Interactions between mycoplasma lipid-associated membrane proteins and the host cells

You, Xiaoxing; Zeng, Yanhua; Wu, Yimou

doi:10.1631/jzus.2006.b0342

Cited by 32 publications

(27 citation statements)

References 37 publications

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“…Mycoplasmas are bacteria with the smallest genome (580-2200 kb), with a relatively high A þ T content (67-76%) [1]. With this basic genome, the bacteria have to obtain most nutrients from the host cells.…”

Section: Introductionmentioning

confidence: 99%

Mycoplasma genitaliumlipoproteins inhibit tumour necrosis factor α-induced apoptosis in HeLa cells

Zuo

Sun

et al. 2018

Biotechnology & Biotechnological Equipment

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This aim of this study was to investigate the effects of Mycoplasma genitalium lipoproteins on the tumour necrosis factor a (TNF-a)-induced apoptosis in HeLa cells. We separated the M. genitalium lipid-associated membrane proteins (LAMPs) from M. genitalium and used them to treat HeLa cells. Apoptosis was induced by TNF-a treatment. Cell cycle and apoptosis were detected by flow cytometry. Pro-inflammatory cytokine contents were determined by enzymelinked immunosorbent assay (ELISA). Mitochondrial membrane potential and caspase-3 protease activity were also measured. There was no significant difference in the viable cell percentage in HeLa cells treated with M. genitalium LAMPs at 0-20 lg/mL. However, when treated with 40 lg/mL LAMPs, cytocidal activity was observed during the first 24 h. M. genitalium LAMPs induced cell cycle arrest at the G 1 phase in HeLa cells. When apoptosis was induced by TNF-a in HeLa cells, M. genitalium LAMPs significantly reduced the percentage of apoptotic cells. In addition, M. genitalium LAMPs significantly reduced the loss of mitochondrial membrane potential and caspase-3 protease activity in TNF-a-treated HeLa cells. However, the blocking of NF-jB significantly increased the mitochondrial membrane potential and caspase-3 protease activity in TNF-a-induced HeLa cells treated with M. genitalium LAMPs. The obtained results suggest that M. genitalium LAMPs could inhibit TNF-a-induced apoptosis in HeLa cells, which would contribute to the understanding of the pathogenesis of M. genitalium. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Mycoplasma genitaliumlipoproteins inhibit tumour necrosis factor α-induced apoptosis in HeLa cells

Zuo

Sun

et al. 2018

Biotechnology & Biotechnological Equipment

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“…Despite of its simple structure and function, the way that ureaplasmas interact with their host is not totally elucidated, although studies demonstrated that the way of parasitism in the host cell is primarily extra-cellular (You et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Histopatology of the reproductive tract of Nellore pubertal heifers with genital ureaplasmosis

Porto

Filho

Ferraz

et al. 2017

An. Acad. Bras. Ciênc.

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In order to study and characterize the lesions in the reproductive tract of Nellore heifers naturally infected with Ureaplasma diversum and presenting granular vulvovaginitis syndrome (GVS), fragments of uterine tube, uterus, cervix, vagina and vulva of 20 animals were evaluated. The macroscopic lesions of the vulvovaginal mucosa were classified in scores of "1" mild, until "4", severe inflammation and pustular or necrotic lesions. The histopathological evaluation was performed using scores of "1" to "4", according to the inflammatory alterations. The fragments with severe microscopic lesions (3 and 4) were from the uterine tubes and uterus, which showed leukocytes infiltration and destruction and/or necrosis of epithelium. Alterations in the lower reproductive tract fragments were mild, but characteristics of acute inflammatory processes. The histopathological findings of the reproductive tract of females naturally infected with Ureaplasma diversum are consistent with injuries that compromise the environment from the local where spermatozoa acquires ability to fertilize an oocyte until those where the oocyte is fertilized. Therefore, animals with GVS should be identified early in the herd, because, besides the reduction in the fertility rates caused by tissue damages, they can contribute to disseminate the microorganism.

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“…The Mycoplasma is bound by a single limiting cytoplasmic membrane with abundant lipoproteins known as lipid-associated membrane proteins (LAMPs) (You et al, 2006). Most of the LAMPs are exposed to the extracellular milieu and some have either been described as virulence factors (Szczepanek et al, 2010) or targets for antibodies (Washburn et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis induced by lipid-associated membrane proteins from Mycoplasma hyopneumoniae in a porcine lung epithelial cell line with the involvement of caspase 3 and the MAPK pathway

Ni¹,

Bai²,

Wei³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Lipid-associated membrane proteins (LAMPs) are important in the pathogenicity of the Mycoplasma genus of bacteria. We investigated whether Mycoplasma hyopneumoniae LAMPs have pathogenic potential by inducing apoptosis in a St. Jude porcine lung epithelial cell line (SJPL). LAMPs from a pathogenic strain of M. hyopneumoniae (strain 232) were used in the research. Our investigation made use of diamidino-phenylindole (DAPI) and acridine orange/ethidium bromide (AO/EB) staining, terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) analysis, B. Ni et al. 11430©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 11429-11443 (2015) and Annexin-V-propidium iodide staining. After LAMP treatment for 24 h, typical changes were induced, chromosomes were concentrated, apoptotic bodies were observed, the 3'-OH groups of cleaved genomes were exposed, and the percentage of apoptotic cells reached 36.5 ± 11.66%. Caspase 3 and caspase 8 were activated and cytochrome c (cyt c) was released from the mitochondria into the cytoplasm; poly ADP ribose polymerase (PARP) was digested into two fragments; p38 mitogen-activated protein kinase (MAPK) was phosphorylated; and the expression of pro-apoptosis protein Bax increased while the antiapoptosis protein Bcl-2 decreased. LAMPs also stimulated SJPL cells to produce nitric oxide (NO) and superoxide. This study demonstrated that LAMPs from M. hyopneumoniae can induce apoptosis in SJPL cells through the activation of caspase 3, caspase 8, cyt c, Bax, and p38 MAPK, thereby contributing to our understanding of the pathogenesis of M. hyopneumoniae, which should improve the treatment of M. hyopneumoniae infections.

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Interactions between mycoplasma lipid-associated membrane proteins and the host cells

Cited by 32 publications

References 37 publications

Mycoplasma genitaliumlipoproteins inhibit tumour necrosis factor α-induced apoptosis in HeLa cells

Mycoplasma genitaliumlipoproteins inhibit tumour necrosis factor α-induced apoptosis in HeLa cells

Histopatology of the reproductive tract of Nellore pubertal heifers with genital ureaplasmosis

Apoptosis induced by lipid-associated membrane proteins from Mycoplasma hyopneumoniae in a porcine lung epithelial cell line with the involvement of caspase 3 and the MAPK pathway

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