Camellia ptilophylla Chang), a naturally low caffeine-containing tea species

Abstract

In the 1980s, a novel tea species, Cocoa tea (Camellia ptilophylla Chang), was discovered in Southern China with surprisingly low caffeine content (0.2% by dry weight). Although its health promoting characteristics have been known for a while, a very limited amount of scientific research has been focused on Cocoa tea. Herein, a systematic study on Cocoa tea and its chemical components, interactions and bioactivities was performed. YD tea (Yunnan Daye tea, Camellia sinensis), a tea species with a high caffeine content (5.8% by dry weight), was used as a control. By UV-Vis spectrometry, High Performance Liquid Chromatography (HPLC), and Flame Atomic Absorption Spectrometry (FAAS) for chemical composition analysis, C-2 epimeric isomers of tea catechins and theobromine were found to be the major catechins and methylxanthine in Cocoa tea, respectively. More gallated catechins, methylxanthines, and proteins were detected in Cocoa tea compared with YD tea. Moreover, the tendency of major components in Cocoa tea for precipitation was significantly higher than that in YD tea. Catechins, methylxanthines, proteins, iron, calcium, and copper were presumed to be the origins of molecular interactions in Cocoa tea and YD tea. The interactions between catechins and methylxanthines were highly related to the galloyl moiety in catechins and methyl groups in methylxanthines. In vitro anti-inflammatory activity assays revealed that Cocoa tea was a more potent inhibitor of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated macrophage cells (RAW 264.7) than YD tea. This study constructs a solid phytochemical foundation for further research on the mechanisms of molecular interactions and the integrated functions of Cocoa tea.

The Effect of Ursolic Acid on Leishmania (Leishmania) amazonensis Is Related to Programed Cell Death and Presents Therapeutic Potential in Experimental Cutaneous Leishmaniasis

“…amazonensis infection [ 54 , 55 , 56 ]. In addition, NO can be triggered by different stimuli as well as natural compounds from plants [ 57 ]. In the present study, infected peritoneal macrophages treated with UA produced elevated amounts of NO at 24 and 72h compared to controls.…”

Removal of imidacloprid and acetamiprid from tea infusions by microfiltration membrane

“…GA, eight catechins (EGCG, GCG, ECG, EGC, CG, GC, EC and C), and three methylxanthines (CAF, theobromine and theophylline) were determined by HPLC as described by Lin et al . (). The free amino acids of tea infusions before and after microfiltration were determined on UV‐Vis spectrometer according to the Chinese National Standard GB/T 8314‐2013.…”

Phytochemicals, Anti‐Inflammatory, Antiproliferative, and Methylglyoxal Trapping Properties of Zijuan Tea

“…Total anthocyanins content was measured with the pH differential method, expressed as cyanidin 3‐ O ‐glucoside equivalents (Lee and others ). As described in our recent study (Lin and others ), total phenolics content was determined according to the Folin‐Ciocalteu assay, expressed as gallic acid equivalents. Monomeric catechins and caffeine of Zijuan tea extracts (1 mg/mL) were analyzed using an Agilent 1200 series HPLC system (Agilent Technologies, Inc., Santa Clara, Calif., U.S.A.).…”