Interactions between human eosinophils and schistosomula of schistosoma mansoni. II. The mechanism of irreversible eosinophil adherence

Abstract

We have reported elsewhere (1) 1 that normal human eosinophils adhere to schistosomula of Schistosoma mansoni, in the presence of heat-inactivated sera from patients with schistosomiasis, to a much greater extent than do neutrophils. This finding was surprising, because neutrophils are generally considered to bear more Fc receptors (FcR) for bound IgG than do eosinophils (2-5). On further study, it became apparent that the initiation of adherence by both cell types was a temperature-independent reaction involving the cells' FcR, and that the eosinophil, but not the neutrophil, then underwent a further, temperature-dependent step which rendered its binding progressive and irreversible, 1We considered that this preferential binding of eosinophils to antibody-coated schistosomula might, in part, account for the selective damaging effect of the eosinophil, in comparison with the neutrophil (1), and that it was therefore important to study the mechanism of the irreversible step in more detail. Earlier work by ourselves (6-8) and others (9, 10) had shown that damage induced by eosinophils was associated with cell attachment, followed by degranulation and the release of granule contents onto the surface of the organism. This damage was attributable, at least in part, to the release of one major component of the eosinophil granule, the major basic protein (MBP) 2 (1 1, 12), onto the surface of the organism (13). In contrast, adherent neutrophils showed no signs of degranulation detectable at the electron microscopical level: instead, these cells formed fusions with the outer bilayer of the schistosomulum 2 Abbreviations used in this paper: Con A, eoncanavalin A; E/LAC, Earle's balanced salt solution with 0.5% lactalbumin hydrolysate; FCS, fetal calf Serum; HBSS, Hanks' balanced salt solution; MBP, eosinophil major basic protein; MEM, Eagle's minimal essential medium; NEM, n-ethylmaleimide; PBS, phosphatebuffered saline, pH 7.4. 1456J. Exp. MED.

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