Interactions Between Copper Homeostasis and Metabolism in Plants

Schulten, Anna; Krämer, Ute

doi:10.1007/124_2017_7

Cited by 19 publications

(13 citation statements)

References 174 publications

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“…We confirmed by RT-PCR that SPL1 and SPL12 were expressed in 21-day old WT seedlings under both Cu-and Zn-deficient growth conditions ( Fig. S1), which we generated by removing trace metal contaminations arising from agar and glassware and by omitting Cu and Zn, respectively, from the synthetic growth media (Schulten and Krämer 2018). In addition, we confirmed that full-length SPL1 and SPL12 transcripts were absent or reduced to nearly undetectable levels under all our growth conditions in the mutants spl1 spl12 and spl1 spl7 spl12 (Fig.…”

Section: Resultssupporting

confidence: 58%

Do Arabidopsis Squamosa promoter binding Protein-Like genes act together in plant acclimation to copper or zinc deficiency?

Schulten

Bytomski

Quintana

et al. 2019

Preprint

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The genome of Arabidopsis thaliana encodes approximately 260 copper (Cu)-dependent proteins, which include enzymes in central pathways of photosynthesis, respiration and responses to environmental stress. Under Cu-deficient growth conditions, Squamosa promoter binding Protein-Like 7 (SPL7) activates the transcription of genes encoding Cu acquisition systems and mediates a metabolic reorganization to economize on Cu. The transcription factor SPL7 groups among comparably large proteins in the SPL family, which additionally comprises a second group of small SPL proteins targeted by miRNA156 with roles in plant development. SPL7 shares extended regions of sequence homology with SPL1 and SPL12. Therefore, we investigated the possibility of a functional overlap between these three members of the group of large SPL family proteins. We compared the spl1 spl12 double mutant and the spl1 spl7 spl12 triple mutant with the wild type and the spl7 single mutant under normal and Cu-deficient growth conditions. Biomass production, chlorophyll content and tissue elemental composition at the seedling stage, as well as plant and flower morphology during reproductive stages, confirmed the involvement of SPL7, but provided no indication for important roles of SPL1 or SPL12, in the acclimation of Arabidopsis to Cu deficiency. Furthermore, we analyzed the effects of zinc (Zn) deficiency on the same set of mutants. Different from what is known in the green alga Chlamydomonas reinhardtii, Arabidopsis did not activate Cu deficiency responses under Zn deficiency, and there was no Cu overaccumulation in either shoot or root tissues of Zn-deficient wild-type plants. Known Zn deficiency responses were unaltered in spl7, spl1 spl12 and spl1 spl7 spl12 mutants. We observed that CuZnSOD activity is strongly downregulated in Zn-deficient A. thaliana, in association with an about 94% reduction in the abundance of the CDS2 transcript, a known target of miR398. However, different from the known Cu deficiency responses of Arabidopsis, this Zn deficiency response was independent of SPL7 and not associated with an upregulation of MIR398b primary transcript levels. Our data suggest that there is no conservation in A. thaliana of the crosstalk between Zn and Cu homeostasis mediated by the single SPL family protein CRR1 in Chlamydomonas. In the future, resolving how the specificity of SPL protein activation and recognition of target gene promoters is achieved will advance our understanding of the specific functions of different SPL family proteins in either Cu deficiency response regulation or growth and development of land plants.

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Section: Resultssupporting

confidence: 58%

Do Arabidopsis Squamosa promoter binding Protein-Like genes act together in plant acclimation to copper or zinc deficiency?

Schulten

Bytomski

Quintana

et al. 2019

Preprint

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“…In order to address the possibility of a functional overlap between paralogous members of the SPL protein family, we compared Cu and Zn deficiency responses of the spl1 spl12 double and the spl1 spl7 spl12 triple mutant to the wild type (WT) and the spl7 single mutant. We confirmed by RT‐PCR that SPL1 and SPL12 were expressed in 21‐day‐old WT seedlings under both Cu‐ and Zn‐deficient growth conditions (Figure ), which we generated by removing trace metal contaminations arising from agar and glassware and by omitting Cu and Zn, respectively, from the synthetic growth media (Schulten & Krämer, ). In addition, we confirmed that full‐length SPL1 and SPL12 transcripts were absent or reduced to nearly undetectable levels under all our growth conditions in the mutants spl1 spl12 and spl1 spl7 spl12 (Figure ).…”

Section: Resultssupporting

confidence: 54%

“…We confirmed by RT-PCR that SPL1 and SPL12 were expressed in 21-day-old WT seedlings under both Cu-and Zn-deficient growth conditions ( Figure S1), which we generated by removing trace metal contaminations arising from agar and glassware and by omitting Cu and Zn, respectively, from the synthetic growth media (Schulten & Krämer, 2018). In addition, we confirmed that full-length SPL1 and SPL12 transcripts were absent or reduced to nearly undetectable levels under all our growth conditions in the mutants spl1 spl12 and spl1 spl7 spl12 ( Figure S1).…”

