Interactions between ethylenediaminetetraacetic acid (EDTA) and iron absorption pathways, in the Caco-2 model

Kibangou, I.B.; Bureau, F.; Allouche, Stéphane; Arhan, Pierre; Bouglé, Dominique

doi:10.1016/j.fct.2008.08.014

Cited by 4 publications

(1 citation statement)

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“…The observations above revealed that there could be a considerable passive component in the transport of CA. EDTA is a metal ion chelator and can damage tight junction of the epithelia and increase the para-cellular permeability of hydrophilic molecules [ 40 ]. It was employed to determine whether CA has a paracellular route in the Caco-2 cell model.…”

Section: Resultsmentioning

confidence: 99%

Absorption and Transport Characteristics and Mechanisms of Carnosic Acid

Chen

Huang

et al. 2021

Biology

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Carnosic acid (CA) is a phenolic diterpenoid mainly found in rosemary and sage. CA has been reported to possess health-beneficial effects in various experimental settings. Herein, a mouse experiment and Caco-2 single-cell model were used to understand the absorption and transport characteristics of CA. First, we determined the tissue distribution of CA in mice, following an oral gavage at a physiologically relevant dose. We found that CA was bioavailable systemically and present locally in the digestive tract, especially in the cecum and colon. Next, we thought to characterize the absorption and transport of CA in the Caco-2 cell monolayer model of the intestinal epithelial barrier. In the Caco-2 cell model, CA exhibited a moderate permeability and was subjected to a mild efflux. Moreover, the apparent permeability coefficient (Papp) of CA transported across Caco-2 cell monolayers was significantly changed when the inhibitors of specific active transporter and passive diffusion were added to cells, suggesting that the absorption and transport of CA involved both passive and active transportation. The present study is an important first step towards understanding the absorption, transport, and metabolic mechanisms of CA. This could provide the scientific basis for developing CA-containing functional foods or dietary supplements with improved bioavailability.

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Section: Resultsmentioning

confidence: 99%