Interactions between Penicillium brevicompactum/Penicillium expansum and Acinetobacter calcoaceticus isolated from drinking water in biofilm development and control

Simões, Lúcia C.; Chaves, Ana F. A.; Simões, Manuel; Lima, Nelson

doi:10.1016/j.ijfoodmicro.2022.109980

Cited by 5 publications

(1 citation statement)

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“…The design of microbial cultivation experiments for laboratory classes typically disregards the role of the relative dimension of the hydrodynamic forces, i.e., experiments are carried out typically with microorganisms cultivated under static or (unspecific) shaking conditions. However, the hydrodynamic conditions under which microorganisms are exposed influence microbial dynamics and behavior, namely motility, morphology, growth kinetics, metabolic activity, and biofilm formation ( 6 – 9 ).…”

Section: Introductionmentioning

confidence: 99%

A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks

Simões,

Oliveira,

Borges

et al. 2023

J Microbiol Biol Educ.

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Shake flask cultivation is a routine technique in microbiology and biotechnology laboratories where cell growth can be affected by the hydrodynamic conditions, which depend on the agitation velocity, shaking diameter, and shake flask size. Liquid agitation is implemented inherently to increase aeration, substrate transfer to the cells, and prevent sedimentation, disregarding the role of hydrodynamics in microbial growth and metabolism. Here, we present a simple approach to help standardize the hydrodynamic forces in orbital shakers to increase the experimental accuracy and reproducibility and give students a better knowledge of the significance of the agitation process in microbial growth.

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Section: Introductionmentioning

confidence: 99%