Interactions between endocarditis-derived Streptococcus gallolyticus subsp. gallolyticus isolates and human endothelial cells

Vollmer, Tanja; Hinse, Dennis; Kleesiek, Knut; Dreier, Jens

doi:10.1186/1471-2180-10-78

Cited by 44 publications

(62 citation statements)

References 53 publications

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“…In addition, the capacity to adhere to ECM proteins has been suggested to be an important factor in the colonization of the heart valves by oral bacteria (48,53,56). Similar to the case for S. gallolyticus (62), our data revealed that the capacity to invade does not seem to be associated with the ability to form biofilms and that Cnm plays a strain-specific role in biofilm formation. In S. gordonii, inactivation of glucosyltranferase (gtf), which is responsible for biosynthesis of the extracellular polysaccharide glucan that contributes to the adhesion of streptococci to cultured human umbilical vein endothelial cells (59), led to a significant reduction in the ability of the strain to invade these cells.…”

Section: Discussionsupporting

confidence: 62%

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The Collagen-Binding Protein Cnm Is Required for Streptococcus mutans Adherence to and Intracellular Invasion of Human Coronary Artery Endothelial Cells

et al. 2011

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Streptococcus mutans is considered the primary etiologic agent of dental caries, a global health problem that affects 60 to 90% of the population, and a leading causative agent of infective endocarditis. It can be divided into four different serotypes (c, e, f, and k), with serotype c strains being the most common in the oral cavity. In this study, we demonstrate that in addition to OMZ175 and B14, three other strains (NCTC11060, LM7, and OM50E) of the less prevalent serotypes e and f are able to invade primary human coronary artery endothelial cells (HCAEC). Invasive strains were also significantly more virulent than noninvasive strains in the Galleria mellonella (greater wax worm) model of systemic disease. Interestingly, the invasive strains carried an additional gene, cnm, which was previously shown to bind to collagen and laminin in vitro. Inactivation of cnm rendered the organisms unable to invade HCAEC and attenuated their virulence in G. mellonella. Notably, the cnm knockout strains did not adhere to HCAEC as efficiently as the parental strains did, indicating that the loss of the invasion phenotype observed for the mutants was linked to an adhesion defect. Comparisons of the invasive strains and their respective cnm mutants did not support a correlation between biofilm formation and invasion. Thus, Cnm is required for S. mutans invasion of endothelial cells and possibly represents an important virulence factor of S. mutans that may contribute to cardiovascular infections and pathologies.

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Section: Discussionsupporting

confidence: 62%

“…Differences in invasion rates have been shown for other oral bacteria, such as P. gingivalis and S. gordonii (13,23,57), as well as for an inhabitant of the gastrointestinal flora, Streptococcus gallolyticus subsp. gallolyticus (62). Furthermore, certain clinical strains of S. mutans display low expression levels of cnm mRNA (47).…”

Section: Discussionmentioning

confidence: 99%

The Collagen-Binding Protein Cnm Is Required for Streptococcus mutans Adherence to and Intracellular Invasion of Human Coronary Artery Endothelial Cells

et al. 2011

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“…1). Some of the human and animal isolates were kindly provided from the public BCCM/LMG strain collection (http: //bccm.belspo.be/about/lmg.php) (13), whereas the bacteremic isolates were obtained at the Ramón y Cajal University Hospital (1) in Madrid, Spain. All strains were identified to the subspecies level by PCR amplification of an internal fragment of the sodA gene and further nucleotide sequencing (1).…”

Section: Bacterial Isolatesmentioning

confidence: 99%

“…gallolyticus isolates from humans and animals have been described previously (8,10), including the existence of particular virulent clones with increased invasion and adherence abilities, which favor bloodstream infections (11)(12)(13). A recent multilocus sequence typing (MLST)-based study demonstrated a lack of specificity for any particular host or geographical location (10).…”

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Streptococcus gallolyticus subsp. gallolyticus from Human and Animal Origins: Genetic Diversity, Antimicrobial Susceptibility, and Characterization of a Vancomycin-Resistant Calf Isolate Carrying a vanA -Tn 1546 -Like Element

