Interactions between marine bacteria and dissolved-phase and beached hydrocarbons after the Exxon Valdez oil spill

Button, D. K.; Robertson, Betsy R.; McIntosh, Douglas J.; Jüttner, Friedrich

doi:10.1128/aem.58.1.243-251.1992

Cited by 52 publications

(12 citation statements)

References 30 publications

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“…The very large value for toluene affinity is consistent with the idea that permeases are not required for hydrocarbon transport but, rather, that such lipid-soluble substrates diffuse directly to a dioxygenase for initial reaction (14). However, some workers suggest that such substrates may be transported ac- a Conversion factors used: 400 mg/liter (wet wt) OD Ϫ1 (30), 3 mg of cells (wet wt) (mg dry wt) Ϫ1 , 1.9 mg of cells (dry wt) mg of protein Ϫ1 .…”

Section: Data Interpretationssupporting

confidence: 77%

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Nutrient Uptake by Microorganisms according to Kinetic Parameters from Theory as Related to Cytoarchitecture

Button

1998

Microbiol Mol Biol Rev

139

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SUMMARY The abilities of organisms to sequester substrate are described by the two kinetic constants specific affinity, a°, and maximal velocity Vmax. Specific affinity is derived from the frequency of substrate-molecule collisions with permease sites on the cell surface at subsaturating concentrations of substrates. Vmax is derived from the number of permeases and the effective residence time, τ, of the transported molecule on the permease. The results may be analyzed with affinity plots (v/S versus v, where v is the rate of substrate uptake), which extrapolate to the specific affinity and are usually concave up. A third derived parameter, the affinity constant KA, is similar to KM but is compared to the specific affinity rather than Vmax and is defined as the concentration of substrate necessary to reduce the specific affinity by half. It can be determined in the absence of a maximal velocity measurement and is equal to the Michaelis constant for a system with hyperbolic kinetics. Both are taken as a measure of τ, with departure of KM from KA being affected by permease/enzyme ratios. Compilation of kinetic data indicates a 108-fold range in specific affinities and a smaller (103-fold) range in Vmax values. Data suggest that both specific affinities and maximal velocities can be underestimated by protocols which interrupt nutrient flow prior to kinetic analysis. A previously reported inverse relationship between specific affinity and saturation constants was confirmed. Comparisons of affinities with ambient concentrations of substrates indicated that only the largest a°S values are compatible with growth in natural systems.

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Section: Data Interpretationssupporting

confidence: 77%

“…Only the uppermost value of Fig. 3 is sufficient for growth on a single substrate in natural water systems at ambient measured concentrations of substrate which are near 1 g/liter (14,16).…”

Section: Data Interpretationsmentioning

confidence: 96%

Nutrient Uptake by Microorganisms according to Kinetic Parameters from Theory as Related to Cytoarchitecture

Button

1998

Microbiol Mol Biol Rev

139

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“…Robertson and Button (43) observed a continued increase in the blue fluorescence of heterotrophic bacteria at concentrations above 1.0 ,g of DAPI ml-' and used 2.5 ,ug ml-' for best results. However, 0.5 ,ug of DAPI ml-1 has been used in subsequent work by these authors (8). While it is not strictly valid to extrapolate beyond the dye concentrations used in this study, the flatness of the abundance curve beyond 0.5 ,ug ml-' (Fig.…”

Section: Discussionmentioning

confidence: 89%

Flow Cytometric Analysis of Marine Bacteria with Hoechst 33342

Monger

Landry

1993

Appl Environ Microbiol

175

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We investigated the accuracy and precision of flow cytometric (FCM) estimates of bacterial abundances using 4',6-diamidino-2-phenylindole (DAPI) and Hoechst 33342 (HO342, a bisbenzamide derivative) on paraformaldehyde-fixed seawater samples collected from two stations near Oahu, Hawaii. The accuracy of FCM estimates was assessed against direct counts by using epifluorescence microscopy. DAPI and H0342 differ in two aspects of their chemistry that make H0342 better suited for staining marine heterotrophic bacteria for FCM analysis. These differences are most important in studies of open-ocean ecosystems that require dual-beam FCM analysis to clearly separate heterotrophic bacterial populations from populations of photosynthetic Prochlorococcus spp. Bacterial populations were easier to distinguish from background fluorescence when stained with H0342 than when stained with DAPI, because H0342 has a higher relative fluorescence quantum yield. A substantially higher coefficient of variation of blue fluorescence, which was probably due to fluorescent complexes formed by DAPI with double-stranded RNA, was observed for DAPI-stained populations. FCM estimates averaged 2.0 and 12% higher than corresponding epifluorescence microscopy direct counts for H0342 and DAPI-stained samples, respectively. A paired-sample t test between FCM estimates and direct counts found no significant difference for H0342-stained samples but a significant difference for DAPI-stained samples. Coefficients of variation of replicate FCM abundance estimates ranged from 0.63 to 2.9%o (average, 1.5%) for natural bacterial concentrations of 6 x 105 to 15 x 105 cells ml-'.

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“…Button et al attributed the low K, of a marine Pseudomonas sp. with toluene to its partitioning into the membrane (7). Benson et al (2) showed that components of the alkane hydroxylase of Pseudomonas putida, which converts hydrophobic alkanes to readily water-soluble alkanols, are located in the cytoplasm membrane.…”

Section: Discussionmentioning

confidence: 99%

Influence of substrate diffusion on degradation of dibenzofuran and 3-chlorodibenzofuran by attached and suspended bacteria

Harms

Zehnder

1994

Appl Environ Microbiol

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Dibenzofuran uptake-associated kinetic parameters of suspended and attached Sphingomonas sp. strain HH19k cells were compared. The suspended cells were studied in a batch system, whereas glass beads in percolated columns were used as the solid support for attached cells. The maximum specific activities of cells in the two systems were the same. The apparent half-maximum uptake rate-associated concentrations (K,') of attached cells, however, were considerably greater than those of suspended cells and depended on cell density and on percolation velocity. A mathematical model was developed to explain the observed differences in terms of substrate transport to the cells. This model was based on the assumptions that the intrinsic half-maximum uptake rate-associated concentration (K,) was unchanged and that deviations of K,' from K, resulted from the stereometry and the hydrodynamics around the cells. Our calculations showed that (i) diffusion to suspended cells and to single attached cells is efficient and therefore only slightly affects K,'; (ii) diffusion to cells located on crowded surfaces is considerably lower than that to single attached cells and greatly increases K,', which depends on the cell density; (iii) the convective-diffusive transport to attached cells that occurs in a percolated column is influenced by the liquid flow and results in dependency ofK,' on the flow rate; and (iv) higher specific affinity of cells correlates with higher susceptibility to diffusion limitation. Properties of the experimental system which limited quantitative proof of exclusively transport-controlled variations of K,' are discussed.

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Interactions between marine bacteria and dissolved-phase and beached hydrocarbons after the Exxon Valdez oil spill

Cited by 52 publications

References 30 publications

Nutrient Uptake by Microorganisms according to Kinetic Parameters from Theory as Related to Cytoarchitecture

Nutrient Uptake by Microorganisms according to Kinetic Parameters from Theory as Related to Cytoarchitecture

Flow Cytometric Analysis of Marine Bacteria with Hoechst 33342

Influence of substrate diffusion on degradation of dibenzofuran and 3-chlorodibenzofuran by attached and suspended bacteria

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