Interactions between lipid source and vitamin A on broiler performance, blood parameters, fat and protein deposition rate, and bone development

Savaris, Vaneila Daniele Lenhardt; Souza, Cléverson de; Wachholz, Lucas; Broch, Jomara; Polese, Clauber; Carvalho, Paulo Levi de Oliveira; Pozza, Paulo César; Eyng, Cínthia; Nunes, Ricardo Vianna

doi:10.1016/j.psj.2020.09.001

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…In newborn Angers calves, injection of vitamin A significantly increased percentages of intramuscular fat (Harris et al, 2018). In broiler chickens, fat deposition rate was increased during 1-21 days after hatching but decreased during 22-42 days by dietary supplementation of vitamin A (Savaris et al, 2020). In the current study, in ovo injection of 300 nM atRA at E5-E7 resulted in an increase of weights and percentages of adipose tissues (Figure 3).…”

Section: Discussionsupporting

confidence: 54%

“…In poultry, adipogenic differentiation in vitro can be induced by supplementation of atRA (Kim et al, 2020a,b;Lee et al, 2021). Dietary supplementation of vitamin A during early post-hatch period increased fat accretion in broiler chickens (Savaris et al, 2020). The present study aims to investigate the cellular effects of atRA on adipose growth in quail embryos as an animal model.…”

Section: Introductionmentioning

confidence: 97%

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Hypertrophy of Adipose Tissues in Quail Embryos by in ovo Injection of All-Trans Retinoic Acid

et al. 2021

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Excessive adipose accretion causes health issues in humans and decreases feed efficiency in poultry. Although vitamin A has been known to be involved in adipogenesis, effects of all-trans retinoic acid (atRA), as a metabolite of vitamin A, on embryonic adipose development have not been studied yet. Avian embryos are developing in confined egg environments, which can be directly modified to study effects of nutrients on embryonic adipogenesis. With the use of quail embryos, different concentrations of atRA (0 M to 10 μM) were injected in ovo at embryonic day (E) 9, and adipose tissues were sampled at E14. Percentages of fat pad weights in embryo weights were significantly increased in the group injected with 300 nM of atRA. Also, among three injection time points, E5, E7, or E9, E7 showed the most significant increase in weight and percentage of inguinal fat at E14. Injection of atRA at E7 increased fat cell size in E14 embryos with up-regulation of pro-adipogenic marker genes (Pparγ and Fabp4) and down-regulation of a preadipocyte marker gene (Dlk1) in adipose tissues. These data demonstrate that atRA promotes hypertrophic fat accretion in quail embryos, implying important roles of atRA in embryonic development of adipose tissues.

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Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 97%

Hypertrophy of Adipose Tissues in Quail Embryos by in ovo Injection of All-Trans Retinoic Acid

et al. 2021

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“…and C. perfringens co-infection, the serum cholesterol and uric acid contents were reduced in challenged birds. In the present study, the decrease in phosphorus and uric acid levels and increase in aspartate aminotransferase and creatine kinase activities induced with VitA supplementation indicated that excess VitA in the diet caused hepatic, cardiac and renal damage [ 29 ]. On the contrary, VitA supplementation increased serum low-density lipoprotein content regardless of NE challenge and reduced alkaline phosphatase activity in NE-challenged birds, which exhibited beneficial effects on lipid transportation and hepatobiliary function.…”

Section: Discussionmentioning

confidence: 97%

Effects of Vitamin A on Immune Responses and Vitamin A Metabolism in Broiler Chickens Challenged with Necrotic Enteritis

