Interactions Between LHRH, Sex Steroids and “Inhibin” in the Control of LH and FSH Secretion

Labrie, Fernand; Lagacé, Lisette; Ferland, Louise; Kelly, Paul A.; Drouin, J.; Massicotte, Jocelyne; Bonne, C.; Raynaud, Jean‐Pierre; Dorrington, Jennifer H.

doi:10.1111/j.1365-2605.1978.tb00008.x

Cited by 35 publications

(18 citation statements)

References 52 publications

(60 reference statements)

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“…The present study firstly demonstrated that PFF inhibited FSH secretion mainly at the pituitary level as reported by many investigators (Franchimont et al 1975;Hopkinson et al 1977;Labrie et al 1978;Lorenzen et al 1978;Depaolo et al 1979;Lumpkin et al 1981). At the same time, the long-lasting effect on basal FSH and its time course being maximal at several hours after PFF administration indicated that PFF suppressed not only FSH release but also its Huang and Miller (1984) reported that PFF rather intensified an LH-RH mediated LH release.…”

Section: Discussionsupporting

confidence: 83%

Effect of porcine follicular fluid on LH and FSH secretion in rats.

Demura

Suzuki

Nakamura

et al. 1988

Tohoku J. Exp. Med.

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Effect of Porcine Follicular Fluid on LH and FSH Secretion in Rats . Tohoku J. exp. Med., 1988, 156 (2), 157-163 -To investigate a mode and a site of action of inhibin on FSH and LH secretion , in vivo and in vitro effects of porcine follicular fluid (PFF) on FSH and LH secretion were studied in male rats . PFF administerd i.v. suppressed basal FSH levels for 1-8 hr , but did not alter LH levels. O n the contrary, PFF given either 1 or 6 hr in advance , suppressed LH-RH stimulated LH responses as well as LH-RH induced FSH responses . In cultured pituitary cells PFF exerted a partial suppressive effect on LH release. PFF suppressed LH release only under the maximal stimulation with 10-70 M LH-RH and 0.1 mM 3-isobutyl-1-metylxanthine (IBMX) to a lesser degree compared with uniform and greater suppression on FSH release regardless of the experimental conditions. Above results showed a selective suppressive effect of PFF on FSH release and synthesis with a less inhibitory effect on LH release mainly at the pituitary level.inhibin ; porcine follicular fluid ; FSH ; LH ; LH-RH It has been widely demonstrated that follicular or testicular preparations with definite inhibin activities suppressed FSH secretion in rats (Marder et al . 1977;Schwartz and Charming 1977) as well as in other species (Channing et al. 1981 ;Rettori et al. 1982). However, effects of inhibin on LH release are still controversial.To investigate the effect of inhibin on LH and FSH secretion, in vivo and in vitro effects of porcine follicular fluid (PFF) on LH and FSH secretion were studied in male rats under various conditions.

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Section: Discussionsupporting

confidence: 83%

Effect of porcine follicular fluid on LH and FSH secretion in rats.

Demura

Suzuki

Nakamura

et al. 1988

Tohoku J. Exp. Med.

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“…A stimulatory effect on the basal secretion of FSH has been observed for progesterone (Erickson & Hsueh, 1978 ;Labrie et al, 1978 ;Drouin & Labrie, 1981), androgens and oestradiol (Steinberger & Chowdhury, 1974;Labrie et al, 1978;de Jong et al, 1979), but an inhibitory action was described for androgens and no effect for oestradiol (Drouin & Labrie, 1981 ). These variable effects of steroids on the basal secretion of FSH could explain the fact that the inhibition caused by charcoal : dextran adsorption of ovarian-cell culture medium when most of the steroids are removed is greater, smaller or similar to the suppression exerted by the untreated medium.…”

Section: Discussionmentioning

confidence: 92%

“…Androgens have been known to enhance the inhibitory activity of charcoal : dextran-adsorbed bull seminal plasma in vivo (Peek & Watkins, 1980) and porcine follicular fluid in vitro (Lagace et al, 1979). Baker et al (1978), Labrie et al (1978) and found that androgens decrease the LHRH-induced release of FSH and LH. This effect of androgens could be related to a decrease in the LHRH-binding sites (Giguere, Lefevre & Labrie, 1981).…”

