Interactions among enzymes of the
            <i>Arabidopsis</i>
            flavonoid biosynthetic pathway

Burbulis, Ian; Winkel-Shirley, Brenda

doi:10.1073/pnas.96.22.12929

Cited by 338 publications

(225 citation statements)

References 50 publications

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“…Overall, these data indicate that the expression of structural genes is regulated during fruit ripening, so that many of them are more expressed at the MG or TU stages in both tissues (Figures 3B and 4B) and that the higher early activation of structural genes at these stages may be important to prepare the multienzymatic complexes in which the flavonoid biosynthetic enzymes are probably organized (42,43), in order to activate the synthesis of intermediate metabolites and final products during the TU stage.…”

Section: Effect Of Uv-b Depletion On Phenolic Biosynthesis In Peelmentioning

confidence: 96%

Flavonoid Profiling and Biosynthetic Gene Expression in Flesh and Peel of Two Tomato Genotypes Grown under UV-B-Depleted Conditions during Ripening

Giuntini

Lazzeri

Calvenzani

et al. 2008

J. Agric. Food Chem.

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The effect of shielding solar ultraviolet B radiation on the accumulation of some flavonoids and their precursor hydroxycinnamic acids in tomato (Solanum lycopersicum) was evaluated by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). In particular, flesh and peel of two tomato hybrids, DRW 5981 and Esperanza, were separately analyzed. The hybrids have been chosen for their different responses to the light, since it was previously reported that they show different pigmentation and opposite behavior under UV-B in terms of carotenoids and ascorbic acid content at different ripening stages. To determine the effect of UV-B radiation during tomato ripening, we also measured the expression of some flavonoid biosynthetic genes by realtime reverse transcription-polymerase chain reaction (RT-PCR) analysis. The results allowed us to conclude that UV-B radiation deeply and differentially affects the content of the considered flavonoids and hydroxycinnamic acids as well as the expression of some of their biosynthetic genes in both flesh and peel during the ripening process. On the other hand, the collected data clearly showed that this influence varies between different genotypes. We conclude that the use of specific plastic covers able to eliminate UV-B radiation may be an environmentally friendly approach to modulate the expression of structural genes and, in turn, to enhance healthy antioxidant compounds in fruits of specific tomato cultivars.

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Section: Effect Of Uv-b Depletion On Phenolic Biosynthesis In Peelmentioning

confidence: 96%

Flavonoid Profiling and Biosynthetic Gene Expression in Flesh and Peel of Two Tomato Genotypes Grown under UV-B-Depleted Conditions during Ripening

Giuntini

Lazzeri

Calvenzani

et al. 2008

J. Agric. Food Chem.

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“…This includes metabolic systems in which catalytic efficiency and control of end-product specificity can be enhanced by the assembly of enzymes into macromolecular complexes (Burbulis and Winkel-Shirley, 1999). Therefore, it is possible that spermidine formed by the SPDS-catalyzed reaction may be channeled effectively to SPMS, modulating the formation of the end product of the pathway.…”

Section: Discussionmentioning

confidence: 99%

A Polyamine Metabolon Involving Aminopropyl Transferase Complexes in Arabidopsis

Panicot

Minguet

Ferrando³

et al. 2002

Plant Cell

165

126

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The conversion of putrescine to spermidine in the biosynthetic pathway of plant polyamines is catalyzed by two closely related spermidine synthases, SPDS1 and SPDS2, in Arabidopsis. In the yeast two-hybrid system, SPDS2 was found to interact with SPDS1 and a novel protein, SPMS (spermine synthase), which is homologous with SPDS2 and SPDS1. SPMS interacts with both SPDS1 and SPDS2 in yeast and in vitro. Unlike SPDS1 and SPDS2, SPMS failed to suppress the spe ⌬ 3 deficiency of spermidine synthase in yeast. However, SPMS was able to complement the spe ⌬ 4 spermine deficiency in yeast, indicating that SPMS is a novel spermine synthase. The SPDS and SPMS proteins showed no homodimerization but formed heterodimers in vitro. Pairwise coexpression of hemagglutinin-and c-Myc epitope-labeled proteins in Arabidopsis cells confirmed the existence of coimmunoprecipitating SPDS1-SPDS2 and SDPS2-SPMS heterodimers in vivo. The epitope-labeled SPDS and SPMS proteins copurified with protein complexes ranging in size from 650 to 750 kD. Our data demonstrate the existence of a metabolon involving at least the last two steps of polyamine biosynthesis in Arabidopsis.

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“…Other data from experiments employing classical approaches such as double radioactive labeling assays (13)(14)(15), enzyme co-purification (15,16), and immunohistochemical localization (17) support the presence of MECs among enzymes in the entry point or downstream pathways. Investigation of possible MECs in phenylpropanoid metabolism has been further pursued in recent years using various molecular techniques, revealing direct proteinprotein interactions among enzymes in the flavonoid pathway in Arabidopsis (18) and the elicitor-dependent endoplasmic reticulum (ER)-localization of an otherwise cytosolic isoflavone Omethyltransferase in alfalfa (19). However, there is still no direct evidence for PAL-C4H interaction to form an MEC.…”

mentioning

confidence: 99%

Reconstitution of the Entry Point of Plant Phenylpropanoid Metabolism in Yeast (Saccharomyces cerevisiae)

Douglas

2004

Journal of Biological Chemistry

120

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Interactions among enzymes of the Arabidopsis flavonoid biosynthetic pathway

Cited by 338 publications

References 50 publications

Flavonoid Profiling and Biosynthetic Gene Expression in Flesh and Peel of Two Tomato Genotypes Grown under UV-B-Depleted Conditions during Ripening

Flavonoid Profiling and Biosynthetic Gene Expression in Flesh and Peel of Two Tomato Genotypes Grown under UV-B-Depleted Conditions during Ripening

A Polyamine Metabolon Involving Aminopropyl Transferase Complexes in Arabidopsis

Reconstitution of the Entry Point of Plant Phenylpropanoid Metabolism in Yeast (Saccharomyces cerevisiae)

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