Romero-Hernández¹,

Tedim²,

Sánchez-Herrero³

et al. 2015

Antimicrob Agents Chemother

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The aim of this work was to characterize the antibiotic susceptibility and genetic diversity of 41 Streptococcus gallolyticus subsp. gallolyticus isolates: 18 isolates obtained from animals and 23 human clinical isolates. Antibiotic susceptibility was determined by the semiautomatic Wider system and genetic diversity by pulsed-field gel electrophoresis (PFGE) with SmaI. Animal isolates grouped separately in the PFGE analysis, but no statistical differences in antimicrobial resistance were found between the two groups. The LMG 17956 sequence type 28 (ST28) strain recovered from the feces of a calf exhibited high levels of resistance to vancomycin and teicoplanin (MIC, >256 mg/liter). Its glycopeptide resistance mechanism was characterized by Southern blot hybridization and a primer-walking strategy, and finally its genome, determined by whole-genome sequencing, was compared with four closely related S. gallolyticus subsp. gallolyticus genomes. Hybridization experiments demonstrated that a Tn1546-like element was integrated into the bacterial chromosome. In agreement with this finding, whole-genome sequencing confirmed a partial deletion of the vanY-vanZ region and partial duplication of the vanH gene. The comparative genomic analyses revealed that the LMG 17956 ST28 strain had acquired an unusually high number of transposable elements and had experienced extensive chromosomal rearrangements, as well as gene gain and loss events. In conclusion, S. gallolyticus subsp. gallolyticus isolates from animals seem to belong to lineages separate from those infecting humans. In addition, we report a glycopeptide-resistant isolate from a calf carrying a Tn1546-like element integrated into its chromosome. N ew taxonomic criteria have recently been applied to the Streptococcus bovis/equinus complex, mainly on the basis of the genetic diversity of the sodA gene, which is considered the best target for adequate identification (1). These taxonomic advances have permitted the study of the epidemiological correlations between particular subspecies and specific human pathologies (2), including gastrointestinal colonization by Streptococcus gallolyticus subsp. gallolyticus (formerly Streptococcus bovis biotype I) and its coincidence with colorectal cancer (3).The rates of colonization by S. gallolyticus subsp. gallolyticus are around 5 to 10% in humans but could be much higher in animals (4). Bacteremia and endocarditis are the main relevant clinical manifestations in humans and in avian species (5, 6). The mortality rate in a broiler flock outbreak was 4.3% (7), and the organism has also been implicated in other veterinary pathologies (8, 9).Similarities and differences between S. gallolyticus subsp. gallolyticus isolates from humans and animals have been described previously (8, 10), including the existence of particular virulent clones with increased invasion and adherence abilities, which favor bloodstream infections (11-13). A recent multilocus sequence typing (MLST)-based study demonstrated a lack of specificity for any particu...

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“…Such observations are of interest, as they imply that Cnm may also facilitate recognition of endothelial receptors. Direct binding to endothelial cell lines in vitro has been demonstrated previously for Streptococcus bacteria, [41][42][43][44] but this has yet to be considered as a potential mechanism in IE. Such a possibility is worthy of investigation in future studies.…”

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confidence: 99%

Getting to the heart of the matter: Role ofStreptococcus mutansadhesin Cnm in systemic disease

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2016

Virulence

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Interactions between endocarditis-derived Streptococcus gallolyticus subsp. gallolyticus isolates and human endothelial cells

Cited by 44 publications

References 53 publications

The Collagen-Binding Protein Cnm Is Required for Streptococcus mutans Adherence to and Intracellular Invasion of Human Coronary Artery Endothelial Cells

The Collagen-Binding Protein Cnm Is Required for Streptococcus mutans Adherence to and Intracellular Invasion of Human Coronary Artery Endothelial Cells

Streptococcus gallolyticus subsp. gallolyticus from Human and Animal Origins: Genetic Diversity, Antimicrobial Susceptibility, and Characterization of a Vancomycin-Resistant Calf Isolate Carrying a vanA -Tn 1546 -Like Element

Getting to the heart of the matter: Role ofStreptococcus mutansadhesin Cnm in systemic disease

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