Interactions between Meloidogyne incognita and endophytic bacteria in cotton and cucumber

Hallmann, Johannes; Quadt-Hallmann, A.; Rodrı́guez-Kábana, R.; Kloepper, Joseph W.

doi:10.1016/s0038-0717(97)00183-1

Cited by 106 publications

(51 citation statements)

References 16 publications

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“…cubense. The positive regulation of phytopathogen on associated endophyte population is also pointed at by several researchers (Hallmann et al, 1998;Reiter et al, 2002). In that case, pathogens in plants would induce a cascade of reactions leading to the synthesis of stress metabolites, including H 2 O 2 , phytoalexins, and stress signals such as abscisic acid, jasmonic acid, and salicylic acid (Lichtenthaler, 1998); all of these would make changes in endophyte structure.…”

Section: Discussionmentioning

confidence: 89%

Response of endophytic bacterial communities in banana tissue culture plantlets to Fusarium wilt pathogen infection

Liu

Wang

Zhou

2008

J. Gen. Appl. Microbiol.

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Endophytic bacteria reside within plant hosts without having pathogenic effects, and various endophytes have been found to functionally benefit plant disease suppressive ability. In this study, the influence of banana plant stress on the endophytic bacterial communities, which was achieved by infection with the wilt pathogen Fusarium oxysporum f. sp. cubense, was examined by cultivation-independent denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA directly amplified from plant tissue DNA. Community analysis clearly demonstrated increased bacterial diversity in pathogen-infected plantlets compared to that in control plantlets. By sequencing, bands most similar to species of Bacillus and Pseudomonas showed high density in the pathogen-treated pattern. In vitro screening of the isolates for antagonistic activity against Fusarium wilt pathogen acquired three strains of endophytic bacteria which were found to match those species that obviously increased in the pathogen infection process; moreover, the most inhibitive strain could also interiorly colonize plantlets and perform antagonism. The evidence obtained from this work showed that antagonistic endophytic bacteria could be induced by the appearance of a host fungal pathogen and further be an ideal biological control agent to use in banana Fusarium wilt disease protection.

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Section: Discussionmentioning

confidence: 89%

Response of endophytic bacterial communities in banana tissue culture plantlets to Fusarium wilt pathogen infection

Liu

Wang

Zhou

2008

J. Gen. Appl. Microbiol.

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“…The Grampositive obligate parasites of root knot nematodes, Pasteuria spp., are highly host specific in endospore attachment to the cuticle. Thus far, only a few examples for nonparasitic attachment of bacteria or fungi to the cuticle of plant-parasitic nematodes have been described (25,26), and images of the J2 surface by scanning electron microscopy indicated a rather low abundance of microorganisms with the exception of highly specialized parasites (27). Also, we found evidence for a rather low number of microbes on the cuticle, evidenced by high variation between microbial DGGE fingerprints from J2, and low amounts of direct PCR products from DNA of J2 samples.…”

Section: Discussionmentioning

confidence: 99%

Specific Microbial Attachment to Root Knot Nematodes in Suppressive Soil

Adam

Westphal

Hallmann

et al. 2014

Appl Environ Microbiol

116

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bUnderstanding the interactions of plant-parasitic nematodes with antagonistic soil microbes could provide opportunities for novel crop protection strategies. Three arable soils were investigated for their suppressiveness against the root knot nematode Meloidogyne hapla. For all three soils, M. hapla developed significantly fewer galls, egg masses, and eggs on tomato plants in unsterilized than in sterilized infested soil. Egg numbers were reduced by up to 93%. This suggested suppression by soil microbial communities. The soils significantly differed in the composition of microbial communities and in the suppressiveness to M. hapla. To identify microorganisms interacting with M. hapla in soil, second-stage juveniles (J2) baited in the test soil were cultivation independently analyzed for attached microbes. PCR-denaturing gradient gel electrophoresis of fungal ITS or 16S rRNA genes of bacteria and bacterial groups from nematode and soil samples was performed, and DNA sequences from J2-associated bands were determined. The fingerprints showed many species that were abundant on J2 but not in the surrounding soil, especially in fungal profiles. Fungi associated with J2 from all three soils were related to the genera Davidiella and Rhizophydium, while the genera Eurotium, Ganoderma, and Cylindrocarpon were specific for the most suppressive soil. Among the 20 highly abundant operational taxonomic units of bacteria specific for J2 in suppressive soil, six were closely related to infectious species such as Shigella spp., whereas the most abundant were Malikia spinosa and Rothia amarae, as determined by 16S rRNA amplicon pyrosequencing. In conclusion, a diverse microflora specifically adhered to J2 of M. hapla in soil and presumably affected female fecundity.