Section: Re Sultssupporting

confidence: 59%

Do ArabidopsisSquamosa promoter binding Protein‐Likegenes act together in plant acclimation to copper or zinc deficiency?

et al. 2019

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The genome of Arabidopsis thaliana encodes approximately 260 copper (Cu)‐dependent proteins, which includes enzymes in central pathways of photosynthesis, respiration and responses to environmental stress. Under Cu‐deficient growth conditions, Squamosa promoter binding Protein‐Like 7 ( SPL 7) activates the transcription of genes encoding Cu acquisition systems, and it mediates a metabolic reorganization to economize on Cu. The transcription factor SPL 7 groups among comparably large proteins in the SPL family, which additionally comprises a second group of small SPL proteins targeted by mi RNA 156 with roles in plant development. SPL 7 shares extended regions of sequence homology with SPL 1 and SPL 12. Therefore, we investigated the possibility of a functional overlap between these three members of the group of large SPL family proteins. We compared the spl1 spl12 double mutant and the spl1 spl7 spl12 triple mutant with both the wild type and the spl7 single mutant under normal and Cu‐deficient growth conditions. Biomass production, chlorophyll content and tissue elemental composition at the seedling stage, as well as plant and flower morphology during reproductive stages, confirmed the involvement of SPL 7, but provided no indication for important roles of SPL 1 or SPL 12 in the acclimation of Arabidopsis to Cu deficiency. Furthermore, we analyzed the effects of zinc (Zn) deficiency on the same set of mutants. Different from what is known in the green alga Chlamydomonas reinhardtii , Arabidopsis did not activate Cu deficiency responses under Zn deficiency, and there was no Cu overaccumulation in either shoot or root tissues of Zn‐deficient wild type plants. Known Zn deficiency responses were unaltered in spl7 , spl1 spl12 and spl1 spl7 spl12 mutants. We observed that CuZn SOD activity is strongly downregulated in Zn‐deficient A. thaliana , in association with an about 94% reduction in the abundance of the CSD 2 transcript, a known target of miR398. However, different from the known Cu deficiency responses of Arabidopsis, this Zn deficiency response was independent of SPL 7 and not associated with an upregulation of MIR 398b primary transcript levels. Our data suggest that there is no conservation in A. thaliana of the crosstalk between Zn and Cu homeostasis mediated by the single SPL family protein CRR 1 of Chl...

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“…Transcript levels of MYB10 and CYP71B5 are known to respond strongly to Fe deficiency (Buckhout et al, 2009; Yang et al, 2010). BGLU45 and LAC7 are thought to function in cell wall lignification (Chapelle et al, 2012; Schulten and Krämer, 2017).…”

Section: Resultsmentioning

confidence: 99%

Root-to-shoot iron partitioning in Arabidopsis requires IRON-REGULATED TRANSPORTER1 (IRT1)

Quintana

Scholle

et al. 2021

Preprint

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IRON-REGULATED TRANSPORTER1 (IRT1) is the root high-affinity ferrous iron uptake system and indispensable for the completion of the life cycle of Arabidopsis thaliana without vigorous iron (Fe) supplementation. Here we provide evidence supporting a second role of IRT1 in root-to-shoot mobilization of Fe. We show that the irt1-2 (pam42) mutant over-accumulates Fe in roots, most prominently in the cortex of the differentiation zone, when compared to the wild type. Shoots of irt1-2 are severely Fe-deficient according to Fe content and marker transcripts, as expected. We generated irt1-2 lines producing IRT1 mutant variants carrying single amino-acid substitutions of key residues in transmembrane helices IV and V, Ser206 and His232, which are required for transport activity in yeast. In the transgenic Arabidopsis lines, short-term root Fe uptake rates and secondary substrate Mn accumulation resemble those of irt1-2, suggesting that these plants remain incapable of IRT1-mediated root Fe uptake. Yet, IRT1S206A partially complements rosette dwarfing and leaf chlorosis, as well as root-to-shoot Fe partitioning and gene expression defects of irt1-2, all of which are fully complemented by wild-type IRT1. Taken together, these results suggest a function for IRT1 in root-to-shoot Fe partitioning that does not require Fe transport activity of IRT1. Among the genes of which transcript levels are partially dependent on IRT1, we identify MYB DOMAIN PROTEIN10, MYB DOMAIN PROTEIN72 and NICOTIANAMINE SYNTHASE4 as candidates for effecting IRT1-dependent Fe mobilization in roots. Understanding the biological functions of IRT1 will help to improve iron nutrition and the nutritional quality of agricultural crops.

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Interactions Between Copper Homeostasis and Metabolism in Plants

Cited by 19 publications

References 174 publications

Do Arabidopsis Squamosa promoter binding Protein-Like genes act together in plant acclimation to copper or zinc deficiency?

Do Arabidopsis Squamosa promoter binding Protein-Like genes act together in plant acclimation to copper or zinc deficiency?

Do ArabidopsisSquamosa promoter binding Protein‐Likegenes act together in plant acclimation to copper or zinc deficiency?

Root-to-shoot iron partitioning in Arabidopsis requires IRON-REGULATED TRANSPORTER1 (IRT1)

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