Section: Discussionmentioning

confidence: 98%

Biological determination of inhibin in rat ovarian-cell culture medium

Croze¹,

Franchimont²

1984

Reproduction

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Rat ovarian-cell culture medium contains steroids and inhibin material that are able to influence basal and LHRH-stimulated secretion of FSH and LH from dispersed pituitary cells. Studies on procedures to remove steroids, such as ethyl acetate extraction and charcoal: dextran adsorption, showed that adsorption of the culture medium with 0.5 to 1.0% charcoal and 0.05 to 0.1% dextran, respectively, was the optimal method to eliminate most of the steroids in the medium without a detrimental effect on inhibin activity. The inclusion of serum or bovine albumin in the culture medium after the culture protected the inhibin from adsorption by the charcoal: dextran treatment. The inhibin present in the rat ovarian-cell culture medium treated with 1.0% charcoal: 0.1% dextran behaved like the inhibin reference standard (from rete testis fluid of the sheep: RTFS) in the basal secretion of FSH and the LHRH-stimulated secretion of FSH and LH in the in-vitro bioassay.

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“…In the human, a pretreatment with an antiestrogen like clomiphene abolishes the inhibitory effect of tes tosterone on FSH and LH secretion [Winter et al, 1979], The in vivo effects of androgens on gonad otrophin secretion are the result of a hypo thalamic and pituitary' action. Smith and Davidson [ 1967] and Labrie et al [1978] have shown that testosterone and dihydrotestoste rone diminish the hypothalamic gonadotro phin releasing hormone secretion. At the pi tuitary level, androgens inhibit the secretion of LH and increase the synthesis of FSH [La brie et al, 1978].…”

Section: Direct Feedbackmentioning

confidence: 99%

Regulation of Gonadal Androgen Secretion

Franchimont¹

1983

Horm Res

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The various mechanisms regulating testicular and ovarian androgen secretion are reviewed. Testicular androgen secretion is controlled by luteinizing hormone (LH) and follicle stimulating hormone (FSH), which influence the Leydig cell response to the LH. The contribution of prolactin, growth hormone and thyroid hormones to the Leydig cell function is discussed. The ovarian androgen secretion is regulated in a very similar fashion as the Leydig cell of the testis. Prolactin, however, has an inhibitory effect on androgen secretion in the ovary. The intratesticular action of androgens is linked to spermatogenesis. Sertoli cells, by producing the androgen-binding protein, contribute to the intratubular androgen concentration. Inhibin production of the Sertoli cell is stimulated by androgens. In the ovary, androgens produced by the theca interna are used as precursors for the aromatization of estradiol, which stimulates together with FSH the mitosis of granulosa cells. The feedback control of androgen secretion is complicated, as the direct feedback mechanisms are joined by indirect feedback regulations like the peptide inhibin, which can be stimulated by androgens. Intragonadal mechanisms regulating androgen production are the cybernins for testicles and ovaries. In the testicle, estrogens from the Sertoli cells regulate the Leydig cell testosterone biosynthesis. In the ovary, nonaromatizable androgens are potent inhibitors of the aromatization activity in the granulosa cell. A peptide with a FSH receptor binding inhibiting activity is found in male and female gonads. Finally, LH-RH-like peptides have been found in the testicle, which are capable of inhibiting steroidogenesis. These gonadocrinins are similarly produced in granulosa cells of the ovary.

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Interactions Between LHRH, Sex Steroids and “Inhibin” in the Control of LH and FSH Secretion

Cited by 35 publications

References 52 publications

Effect of porcine follicular fluid on LH and FSH secretion in rats.

Effect of porcine follicular fluid on LH and FSH secretion in rats.

Biological determination of inhibin in rat ovarian-cell culture medium

Regulation of Gonadal Androgen Secretion

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