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“…The possibility that Enterobacter asburiae is a competitor of S. enterica serovar Newport and E. coli O157 on A. thaliana is indicated by the observation that its appearance on the plant was correlated with the highest competition against S. enterica serovar Newport and E. coli O157:H7. Enterobacter asburiae has been reported to be an endophyte and a frequent inhabitant of the rhizosphere of cotton, bean, and cucumber (23,24,39) and to move systemically in the plant (46). Evidence that Enterobacter asburiae inhabits the phyllosphere has not been reported.…”

Section: Discussionmentioning

confidence: 99%

Colonization of Arabidopsis thaliana with Salmonella enterica and Enterohemorrhagic Escherichia coli O157:H7 and Competition by Enterobacter asburiae

Cooley

Miller

Mandrell

2003

Appl Environ Microbiol

265

217

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Enteric pathogens, such as Salmonella enterica and Escherichia coli O157:H7, have been shown to contaminate fresh produce. Under appropriate conditions, these bacteria will grow on and invade the plant tissue. We have developed Arabidopsis thaliana (thale cress) as a model system with the intention of studying plant responses to human pathogens. Under sterile conditions and at 100% humidity, S. enterica serovar Newport and E. coli O157:H7 grew to 10 9 CFU g ؊1 on A. thaliana roots and to 2 ؋ 10 7 CFU g ؊1 on shoots. Furthermore, root inoculation led to contamination of the entire plant, indicating that the pathogens are capable of moving on or within the plant in the absence of competition. Inoculation with green fluorescent protein-labeled S. enterica and E. coli O157:H7 showed invasion of the roots at lateral root junctions. Movement was eliminated and invasion decreased when nonmotile mutants of S. enterica were used. Survival of S. enterica serovar Newport and E. coli O157:H7 on soil-grown plants declined as the plants matured, but both pathogens were detectable for at least 21 days. Survival of the pathogen was reduced in unautoclaved soil and amended soil, suggesting competition from indigenous epiphytes from the soil. Enterobacter asburiae was isolated from soil-grown A. thaliana and shown to be effective at suppressing epiphytic growth of both pathogens under gnotobiotic conditions. Seed and chaff harvested from contaminated plants were occasionally contaminated. The rate of recovery of S. enterica and E. coli O157:H7 from seed varied from undetectable to 19% of the seed pools tested, depending on the method of inoculation. Seed contamination by these pathogens was undetectable in the presence of the competitor, Enterobacter asburiae. Sampling of 74 pools of chaff indicated a strong correlation between contamination of the chaff and seed (P ‫؍‬ 0.025). This suggested that contamination of the seed occurred directly from contaminated chaff or by invasion of the flower or silique. However, contaminated seeds were not sanitized by extensive washing and chlorine treatment, indicating that some of the bacteria reside in a protected niche on the seed surface or under the seed coat.

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Interactions between Meloidogyne incognita and endophytic bacteria in cotton and cucumber

Cited by 106 publications

References 16 publications

Response of endophytic bacterial communities in banana tissue culture plantlets to Fusarium wilt pathogen infection

Response of endophytic bacterial communities in banana tissue culture plantlets to Fusarium wilt pathogen infection

Specific Microbial Attachment to Root Knot Nematodes in Suppressive Soil

Colonization of Arabidopsis thaliana with Salmonella enterica and Enterohemorrhagic Escherichia coli O157:H7 and Competition by Enterobacter asburiae

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