Guo

Zhang

et al. 2023

Life

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Necrotic enteritis (NE) is an important enteric inflammatory disease of poultry, and the effects of vitamin A (VitA) on NE birds are largely unknown. The present study was conducted to investigate the effects of VitA on the immune responses and VitA metabolism of NE broilers as well as the underlying mechanisms. Using a 2 × 2 factorial arrangement, 336 1-day-old Ross 308 broiler chicks were randomly assigned to 4 groups with 7 replicates. Broilers in the control (Ctrl) group were fed a basal diet without extra VitA supplementation. Broilers in the VitA group were fed a basal diet supplemented with 12,000 IU/kg of VitA. Birds in NE and VitA + NE groups were fed corresponding diets and, in addition, co-infected with Eimeria spp. and Clostridium perfringens on days 14 to 20. Samples of the blood, jejunum, spleen and liver were obtained on day 28 for analysis, and meanwhile, lesion scores were also recorded. The results showed that NE challenge increased lesion score in the jejunum and decreased serum glucose, total glyceride, calcium, phosphorus and uric acid levels (p < 0.05). VitA supplementation reduced the levels of serum phosphorus, uric acid and alkaline phosphatase in NE-challenged birds and increased serum low-density lipoprotein content and the activity of aspartate aminotransferase and creatine kinase (p < 0.05). Compared with the Ctrl group, the VitA and NE groups had higher mRNA expression of interferon-γ in the jejunum (p < 0.05). NE challenge up-regulated mRNA expression of interleukin (IL)-13, transforming growth factor-β4, aldehyde dehydrogenase (RALDH)-2 and RALDH-3 in the jejunum, while VitA supplementation increased jejunal IL-13 mRNA expression and hepatic VitA content, but down-regulated splenic IL-13 mRNA expression (p < 0.05). The VitA + NE group had higher serum prostaglandin E2 levels and the Ctrl group had higher splenic RALDH-3 mRNA expression than that of the other three groups (p < 0.05). NE challenge up-regulated jejunal retinoic acid receptor (RAR)-β and retinoid X receptor (RXR)-α as well as splenic RAR-α and RAR-β mRNA expression (p < 0.05). VitA supplementation up-regulated jejunal RAR-β expression but down-regulated mRNA expression of RXR-α, RXR-γ, signal transducers and activators of transcription (STAT) 5 and STAT6 in the spleen (p < 0.05). Moreover, compared with the Ctrl group, the VitA and NE groups had down-regulated mRNA expression of jejunal and splenic Janus kinase (JAK) 1 (p < 0.05). In conclusion, NE challenge induced jejunal injury and expression of Th2 and Treg cell-related cytokines and enhanced RALDH and RAR/RXR mRNA expression, mainly in the jejunum of broilers. VitA supplementation did not alleviate jejunal injury or Th2 cell-related cytokine expression; however, it improved hepatic VitA deposition and inhibited the expression of RALDH-3, RXR and the JAK/STAT signaling pathway in the spleen of broilers. In short, the present study suggested the modulatory effects of vitamin A on the immune responses and vitamin A metabolism in broiler chickens challenged with necrotic enteritis.

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“…The possible mechanisms might be summarized as follows: First, vitamin A could indirectly improve bone health by restraining the excessive secretion of parathyroid hormone, a higher level of which was suggested to result in poor bone health [ 23 , 24 ]. Second, vitamin A can promote bone growth by means of affecting the growth hormone and stimulating the production of insulin-like growth factor 1, which are conducive to bone health [ 25 ]. Third, oxidative stress and reactive oxygen species can promote the development of osteoporosis.…”

Section: Discussionmentioning

confidence: 99%

Associations of serum vitamins levels with bone mineral density in the different race-ethnicities US adults

Liu

2021

BMC Musculoskelet Disord

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Background The conclusions on the associations of specific vitamin levels with bone mineral density (BMD) were controversial. Therefore, the aims of this study were to examine the associations of serum vitamins levels with BMD and the modified effect of race/ ethnicity on these associations in the US adults. Methods This study was from the third National Health and Nutrition Examination Survey. All participants aged ≥18 years with complete data were eligible. Serum vitamins A, B9, B12, C, and E levels were assayed using the Quantaphase II Radioassay Kit (Bio-Rad). Dual-energy X-ray absorptiometry was employed to measure BMD, including femur neck and the total hip. Results There were 6023 participants included in the final analysis. Serum folate, vitamins A and C levels were positively associated with BMD. No significant associations of serum vitamins B12 and E levels with BMD were observed. There were positive associations of serum folate level (β = 0.00027 and 0.00032; and 95% CI: 0.00002–0.00057 and 0.00002–0.00063, respectively), vitamin A level (β = 0.01132 and 0.01115; and 95% CI: 0.00478–0.01787 and 0.00430–0.01799, respectively), and vitamin C level (β = 0.00027 and 0.00029; and 95% CI: 0.00012–0.00042 and 0.00013–0.00045, respectively) with BMD at femur neck and the total hip only in the Not Hispanic participants. Conclusion Elevated serum folate, vitamins A and C levels were associated with a higher BMD. Furthermore, sex and race/ ethnicity modified the associations of serum vitamins levels with BMD.

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Interactions between lipid source and vitamin A on broiler performance, blood parameters, fat and protein deposition rate, and bone development

Cited by 6 publications

References 20 publications

Hypertrophy of Adipose Tissues in Quail Embryos by in ovo Injection of All-Trans Retinoic Acid

Hypertrophy of Adipose Tissues in Quail Embryos by in ovo Injection of All-Trans Retinoic Acid

Effects of Vitamin A on Immune Responses and Vitamin A Metabolism in Broiler Chickens Challenged with Necrotic Enteritis

Associations of serum vitamins levels with bone mineral density in the different race-ethnicities US adults

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